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41 relations: Antibody, Binding site, Cell (biology), ChIP-exo, ChIP-on-chip, ChIP-sequencing, ChiRP-Seq, Chromatin, Chromatin immunoprecipitation, Competition-ChIP, CUT&RUN sequencing, DNA, DNA sequencing, DNase-Seq, DRIP-seq, Epigenetics, FAIRE-Seq, Gene expression, Genotype, Hidden Markov model, HITS-CLIP, In vivo, Microarray, Nucleosome, Oligonucleotide, Paired-end tag, PAR-CLIP, Peak calling, Phenotype, Polymerase, Post-translational modification, Protein, Protein microarray, Pyrosequencing, RNA immunoprecipitation chip, Sono-Seq, Systematic evolution of ligands by exponential enrichment, TCP-seq, Tiling array, Transcription (biology), Transcription factor.
Antibody
An antibody (Ab), also known as an immunoglobulin (Ig), is a large, Y-shaped protein produced mainly by plasma cells that is used by the immune system to neutralize pathogens such as pathogenic bacteria and viruses.
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Binding site
In biochemistry, a binding site is a region on a protein or piece of DNA or RNA to which ligands (specific molecules and/or ions) may form a chemical bond.
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Cell (biology)
The cell (from Latin cella, meaning "small room") is the basic structural, functional, and biological unit of all known living organisms.
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ChIP-exo
ChIP-exo is a chromatin immunoprecipitation based method for mapping the locations at which a protein of interest (transcription factor) binds to the genome.
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ChIP-on-chip
ChIP-on-chip (also known as ChIP-chip) is a technology that combines chromatin immunoprecipitation ('ChIP') with DNA microarray ("chip").
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ChIP-sequencing
ChIP-sequencing, also known as ChIP-seq, is a method used to analyze protein interactions with DNA.
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ChiRP-Seq
ChiRP-Seq (Chromatin Isolation by RNA purification) is a high-throughput sequencing method to discover regions of the genome which are bound by a specific RNA (or by a ribonucleoprotein containing the RNA of interest).
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Chromatin
Chromatin is a complex of macromolecules found in cells, consisting of DNA, protein, and RNA.
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Chromatin immunoprecipitation
Chromatin immunoprecipitation (ChIP) is a type of immunoprecipitation experimental technique used to investigate the interaction between proteins and DNA in the cell.
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Competition-ChIP
Competition-ChIP is variant of the Chip-Sequencing protocol, used to measure relative binding dynamics of a transcription factor (TF) on DNA.
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CUT&RUN sequencing
CUT&RUN-sequencing, also known as Cleavage Under Targets and Release Using Nuclease, is a method used to analyze protein interactions with DNA.
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DNA
Deoxyribonucleic acid (DNA) is a thread-like chain of nucleotides carrying the genetic instructions used in the growth, development, functioning and reproduction of all known living organisms and many viruses.
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DNA sequencing
DNA sequencing is the process of determining the precise order of nucleotides within a DNA molecule.
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DNase-Seq
DNase-seq (DNase I hypersensitive sites sequencing) is a method in molecular biology used to identify the location of regulatory regions, based on the genome-wide sequencing of regions sensitive to cleavage by DNase I. FAIRE-Seq is a successor of DNase-seq for the genome-wide identification of accessible DNA regions in the genome.
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DRIP-seq
DRIP-seq (DRIP-sequencing) is a technology for genome-wide profiling of a type of DNA-RNA hybrid called an "R-loop".
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Epigenetics
Epigenetics is the study of heritable changes in gene function that do not involve changes in the DNA sequence.
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FAIRE-Seq
FAIRE-Seq (Formaldehyde-Assisted Isolation of Regulatory Elements) is a method in molecular biology used for determining the sequences of DNA regions in the genome associated with regulatory activity.
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Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product.
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Genotype
The genotype is the part of the genetic makeup of a cell, and therefore of an organism or individual, which determines one of its characteristics (phenotype).
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Hidden Markov model
Hidden Markov Model (HMM) is a statistical Markov model in which the system being modeled is assumed to be a Markov process with unobserved (i.e. hidden) states.
