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Flow cytometry

Index Flow cytometry

In biotechnology, flow cytometry is a laser- or impedance-based, biophysical technology employed in cell counting, cell sorting, biomarker detection and protein engineering, by suspending cells in a stream of fluid and passing them through an electronic detection apparatus. [1]

132 relations: Absorption (electromagnetic radiation), Alternating current, Aneuploidy, Annexin A5 affinity assay, Antigen, Apoptosis, Bacterial display, Beckman Coulter, Becton Dickinson, Bioconductor, Biomarker, BIOS Scientific Publishers, Biotechnology, Botany, Calcium, Capacitance, Caspase, Cell (biology), Cell counting, Cell cycle analysis, Cell growth, Cell membrane, Cell sorting, Chemical substance, Chlorophyll, Clinical trial, Cluster of differentiation, Confocal microscopy, Copy-number variation, Coulter counter, Current Protocols, Cytogenetics, Cytokine, Cytometry, Deconvolution, Diameter, Dielectrophoresis, Dimension, Direct current, DNA, DNA fragmentation, DNA repair, Electric charge, Electrical impedance, Electrostatic deflection, ELISA, Emission spectrum, Endoreduplication, Enzyme, Excited state, ..., Fertility, Flow cytometry, Flow Cytometry Standard, Flow-FISH, FlowJo, Fluorescence, Fluorescent tag, Forward scatter, Göttingen, GenePattern, German language, Glutathione, Graz University of Technology, Green fluorescent protein, Hematology, Histogram, Immunoassay, Immunology, Intensity (physics), Intracellular, Ion laser, Ionization, JMP (statistical software), Kinetic energy, Kyoto Prize, Lab-on-a-chip, Lanthanide, Laser, Laser diode, Leonard Herzenberg, Linearity, Liquid, Logarithmic scale, Magnesium, Marine biology, Mass cytometry, Medical guideline, Medicine, Membrane fluidity, Membrane potential, Mercury (element), Microfluorimetry, Microscope, Molecular biology, Morphology (biology), Multiple drug resistance, Multivariate statistics, Neuroscience, Novus Biologicals, Oregon State University, Oscillation, Oxford University Press, Pathology, PH, Phenotype, Phycoerythrin, Pigment, Plankton, Plasma (physics), Ploidy, Protein, Protein engineering, Purdue University, Quantification (science), Quantum dot, Respiratory burst, RNA, Salk Institute for Biological Studies, Scattering, Science (journal), Sex selection, Smithsonian Institution Archives, Sperm sorting, Time-of-flight mass spectrometry, Tissue (biology), Trademark, Tumors of the hematopoietic and lymphoid tissues, University of Münster, Viability assay, Wallace H. Coulter, Xenon, Yeast display. Expand index (82 more) »

Absorption (electromagnetic radiation)

In physics, absorption of electromagnetic radiation is the way in which the energy of a photon is taken up by matter, typically the electrons of an atom.

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Alternating current

Alternating current (AC) is an electric current which periodically reverses direction, in contrast to direct current (DC) which flows only in one direction.

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Aneuploidy

Aneuploidy is the presence of an abnormal number of chromosomes in a cell, for example a human cell having 45 or 47 chromosomes instead of the usual 46.

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Annexin A5 affinity assay

In molecular biology, an annexin A5 affinity assay is a test to quantify the number of cells undergoing apoptosis.

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Antigen

In immunology, an antigen is a molecule capable of inducing an immune response (to produce an antibody) in the host organism.

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Apoptosis

Apoptosis (from Ancient Greek ἀπόπτωσις "falling off") is a process of programmed cell death that occurs in multicellular organisms.

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Bacterial display

Bacterial display (or bacteria display or bacterial surface display) is a protein engineering technique used for in vitro protein evolution.

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Beckman Coulter

Beckman Coulter Inc., is an American company that makes biomedical laboratory instruments.

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Becton Dickinson

Becton, Dickinson and Company (BD) is an American medical technology company that manufactures and sells medical devices, instrument systems, and reagents.

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Bioconductor

Bioconductor is a free, open source and open development software project for the analysis and comprehension of genomic data generated by wet lab experiments in molecular biology.

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Biomarker

A biomarker, or biological marker, generally refers to a measurable indicator of some biological state or condition.

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BIOS Scientific Publishers

BIOS Scientific Publishers was an English publisher.

