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49 relations: Adenine, Amino acid, Beacon designer, Biochemistry, BLAST, Cytosine, De novo synthesis, DECIPHER (software), Deoxyribonucleotide, Directionality (molecular biology), DNA, DNA ligase, DNA polymerase, DNA polymerase I, DNA polymerase III holoenzyme, DNA replication, DNA sequencing, Enzyme, Eukaryote, Flap structure-specific endonuclease 1, GenBank, Gene, Genetic code, HIV, Hydroxy group, In silico PCR, Isoleucine, Molecular biology, National Center for Biotechnology Information, Nucleic acid hybridization, Nucleic acid notation, Nucleotide, Okazaki fragments, Oligonucleotide, Oligonucleotide synthesis, Organism, Polymerase chain reaction, Primase, Prokaryote, Protein primary structure, Replication protein A, Reverse transcriptase, RNA, Sanger sequencing, Sense (molecular biology), TA cloning, Thymine, Touchdown polymerase chain reaction, Upstream and downstream (DNA).
Adenine
Adenine (A, Ade) is a nucleobase (a purine derivative).
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Amino acid
Amino acids are organic compounds containing amine (-NH2) and carboxyl (-COOH) functional groups, along with a side chain (R group) specific to each amino acid.
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Beacon designer
Beacon Designer designs highly specific and efficient primers and probes for real time PCR (polymerase chain reaction) assays.
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Biochemistry
Biochemistry, sometimes called biological chemistry, is the study of chemical processes within and relating to living organisms.
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BLAST
In bioinformatics, BLAST for Basic Local Alignment Search Tool is an algorithm for comparing primary biological sequence information, such as the amino-acid sequences of proteins or the nucleotides of DNA sequences.
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Cytosine
Cytosine (C) is one of the four main bases found in DNA and RNA, along with adenine, guanine, and thymine (uracil in RNA).
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De novo synthesis
De novo synthesis refers to the synthesis of complex molecules from simple molecules such as sugars or amino acids, as opposed to recycling after partial degradation.
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DECIPHER (software)
Decipher, stylized as DECIPHER, is a software toolset that can be used to decipher and manage biological sequences efficiently using the programming language R. Some functions of the program are accessible online through web tools.
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Deoxyribonucleotide
A deoxyribonucleotide is the monomer, or single unit, of DNA, or deoxyribonucleic acid.
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Directionality (molecular biology)
Directionality, in molecular biology and biochemistry, is the end-to-end chemical orientation of a single strand of nucleic acid.
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DNA
Deoxyribonucleic acid (DNA) is a thread-like chain of nucleotides carrying the genetic instructions used in the growth, development, functioning and reproduction of all known living organisms and many viruses.
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DNA ligase
DNA ligase is a specific type of enzyme, a ligase, that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond.
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DNA polymerase
DNA polymerases are enzymes that synthesize DNA molecules from deoxyribonucleotides, the building blocks of DNA.
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DNA polymerase I
DNA polymerase I (or Pol I) is an enzyme that participates in the process of prokaryotic DNA replication.
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DNA polymerase III holoenzyme
DNA polymerase III holoenzyme is the primary enzyme complex involved in prokaryotic DNA replication.
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DNA replication
In molecular biology, DNA replication is the biological process of producing two identical replicas of DNA from one original DNA molecule.
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DNA sequencing
DNA sequencing is the process of determining the precise order of nucleotides within a DNA molecule.
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Enzyme
Enzymes are macromolecular biological catalysts.
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Eukaryote
Eukaryotes are organisms whose cells have a nucleus enclosed within membranes, unlike Prokaryotes (Bacteria and other Archaea).
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Flap structure-specific endonuclease 1
Flap endonuclease 1 is an enzyme that in humans is encoded by the FEN1 gene.
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GenBank
The GenBank sequence database is an open access, annotated collection of all publicly available nucleotide sequences and their protein translations.
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Gene
In biology, a gene is a sequence of DNA or RNA that codes for a molecule that has a function.
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Genetic code
The genetic code is the set of rules used by living cells to translate information encoded within genetic material (DNA or mRNA sequences) into proteins.
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HIV
The human immunodeficiency virus (HIV) is a lentivirus (a subgroup of retrovirus) that causes HIV infection and over time acquired immunodeficiency syndrome (AIDS).
