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DNA shuffling

Index DNA shuffling

DNA shuffling is a way to rapidly propagate beneficial mutations in a directed evolution experiment. [1]

20 relations: Base pair, Chimera (genetics), Deoxyribonuclease, Directed evolution, DNA, DNA ligase, DNA polymerase, Enzyme, Gene, Genetic recombination, Homologous recombination, Homology (biology), Hybrid (biology), Library (biology), Mutation, Polymerase chain reaction, Primer (molecular biology), Random chimeragenesis on transient templates, Restriction enzyme, Structure-based combinatorial protein engineering.

Base pair

A base pair (bp) is a unit consisting of two nucleobases bound to each other by hydrogen bonds.

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Chimera (genetics)

A genetic chimerism or chimera (also spelled chimaera) is a single organism composed of cells with distinct genotypes.

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Deoxyribonuclease

A deoxyribonuclease (DNase, for short) is an enzyme that catalyzes the hydrolytic cleavage of phosphodiester linkages in the DNA backbone, thus degrading DNA.

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Directed evolution

Directed evolution (DE, "gelenkte Evolution") is a method used in protein engineering that mimics the process of natural selection to evolve proteins or nucleic acids toward a user-defined goal.

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DNA

Deoxyribonucleic acid (DNA) is a thread-like chain of nucleotides carrying the genetic instructions used in the growth, development, functioning and reproduction of all known living organisms and many viruses.

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DNA ligase

DNA ligase is a specific type of enzyme, a ligase, that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond.

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DNA polymerase

DNA polymerases are enzymes that synthesize DNA molecules from deoxyribonucleotides, the building blocks of DNA.

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Enzyme

Enzymes are macromolecular biological catalysts.

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Gene

In biology, a gene is a sequence of DNA or RNA that codes for a molecule that has a function.

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Genetic recombination

Genetic recombination (aka genetic reshuffling) is the production of offspring with combinations of traits that differ from those found in either parent.

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Homologous recombination

Homologous recombination is a type of genetic recombination in which nucleotide sequences are exchanged between two similar or identical molecules of DNA.

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Homology (biology)

In biology, homology is the existence of shared ancestry between a pair of structures, or genes, in different taxa.

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Hybrid (biology)

In biology, a hybrid, or crossbreed, is the result of combining the qualities of two organisms of different breeds, varieties, species or genera through sexual reproduction.

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Library (biology)

In molecular biology, a library is a collection of DNA fragments that is stored and propagated in a population of micro-organisms through the process of molecular cloning.

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Mutation

In biology, a mutation is the permanent alteration of the nucleotide sequence of the genome of an organism, virus, or extrachromosomal DNA or other genetic elements.

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Polymerase chain reaction

Polymerase chain reaction (PCR) is a technique used in molecular biology to amplify a single copy or a few copies of a segment of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence.

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Primer (molecular biology)

A primer is a short strand of RNA or DNA (generally about 18-22 bases) that serves as a starting point for DNA synthesis.

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Random chimeragenesis on transient templates

Random chimeragenesis on transient templates (RACHITT) is a method to perform molecular mutagenesis at a high recombination rate.

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Restriction enzyme

A restriction enzyme or restriction endonuclease is an enzyme that cleaves DNA into fragments at or near specific recognition sites within the molecule known as restriction sites.

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Structure-based combinatorial protein engineering

Structure-based combinatorial protein engineering (SCOPE) is a synthetic biology technique for creating gene libraries (lineages) of defined composition designed from structural and probabilistic constraints of the encoded proteins.

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Dna shuffling.

References

[1] https://en.wikipedia.org/wiki/DNA_shuffling

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