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Biotechnology

Biotechnology is the broad area of science involving living systems and organisms to develop or make products, or "any technological application that uses biological systems, living organisms, or derivatives thereof, to make or modify products or processes for specific use" (UN Convention on Biological Diversity, Art. 2).

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Botany

Botany, also called plant science(s), plant biology or phytology, is the science of plant life and a branch of biology.

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Calcium

Calcium is a chemical element with symbol Ca and atomic number 20.

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Capacitance

Capacitance is the ratio of the change in an electric charge in a system to the corresponding change in its electric potential.

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Caspase

Caspases (cysteine-aspartic proteases, cysteine aspartases or cysteine-dependent aspartate-directed proteases) are a family of protease enzymes playing essential roles in programmed cell death (including apoptosis, pyroptosis and necroptosis) and inflammation.

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Cell (biology)

The cell (from Latin cella, meaning "small room") is the basic structural, functional, and biological unit of all known living organisms.

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Cell counting

Cell counting is any of various methods for the counting or similar quantification of cells in the life sciences, including medical diagnosis and treatment.

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Cell cycle analysis

Cell cycle analysis by DNA content measurement is a method that most frequently employs flow cytometry to distinguish cells in different phases of the cell cycle.

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Cell growth

The term cell growth is used in the contexts of biological cell development and cell division (reproduction).

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Cell membrane

The cell membrane (also known as the plasma membrane or cytoplasmic membrane, and historically referred to as the plasmalemma) is a biological membrane that separates the interior of all cells from the outside environment (the extracellular space).

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Cell sorting

Cell sorting is a method used to separate cells isolated from an organism's tissues according to their type.

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Chemical substance

A chemical substance, also known as a pure substance, is a form of matter that consists of molecules of the same composition and structure.

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Chlorophyll

Chlorophyll (also chlorophyl) is any of several related green pigments found in cyanobacteria and the chloroplasts of algae and plants.

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Clinical trial

Clinical trials are experiments or observations done in clinical research.

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Cluster of differentiation

The cluster of differentiation (also known as cluster of designation or classification determinant and often abbreviated as CD) is a protocol used for the identification and investigation of cell surface molecules providing targets for immunophenotyping of cells.

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Confocal microscopy

Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser confocal scanning microscopy (LCSM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation.

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Copy-number variation

Copy number variation (CNV) is a phenomenon in which sections of the genome are repeated and the number of repeats in the genome varies between individuals in the human population.

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Coulter counter

A Coulter counter is an apparatus for counting and sizing particles suspended in electrolytes.

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Current Protocols

Current Protocols is a series of laboratory manuals for life scientists.

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Cytogenetics

Cytogenetics is a branch of genetics that is concerned with how the chromosomes relate to cell behaviour, particularly to their behaviour during mitosis and meiosis.

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Cytokine

Cytokines are a broad and loose category of small proteins (~5–20 kDa) that are important in cell signaling.

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Cytometry

Cytometry is the measurement of the characteristics of cells.

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Deconvolution

In mathematics, deconvolution is an algorithm-based process used to reverse the effects of convolution on recorded data.

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Diameter

In geometry, a diameter of a circle is any straight line segment that passes through the center of the circle and whose endpoints lie on the circle.

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Dielectrophoresis

Dielectrophoresis (DEP) is a phenomenon in which a force is exerted on a dielectric particle when it is subjected to a non-uniform electric field.

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Dimension

In physics and mathematics, the dimension of a mathematical space (or object) is informally defined as the minimum number of coordinates needed to specify any point within it.

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Direct current

Direct current (DC) is the unidirectional flow of electric charge.

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DNA

Deoxyribonucleic acid (DNA) is a thread-like chain of nucleotides carrying the genetic instructions used in the growth, development, functioning and reproduction of all known living organisms and many viruses.

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DNA fragmentation

DNA fragmentation is the separation or breaking of DNA strands into pieces.

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DNA repair

DNA repair is a collection of processes by which a cell identifies and corrects damage to the DNA molecules that encode its genome.

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Electric charge

Electric charge is the physical property of matter that causes it to experience a force when placed in an electromagnetic field.

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Electrical impedance

Electrical impedance is the measure of the opposition that a circuit presents to a current when a voltage is applied.

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Electrostatic deflection

Electrostatic deflection refers to a technique for modifying the path of a beam of charged particles by the use of an electric field applied transverse to the path of the particles.

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ELISA

The enzyme-linked immunosorbent assay (ELISA) is a test that uses antibodies and color change to identify a substance.