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Hydroxy group
A hydroxy or hydroxyl group is the entity with the formula OH.
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In silico PCR
In silico PCR refers to computational tools used to calculate theoretical polymerase chain reaction (PCR) results using a given set of primers (probes) to amplify DNA sequences from a sequenced genome or transcriptome.
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Isoleucine
Isoleucine (symbol Ile or I) is an α-amino acid that is used in the biosynthesis of proteins.
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Molecular biology
Molecular biology is a branch of biology which concerns the molecular basis of biological activity between biomolecules in the various systems of a cell, including the interactions between DNA, RNA, proteins and their biosynthesis, as well as the regulation of these interactions.
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National Center for Biotechnology Information
The National Center for Biotechnology Information (NCBI) is part of the United States National Library of Medicine (NLM), a branch of the National Institutes of Health (NIH).
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Nucleic acid hybridization
In molecular biology, hybridization (or hybridisation) is a phenomenon in which single-stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) molecules anneal to complementary DNA or RNA.
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Nucleic acid notation
The nucleic acid notation currently in use was first formalized by the International Union of Pure and Applied Chemistry (IUPAC) in 1970.
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Nucleotide
Nucleotides are organic molecules that serve as the monomer units for forming the nucleic acid polymers deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), both of which are essential biomolecules within all life-forms on Earth.
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Okazaki fragments
Okazaki fragments are short, newly synthesized DNA fragments that are formed on the lagging template strand during DNA replication.
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Oligonucleotide
Oligonucleotides are short DNA or RNA molecules, oligomers, that have a wide range of applications in genetic testing, research, and forensics.
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Oligonucleotide synthesis
Oligonucleotide synthesis is the chemical synthesis of relatively short fragments of nucleic acids with defined chemical structure (sequence).
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Organism
In biology, an organism (from Greek: ὀργανισμός, organismos) is any individual entity that exhibits the properties of life.
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Polymerase chain reaction
Polymerase chain reaction (PCR) is a technique used in molecular biology to amplify a single copy or a few copies of a segment of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence.
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Primase
DNA primase is an enzyme involved in the replication of DNA and is a type of RNA polymerase.
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Prokaryote
A prokaryote is a unicellular organism that lacks a membrane-bound nucleus, mitochondria, or any other membrane-bound organelle.
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Protein primary structure
Protein primary structure is the linear sequence of amino acids in a peptide or protein.
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Replication protein A
Replication protein A (RPA) is the major protein that binds to single-stranded DNA (ssDNA) in eukaryotic cells.
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Reverse transcriptase
A reverse transcriptase (RT) is an enzyme used to generate complementary DNA (cDNA) from an RNA template, a process termed reverse transcription.
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RNA
Ribonucleic acid (RNA) is a polymeric molecule essential in various biological roles in coding, decoding, regulation, and expression of genes.
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Sanger sequencing
Sanger sequencing is a method of DNA sequencing first commercialized by Applied Biosystems, based on the selective incorporation of chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA replication.
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Sense (molecular biology)
In molecular biology and genetics, the sense of nucleic acid molecules (often DNA or RNA) is the nature of their roles and their complementary molecules' nucleic acid units' roles in specifying amino acids.
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TA cloning
TA cloning (also known as rapid cloning or T cloning) is a subcloning technique that avoids the use of restriction enzymes and is easier and quicker than traditional subcloning.
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Thymine
---> Thymine (T, Thy) is one of the four nucleobases in the nucleic acid of DNA that are represented by the letters G–C–A–T.
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Touchdown polymerase chain reaction
The touchdown polymerase chain reaction or touchdown style polymerase chain reaction is a method of polymerase chain reaction by which primers avoid amplifying nonspecific sequences.
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Upstream and downstream (DNA)
In molecular biology and genetics, upstream and downstream both refer to relative positions of genetic code in DNA or RNA.
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DNA primer, Degenerate primer, PCR Primer, PCR primer, PCR-Primer, Primer (dna), Primer (genetics), Primer Design, Primer RNA, Primer removal, RNA primer, RNA primers, Rna primer, Universal primer, Universal primers.
References
[1] https://en.wikipedia.org/wiki/Primer_(molecular_biology)