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HITS-CLIP
High-throughput sequencing of RNA isolated by crosslinking immunoprecipitation (HITS-CLIP, also known as CLIP-Seq) is a genome-wide means of mapping protein–RNA binding sites or RNA modification sites in vivo.
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In vivo
Studies that are in vivo (Latin for "within the living"; often not italicized in English) are those in which the effects of various biological entities are tested on whole, living organisms or cells, usually animals, including humans, and plants, as opposed to a tissue extract or dead organism.
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Microarray
A microarray is a multiplex lab-on-a-chip.
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Nucleosome
A nucleosome is a basic unit of DNA packaging in eukaryotes, consisting of a segment of DNA wound in sequence around eight histone protein cores.
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Oligonucleotide
Oligonucleotides are short DNA or RNA molecules, oligomers, that have a wide range of applications in genetic testing, research, and forensics.
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Paired-end tag
Paired-end tags (PET) (sometimes "Paired-End diTags", or simply "ditags") are the short sequences at the 5’ and 3’ ends of a DNA fragment which are unique enough that they (theoretically) exist together only once in a genome, therefore making the sequence of the DNA in between them available upon search (if full-genome sequence data is available) or upon further sequencing (since tag sites are unique enough to serve as primer annealing sites).
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PAR-CLIP
PAR-CLIP (photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation) is a biochemical method for identifying the binding sites of cellular RNA-binding proteins (RBPs) and microRNA-containing ribonucleoprotein complexes (miRNPs).
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Peak calling
Peak calling is a computational method used to identify areas in a genome that have been enriched with aligned reads as a consequence of performing a ChIP-sequencing or MeDIP-seq experiment.
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Phenotype
A phenotype is the composite of an organism's observable characteristics or traits, such as its morphology, development, biochemical or physiological properties, behavior, and products of behavior (such as a bird's nest).
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Polymerase
A polymerase is an enzyme (EC 2.7.7.6/7/19/48/49) that synthesizes long chains of polymers or nucleic acids.
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Post-translational modification
Post-translational modification (PTM) refers to the covalent and generally enzymatic modification of proteins following protein biosynthesis.
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Protein
Proteins are large biomolecules, or macromolecules, consisting of one or more long chains of amino acid residues.
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Protein microarray
A protein microarray (or protein chip) is a high-throughput method used to track the interactions and activities of proteins, and to determine their function, and determining function on a large scale.
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Pyrosequencing
Pyrosequencing is a method of DNA sequencing (determining the order of nucleotides in DNA) based on the "sequencing by synthesis" principle, in which the sequencing is performed by detecting the nucleotide incorporated by a DNA polymerase.
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RNA immunoprecipitation chip
A RNA immunoprecipitation chip (RIP-Chip) is immunoprecipitation of an RNA-binding protein coupled to reverse transcription and a microarray.
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Sono-Seq
Sono-Seq (Sonication of Cross-linked Chromatin Sequencing) is a method in molecular biology used for determining the sequences of those DNA regions in the genome near regions of open chromatin of expressed genes.
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Systematic evolution of ligands by exponential enrichment
Systematic evolution of ligands by exponential enrichment (SELEX), also referred to as in vitro selection or in vitro evolution, is a combinatorial chemistry technique in molecular biology for producing oligonucleotides of either single-stranded DNA or RNA that specifically bind to a target ligand or ligands.
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TCP-seq
Translation complex profile sequencing (TCP-seq) is a molecular biology method for obtaining snapshots of momentary distribution of protein synthesis complexes along messenger RNA (mRNA) chains.
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Tiling array
Tiling arrays are a subtype of microarray chips.
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Transcription (biology)
Transcription is the first step of gene expression, in which a particular segment of DNA is copied into RNA (especially mRNA) by the enzyme RNA polymerase.
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Transcription factor
In molecular biology, a transcription factor (TF) (or sequence-specific DNA-binding factor) is a protein that controls the rate of transcription of genetic information from DNA to messenger RNA, by binding to a specific DNA sequence.
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ChIP-Seq, ChIP-seq, ChiP-sequencing, Chip-Seq, Chip-Sequencing, Chip-sequencing, Chipseq.
References
[1] https://en.wikipedia.org/wiki/ChIP-sequencing