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Emission spectrum

The emission spectrum of a chemical element or chemical compound is the spectrum of frequencies of electromagnetic radiation emitted due to an atom or molecule making a transition from a high energy state to a lower energy state.

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Endoreduplication

Endoreduplication (also referred to as endoreplication or endocycling) is replication of the nuclear genome in the absence of mitosis, which leads to elevated nuclear gene content and polyploidy.

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Enzyme

Enzymes are macromolecular biological catalysts.

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Excited state

In quantum mechanics, an excited state of a system (such as an atom, molecule or nucleus) is any quantum state of the system that has a higher energy than the ground state (that is, more energy than the absolute minimum).

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Fertility

Fertility is the natural capability to produce offspring.

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Flow cytometry

In biotechnology, flow cytometry is a laser- or impedance-based, biophysical technology employed in cell counting, cell sorting, biomarker detection and protein engineering, by suspending cells in a stream of fluid and passing them through an electronic detection apparatus.

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Flow Cytometry Standard

Flow Cytometry Standard (FCS) is a data file standard for the reading and writing of data from flow cytometry experiments.

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Flow-FISH

Flow-FISH (fluorescent in-situ hybridization) is a cytogenetic technique to quantify the copy number of specific repetitive elements in genomic DNA of whole cell populations via the combination of flow cytometry with cytogenetic fluorescent in situ hybridization staining protocols.

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FlowJo

FlowJo is a software package for analyzing flow cytometry data.

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Fluorescence

Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation.

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Fluorescent tag

In molecular biology and biotechnology, a fluorescent tag, also known as a label or probe, is a molecule that is attached chemically to aid in the labeling and detection of a biomolecule such as a protein, antibody, or amino acid.

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Forward scatter

In telecommunication and astronomy, forward scatter is the deflection—by diffraction, nonhomogeneous refraction, or nonspecular reflection by particulate matter of dimensions that are large with respect to the wavelength in question but small with respect to the beam diameter—of a portion of an incident electromagnetic wave, in such a manner that the energy so deflected propagates in a direction that is within 90° of the direction of propagation of the incident wave (i.e., the phase angle is greater than 90°).

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Göttingen

Göttingen (Low German: Chöttingen) is a university city in Lower Saxony, Germany.

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GenePattern

GenePattern is a freely available computational biology open-source software package originally created and developed at the Broad Institute for the analysis of genomic data.

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German language

German (Deutsch) is a West Germanic language that is mainly spoken in Central Europe.

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Glutathione

Glutathione (GSH) is an important antioxidant in plants, animals, fungi, and some bacteria and archaea.

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Graz University of Technology

Graz University of Technology (Technische Universität Graz, short TU Graz) is one of five universities in Styria, Austria.

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Green fluorescent protein

The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range.

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Hematology

Hematology, also spelled haematology, is the branch of medicine concerned with the study of the cause, prognosis, treatment, and prevention of diseases related to blood.

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Histogram

A histogram is an accurate representation of the distribution of numerical data.

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Immunoassay

An immunoassay is a biochemical test that measures the presence or concentration of a macromolecule or a small molecule in a solution through the use of an antibody (usually) or an antigen (sometimes).

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Immunology

Immunology is a branch of biology that covers the study of immune systems in all organisms.

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Intensity (physics)

In physics, intensity is the power transferred per unit area, where the area is measured on the plane perpendicular to the direction of propagation of the energy.

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Intracellular

In cell biology, molecular biology and related fields, the word intracellular means "inside the cell".

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Ion laser

An ion laser is a gas laser that uses an ionized gas as its lasing medium.

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Ionization

Ionization or ionisation, is the process by which an atom or a molecule acquires a negative or positive charge by gaining or losing electrons to form ions, often in conjunction with other chemical changes.

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JMP (statistical software)

JMP (pronounced "jump") is a suite of computer programs for statistical analysis developed by the JMP business unit of SAS Institute.

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Kinetic energy

In physics, the kinetic energy of an object is the energy that it possesses due to its motion.

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Kyoto Prize

The is Japan's highest private award for global achievement.

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Lab-on-a-chip

A lab-on-a-chip (LOC) is a device that integrates one or several laboratory functions on a single integrated circuit (commonly called a "chip") of only millimeters to a few square centimeters to achieve automation and high-throughput screening.

