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Isoelectric focusing

Index Isoelectric focusing

Isoelectric focusing (IEF), also known as electrofocusing, is a technique for separating different molecules by differences in their isoelectric point (pI). [1]

25 relations: Acid, Agarose, Aliphatic compound, Alzheimer's disease, Amino acid, Amphoterism, Anode, Base (chemistry), Capillary electrophoresis, Cathode, Electric current, Electrophoresis, Eukaryote, Gel electrophoresis, Immobilized pH gradient, Isoelectric point, Molecular mass, Molecule, PH, Phosphorylation, Polyacrylamide, Protein, SDS-PAGE, Starch, Two-dimensional gel electrophoresis.

Acid

An acid is a molecule or ion capable of donating a hydron (proton or hydrogen ion H+), or, alternatively, capable of forming a covalent bond with an electron pair (a Lewis acid).

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Agarose

Agarose is a polysaccharide, generally extracted from certain red seaweed.

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Aliphatic compound

In organic chemistry, hydrocarbons (compounds composed of carbon and hydrogen) are divided into two classes: aromatic compounds and aliphatic compounds (G. aleiphar, fat, oil) also known as non-aromatic compounds.

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Alzheimer's disease

Alzheimer's disease (AD), also referred to simply as Alzheimer's, is a chronic neurodegenerative disease that usually starts slowly and worsens over time.

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Amino acid

Amino acids are organic compounds containing amine (-NH2) and carboxyl (-COOH) functional groups, along with a side chain (R group) specific to each amino acid.

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Amphoterism

In chemistry, an amphoteric compound is a molecule or ion that can react both as an acid as well as a base.

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Anode

An anode is an electrode through which the conventional current enters into a polarized electrical device.

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Base (chemistry)

In chemistry, bases are substances that, in aqueous solution, release hydroxide (OH−) ions, are slippery to the touch, can taste bitter if an alkali, change the color of indicators (e.g., turn red litmus paper blue), react with acids to form salts, promote certain chemical reactions (base catalysis), accept protons from any proton donor, and/or contain completely or partially displaceable OH− ions.

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Capillary electrophoresis

Capillary electrophoresis (CE) is a family of electrokinetic separation methods performed in submillimeter diameter capillaries and in micro- and nanofluidic channels.

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Cathode

A cathode is the electrode from which a conventional current leaves a polarized electrical device.

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Electric current

An electric current is a flow of electric charge.

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Electrophoresis

Electrophoresis (from the Greek "Ηλεκτροφόρηση" meaning "to bear electrons") is the motion of dispersed particles relative to a fluid under the influence of a spatially uniform electric field.

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Eukaryote

Eukaryotes are organisms whose cells have a nucleus enclosed within membranes, unlike Prokaryotes (Bacteria and other Archaea).

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Gel electrophoresis

Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge.

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Immobilized pH gradient

Immobilized pH gradient (IPG) gels are the acrylamide gel matrix co-polymerized with the pH gradient, which result in completely stable gradients except the most alkaline (>12) pH values.

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Isoelectric point

The isoelectric point (pI, pH(I), IEP), is the pH at which a particular molecule carries no net electrical charge or is electrically neutral in the statistical mean.

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Molecular mass

Relative Molecular mass or molecular weight is the mass of a molecule.

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Molecule

A molecule is an electrically neutral group of two or more atoms held together by chemical bonds.

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PH

In chemistry, pH is a logarithmic scale used to specify the acidity or basicity of an aqueous solution.

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Phosphorylation

In chemistry, phosphorylation of a molecule is the attachment of a phosphoryl group.

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Polyacrylamide

Polyacrylamide (IUPAC poly(2-propenamide) or poly(1-carbamoylethylene), abbreviated as PAM) is a polymer (-CH2CHCONH2-) formed from acrylamide subunits.

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Protein

Proteins are large biomolecules, or macromolecules, consisting of one or more long chains of amino acid residues.

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SDS-PAGE

SDS-PAGE (sodium dodecyl sulfate–polyacrylamide gel electrophoresis) is a variant of polyacrylamide gel electrophoresis, an analytical method in biochemistry for the separation of charged molecules in mixtures by their molecular masses in an electric field.

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Starch

Starch or amylum is a polymeric carbohydrate consisting of a large number of glucose units joined by glycosidic bonds.

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Two-dimensional gel electrophoresis

Two-dimensional gel electrophoresis, abbreviated as 2-DE or 2-D electrophoresis, is a form of gel electrophoresis commonly used to analyze proteins.

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Redirects here:

Electrofocusing, Isoelectric focussing.

References

[1] https://en.wikipedia.org/wiki/Isoelectric_focusing

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