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Overlap extension polymerase chain reaction

Index Overlap extension polymerase chain reaction

The overlap extension polymerase chain reaction (or OE-PCR) is a variant of PCR. [1]

11 relations: Agarose gel electrophoresis, Asymmetric PCR, DNA replication, Indel, Mutation, Nucleic acid thermodynamics, Oligonucleotide, Polymerase chain reaction, Polynucleotide, Primer (molecular biology), Protein dimer.

Agarose gel electrophoresis

Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, genetics, and clinical chemistry to separate a mixed population of macromolecules such as DNA or proteins in a matrix of agarose, one of the two main components of agar.

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Asymmetric PCR

Asymmetric PCR is a variation of PCR used to preferentially amplify one strand of the original DNA more than the other.

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DNA replication

In molecular biology, DNA replication is the biological process of producing two identical replicas of DNA from one original DNA molecule.

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Indel

Indel is a molecular biology term for an '''in'''sertion or '''del'''etion of bases in the genome of an organism.

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Mutation

In biology, a mutation is the permanent alteration of the nucleotide sequence of the genome of an organism, virus, or extrachromosomal DNA or other genetic elements.

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Nucleic acid thermodynamics

Nucleic acid thermodynamics is the study of how temperature affects the nucleic acid structure of double-stranded DNA (dsDNA).

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Oligonucleotide

Oligonucleotides are short DNA or RNA molecules, oligomers, that have a wide range of applications in genetic testing, research, and forensics.

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Polymerase chain reaction

Polymerase chain reaction (PCR) is a technique used in molecular biology to amplify a single copy or a few copies of a segment of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence.

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Polynucleotide

A polynucleotide molecule is a biopolymer composed of 13 or more nucleotide monomers covalently bonded in a chain.

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Primer (molecular biology)

A primer is a short strand of RNA or DNA (generally about 18-22 bases) that serves as a starting point for DNA synthesis.

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Protein dimer

In biochemistry, a protein dimer is a macromolecular complex formed by two protein monomers, or single proteins, which are usually non-covalently bound.

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Redirects here:

OE PCR, OE-PCR, OEPCR, Oe PCR, Oe polymerase chain reaction, Oe-pcr, Oepcr, Overlap Extension Polymerase Chain Reaction, Overlap-extension PCR, Overlap-extension polymerase chain reaction, PCR overlap extension mutagenesis, SOEing PCR, Splicing by overlap extension polymerase chain reaction.

References

[1] https://en.wikipedia.org/wiki/Overlap_extension_polymerase_chain_reaction

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