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Lanthanide

The lanthanide or lanthanoid series of chemical elements comprises the 15 metallic chemical elements with atomic numbers 57 through 71, from lanthanum through lutetium.

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Laser

A laser is a device that emits light through a process of optical amplification based on the stimulated emission of electromagnetic radiation.

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Laser diode

A laser diode, (LD), injection laser diode (ILD), or diode laser is a semiconductor device similar to a light-emitting diode in which the laser beam is created at the diode's junction.

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Leonard Herzenberg

Leonard Arthur "Len" Herzenberg (November 5, 1931 – October 27, 2013) was an immunologist, geneticist and professor at Stanford University.

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Linearity

Linearity is the property of a mathematical relationship or function which means that it can be graphically represented as a straight line.

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Liquid

A liquid is a nearly incompressible fluid that conforms to the shape of its container but retains a (nearly) constant volume independent of pressure.

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Logarithmic scale

A logarithmic scale is a nonlinear scale used when there is a large range of quantities.

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Magnesium

Magnesium is a chemical element with symbol Mg and atomic number 12.

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Marine biology

Marine biology is the scientific study of marine life, organisms in the sea.

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Mass cytometry

Mass cytometry is a mass spectrometry technique based on inductively coupled plasma mass spectrometry and time of flight mass spectrometry used for the determination of the properties of cells (cytometry).

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Medical guideline

A medical guideline (also called a clinical guideline or clinical practice line) is a document with the aim of guiding decisions and criteria regarding diagnosis, management, and treatment in specific areas of healthcare.

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Medicine

Medicine is the science and practice of the diagnosis, treatment, and prevention of disease.

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Membrane fluidity

In biology, membrane fluidity refers to the viscosity of the lipid bilayer of a cell membrane or a synthetic lipid membrane.

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Membrane potential

The term "membrane potential" may refer to one of three kinds of membrane potential.

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Mercury (element)

Mercury is a chemical element with symbol Hg and atomic number 80.

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Microfluorimetry

Microfluorimetry is an adaption of fluorimetry for studying the biochemical and biophysical properties of cells by using microscopy to image cell components tagged with fluorescent molecules.

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Microscope

A microscope (from the μικρός, mikrós, "small" and σκοπεῖν, skopeîn, "to look" or "see") is an instrument used to see objects that are too small to be seen by the naked eye.

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Molecular biology

Molecular biology is a branch of biology which concerns the molecular basis of biological activity between biomolecules in the various systems of a cell, including the interactions between DNA, RNA, proteins and their biosynthesis, as well as the regulation of these interactions.

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Morphology (biology)

Morphology is a branch of biology dealing with the study of the form and structure of organisms and their specific structural features.

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Multiple drug resistance

Multiple drug resistance (MDR), multidrug resistance or multiresistance is antimicrobial resistance shown by a species of microorganism to multiple antimicrobial drugs.

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Multivariate statistics

Multivariate statistics is a subdivision of statistics encompassing the simultaneous observation and analysis of more than one outcome variable.

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Neuroscience

Neuroscience (or neurobiology) is the scientific study of the nervous system.

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Novus Biologicals

Novus Biologicals was a biotech company based in Littleton, Colorado, USA with offices in San Diego, California, Oakville, ON, Canada and the Cambridge Science Park in Cambridge, UK.

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Oregon State University

Oregon State University (OSU) is an international, public research university in the northwest United States, located in Corvallis, Oregon.

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Oscillation

Oscillation is the repetitive variation, typically in time, of some measure about a central value (often a point of equilibrium) or between two or more different states.

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Oxford University Press

Oxford University Press (OUP) is the largest university press in the world, and the second oldest after Cambridge University Press.

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Pathology

Pathology (from the Ancient Greek roots of pathos (πάθος), meaning "experience" or "suffering" and -logia (-λογία), "study of") is a significant field in modern medical diagnosis and medical research, concerned mainly with the causal study of disease, whether caused by pathogens or non-infectious physiological disorder.

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PH

In chemistry, pH is a logarithmic scale used to specify the acidity or basicity of an aqueous solution.

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Phenotype

A phenotype is the composite of an organism's observable characteristics or traits, such as its morphology, development, biochemical or physiological properties, behavior, and products of behavior (such as a bird's nest).

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Phycoerythrin

Phycoerythrin (PE) is a red protein-pigment complex from the light-harvesting phycobiliprotein family, present in red algae and cryptophytes, accessory to the main chlorophyll pigments responsible for photosynthesis.

