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Sequencing by ligation

Index Sequencing by ligation

Sequencing by ligation is a DNA sequencing method that uses the enzyme DNA ligase to identify the nucleotide present at a given position in a DNA sequence. [1]

13 relations: ABI Solid Sequencing, BMC Systems Biology, DNA ligase, DNA sequencing, Enzyme, Ligation (molecular biology), Nucleotide, Oligonucleotide, Phosphate, Polony sequencing, Restriction enzyme, Sequencing by hybridization, 2 base encoding.

ABI Solid Sequencing

SOLiD (Sequencing by Oligonucleotide Ligation and Detection) is a next-generation DNA sequencing technology developed by Life Technologies and has been commercially available since 2006.

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BMC Systems Biology

BMC Systems Biology is an open access peer-reviewed scientific journal that covers research in systems biology.

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DNA ligase

DNA ligase is a specific type of enzyme, a ligase, that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond.

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DNA sequencing

DNA sequencing is the process of determining the precise order of nucleotides within a DNA molecule.

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Enzyme

Enzymes are macromolecular biological catalysts.

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Ligation (molecular biology)

In molecular biology, ligation is the joining of two nucleic acid fragments through the action of an enzyme.

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Nucleotide

Nucleotides are organic molecules that serve as the monomer units for forming the nucleic acid polymers deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), both of which are essential biomolecules within all life-forms on Earth.

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Oligonucleotide

Oligonucleotides are short DNA or RNA molecules, oligomers, that have a wide range of applications in genetic testing, research, and forensics.

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Phosphate

A phosphate is chemical derivative of phosphoric acid.

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Polony sequencing

Polony sequencing is an inexpensive but highly accurate multiplex sequencing technique that can be used to “read” millions of immobilized DNA sequences in parallel.

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Restriction enzyme

A restriction enzyme or restriction endonuclease is an enzyme that cleaves DNA into fragments at or near specific recognition sites within the molecule known as restriction sites.

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Sequencing by hybridization

Sequencing by hybridization is a class of methods for determining the order in which nucleotides occur on a strand of DNA.

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2 base encoding

2 Base Encoding, also called SOLiD (sequencing by oligonucleotide ligation and detection), is a next-generation sequencing technology developed by Applied Biosystems and has been commercially available since 2008.

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DNA sequencing by ligation.

References

[1] https://en.wikipedia.org/wiki/Sequencing_by_ligation

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