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32 relations: Aspergillus nuclease S1, Cell nucleus, Cloning, Cloning vector, Complementary DNA, DNA ligase, DNA polymerase, Enhancer (genetics), Escherichia coli, Eukaryote, FANTOM, Functional cloning, Genomic library, Histone, Intron, Library (biology), Messenger RNA, Plasmid, Polyadenylation, Primer (molecular biology), Restriction enzyme, Reverse genetics, Reverse transcriptase, Ribonuclease, Ribosomal RNA, RNA splicing, Spin column-based nucleic acid purification, Stem-loop, Sticky and blunt ends, Transcriptome, Transfer RNA, Trizol.
Aspergillus nuclease S1
Aspergillus nuclease S1 is an endonuclease enzyme derived from Aspergillus oryzae that splits single-stranded DNA (ssDNA) and RNA into oligo- or mononucleotides.
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Cell nucleus
In cell biology, the nucleus (pl. nuclei; from Latin nucleus or nuculeus, meaning kernel or seed) is a membrane-enclosed organelle found in eukaryotic cells.
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Cloning
Cloning is the process of producing genetically identical individuals of an organism either naturally or artificially.
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Cloning vector
A cloning vector is a small piece of DNA, taken from a virus, a plasmid, or the cell of a higher organism, that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes.
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Complementary DNA
In genetics, complementary DNA (cDNA) is DNA synthesized from a single stranded RNA (e.g., messenger RNA (mRNA) or microRNA) template in a reaction catalyzed by the enzyme reverse transcriptase.
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DNA ligase
DNA ligase is a specific type of enzyme, a ligase, that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond.
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DNA polymerase
DNA polymerases are enzymes that synthesize DNA molecules from deoxyribonucleotides, the building blocks of DNA.
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Enhancer (genetics)
In genetics, an enhancer is a short (50–1500 bp) region of DNA that can be bound by proteins (activators) to increase the likelihood that transcription of a particular gene will occur.
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Escherichia coli
Escherichia coli (also known as E. coli) is a Gram-negative, facultatively anaerobic, rod-shaped, coliform bacterium of the genus Escherichia that is commonly found in the lower intestine of warm-blooded organisms (endotherms).
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Eukaryote
Eukaryotes are organisms whose cells have a nucleus enclosed within membranes, unlike Prokaryotes (Bacteria and other Archaea).
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FANTOM
FANTOM (Functional Annotation of the Mouse/Mammalian Genome) is an international research consortium first established in 2000 as part of the RIKEN research institute in Japan.
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Functional cloning
Functional cloning is a molecular cloning technique that relies on prior knowledge of the encoded protein’s sequence or function for gene identification.
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Genomic library
A genomic library is a collection of the total genomic DNA from a single organism.
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Histone
In biology, histones are highly alkaline proteins found in eukaryotic cell nuclei that package and order the DNA into structural units called nucleosomes.
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Intron
An intron is any nucleotide sequence within a gene that is removed by RNA splicing during maturation of the final RNA product.
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Library (biology)
In molecular biology, a library is a collection of DNA fragments that is stored and propagated in a population of micro-organisms through the process of molecular cloning.
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Messenger RNA
Messenger RNA (mRNA) is a large family of RNA molecules that convey genetic information from DNA to the ribosome, where they specify the amino acid sequence of the protein products of gene expression.
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Plasmid
A plasmid is a small DNA molecule within a cell that is physically separated from a chromosomal DNA and can replicate independently.
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Polyadenylation
Polyadenylation is the addition of a poly(A) tail to a messenger RNA.
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Primer (molecular biology)
A primer is a short strand of RNA or DNA (generally about 18-22 bases) that serves as a starting point for DNA synthesis.
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Restriction enzyme
A restriction enzyme or restriction endonuclease is an enzyme that cleaves DNA into fragments at or near specific recognition sites within the molecule known as restriction sites.
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Reverse genetics
Reverse genetics is a method that is used to help understand the function of a gene by analyzing the phenotypic effects of specific engineered gene sequences.
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Reverse transcriptase
A reverse transcriptase (RT) is an enzyme used to generate complementary DNA (cDNA) from an RNA template, a process termed reverse transcription.
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Ribonuclease
Ribonuclease (commonly abbreviated RNase) is a type of nuclease that catalyzes the degradation of RNA into smaller components.
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Ribosomal RNA
Ribosomal ribonucleic acid (rRNA) is the RNA component of the ribosome, and is essential for protein synthesis in all living organisms.
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RNA splicing
In molecular biology, splicing is the editing of the nascent precursor messenger RNA (pre-mRNA) transcript into a mature messenger RNA (mRNA).
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Spin column-based nucleic acid purification
Spin column-based nucleic acid purification is a solid phase extraction method to quickly purify nucleic acids.
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Stem-loop
Stem-loop intramolecular base pairing is a pattern that can occur in single-stranded DNA or, more commonly, in RNA.
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Sticky and blunt ends
DNA ends refer to the properties of the end of DNA molecules, which may be sticky ends (cohesive ends), blunt ends or in other forms.
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Transcriptome
The transcriptome is the set of all RNA molecules in one cell or a population of cells.
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Transfer RNA
A transfer RNA (abbreviated tRNA and formerly referred to as sRNA, for soluble RNA) is an adaptor molecule composed of RNA, typically 76 to 90 nucleotides in length, that serves as the physical link between the mRNA and the amino acid sequence of proteins.
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Trizol
TRIzol is a chemical solution used in RNA/DNA/protein extraction, by the reference paper from Piotr Chomczyński and Sacchi, N. in 1987.
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CDNA clone, CDNA libraries, Cdna library.
References
[1] https://en.wikipedia.org/wiki/CDNA_library