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Pigment

A pigment is a material that changes the color of reflected or transmitted light as the result of wavelength-selective absorption.

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Plankton

Plankton (singular plankter) are the diverse collection of organisms that live in large bodies of water and are unable to swim against a current.

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Plasma (physics)

Plasma (Henry George Liddell, Robert Scott, A Greek English Lexicon, on Perseus) is one of the four fundamental states of matter, and was first described by chemist Irving Langmuir in the 1920s.

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Ploidy

Ploidy is the number of complete sets of chromosomes in a cell, and hence the number of possible alleles for autosomal and pseudoautosomal genes.

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Protein

Proteins are large biomolecules, or macromolecules, consisting of one or more long chains of amino acid residues.

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Protein engineering

Protein engineering is the process of developing useful or valuable proteins.

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Purdue University

Purdue University is a public research university in West Lafayette, Indiana and is the flagship campus of the Purdue University system.

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Quantification (science)

In mathematics and empirical science, quantification (or quantitation) is the act of counting and measuring that maps human sense observations and experiences into quantities.

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Quantum dot

Quantum dots (QD) are very small semiconductor particles, only several nanometres in size, so small that their optical and electronic properties differ from those of larger particles.

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Respiratory burst

Respiratory burst (sometimes called oxidative burst) is the rapid release of reactive oxygen species (superoxide radical and hydrogen peroxide) from different types of cells.

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RNA

Ribonucleic acid (RNA) is a polymeric molecule essential in various biological roles in coding, decoding, regulation, and expression of genes.

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Salk Institute for Biological Studies

The Salk Institute for Biological Studies is an independent, non-profit, scientific research institute located in La Jolla, San Diego, California, United States.

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Scattering

Scattering is a general physical process where some forms of radiation, such as light, sound, or moving particles, are forced to deviate from a straight trajectory by one or more paths due to localized non-uniformities in the medium through which they pass.

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Science (journal)

Science, also widely referred to as Science Magazine, is the peer-reviewed academic journal of the American Association for the Advancement of Science (AAAS) and one of the world's top academic journals.

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Sex selection

Sex selection is the attempt to control the sex of the offspring to achieve a desired sex.

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Smithsonian Institution Archives

The Smithsonian Institution Archives (SIA) is the archives of the Smithsonian Institution.

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Sperm sorting

Sperm sorting is a means of choosing what type of sperm cell is to fertilize the egg cell.

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Time-of-flight mass spectrometry

Time-of-flight mass spectrometry (TOFMS) is a method of mass spectrometry in which an ion's mass-to-charge ratio is determined via a time of flight measurement.

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Tissue (biology)

In biology, tissue is a cellular organizational level between cells and a complete organ.

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Trademark

A trademark, trade mark, or trade-markThe styling of trademark as a single word is predominantly used in the United States and Philippines only, while the two-word styling trade mark is used in many other countries around the world, including the European Union and Commonwealth and ex-Commonwealth jurisdictions (although Canada officially uses "trade-mark" pursuant to the Trade-mark Act, "trade mark" and "trademark" are also commonly used).

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Tumors of the hematopoietic and lymphoid tissues

Tumors of the hematopoietic and lymphoid tissues or haematopoietic and lymphoid malignancies are tumors that affect the blood, bone marrow, lymph, and lymphatic system.

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University of Münster

The University of Münster (Westfälische Wilhelms-Universität Münster, WWU) is a public university located in the city of Münster, North Rhine-Westphalia in Germany.

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Viability assay

A viability assay is an assay to determine the ability of organs, cells or tissues to maintain or recover viability.

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Wallace H. Coulter

Wallace H. Coulter (February 17, 1913 – August 7, 1998) was an American electrical engineer, inventor, and businessman.

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Xenon

Xenon is a chemical element with symbol Xe and atomic number 54.

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Yeast display

Yeast display (or yeast surface display) is a protein engineering technique that uses the expression of recombinant proteins incorporated into the cell wall of yeast for isolating and engineering antibodies.

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Redirects here:

Cytofluorimeter, Cytometer, FAC sorting, FACScan, Flow cytometer, Flow immunophenotyping, Flow sorting, Fluorescence activated cell sorter, Fluorescence activated cell sorting, Fluorescence-activated cell sorter, Fluorescence-activated cell sorting, Fluorescent-activated cell sorting, Impedance flow cytometry.

References

[1] https://en.wikipedia.org/wiki/Flow_cytometry

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