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13 relations: Antimicrobial resistance, Bacteriophage, Blue–white screen, Cloning, DNA, Escherichia coli, Gene, Helper virus, Lac operon, Multiple cloning site, Origin of replication, Phagemid, X-gal.
Antimicrobial resistance
Antimicrobial resistance (AMR or AR) is the ability of a microbe to resist the effects of medication that once could successfully treat the microbe.
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Bacteriophage
A bacteriophage, also known informally as a phage, is a virus that infects and replicates within Bacteria and Archaea.
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Blue–white screen
The blue–white screen is a screening technique that allows for the rapid and convenient detection of recombinant bacteria in vector-based molecular cloning experiments.
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Cloning
Cloning is the process of producing genetically identical individuals of an organism either naturally or artificially.
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DNA
Deoxyribonucleic acid (DNA) is a thread-like chain of nucleotides carrying the genetic instructions used in the growth, development, functioning and reproduction of all known living organisms and many viruses.
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Escherichia coli
Escherichia coli (also known as E. coli) is a Gram-negative, facultatively anaerobic, rod-shaped, coliform bacterium of the genus Escherichia that is commonly found in the lower intestine of warm-blooded organisms (endotherms).
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Gene
In biology, a gene is a sequence of DNA or RNA that codes for a molecule that has a function.
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Helper virus
A helper virus is a virus used when producing copies of a helper dependent viral vector which does not have the ability to replicate on its own.
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Lac operon
The lac operon (lactose operon) is an operon required for the transport and metabolism of lactose in Escherichia coli and many other enteric bacteria.
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Multiple cloning site
A multiple cloning site (MCS), also called a polylinker, is a short segment of DNA which contains many (up to ~20) restriction sites - a standard feature of engineered plasmids.
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Origin of replication
The origin of replication (also called the replication origin) is a particular sequence in a genome at which replication is initiated.
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Phagemid
A phagemid or phasmid is a DNA-based cloning vector, which has both bacteriophage and plasmid properties.
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X-gal
X-gal (also abbreviated BCIG for 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside) is an organic compound consisting of galactose linked to a substituted indole.
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