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Two-dimensional gel electrophoresis

Two-dimensional gel electrophoresis, abbreviated as 2-DE or 2-D electrophoresis, is a form of gel electrophoresis commonly used to analyze proteins. [1]

25 relations: BioNumerics, Cell (biology), Chloroquine, Coomassie Brilliant Blue, Difference gel electrophoresis, DNA, DNA supercoil, Electrophoresis, Ethidium bromide, Gel electrophoresis, Immobilized pH gradient, In-gel digestion, Isoelectric focusing, Isoelectric point, Mass, Missing data, Multiprotein complex, Organelle, Polyacrylamide gel electrophoresis, Protein mass spectrometry, Protomap (proteomics), QPNC-PAGE, Quantitative proteomics, Silver stain, Sodium dodecyl sulfate.

BioNumerics

BioNumerics is a suite of bioinformatics software applications developed by the company Applied Maths NV.

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Cell (biology)

The cell (from Latin cella, meaning "small room") is the basic structural, functional, and biological unit of all known living organisms.

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Chloroquine

Chloroquine is a 4-aminoquinoline drug used in the treatment or prevention of malaria.

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Coomassie Brilliant Blue

Coomassie Brilliant Blue is the name of two similar triphenylmethane dyes that were developed for use in the textile industry but are now commonly used for staining proteins in analytical biochemistry.

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Difference gel electrophoresis

Difference gel electrophoresis (DIGE) is a form of gel electrophoresis where up to three different protein samples can be labeled with size-matched, charge-matched spectrally resolvable fluorescent dyes (for example Cy3, Cy5, Cy2) prior to two-dimensional electrophoresis.

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DNA

Deoxyribonucleic acid (DNA) is a molecule that carries most of the genetic instructions used in the development, functioning and reproduction of all known living organisms and many viruses.

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DNA supercoil

DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand.

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Electrophoresis

Electrophoresis is the motion of dispersed particles relative to a fluid under the influence of a spatially uniform electric field.

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Ethidium bromide

Ethidium bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis.

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Gel electrophoresis

Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge.

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Immobilized pH gradient

Immobilized pH gradient (IPG) gels are the acrylamide gel matrix co-polymerized with the pH gradient, which result in completely stable gradients except the most alkaline (>12) pH values.

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In-gel digestion

The in-gel digestion is part of the sample preparation for the mass spectrometric identification of proteins in course of proteomic analysis.

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Isoelectric focusing

Isoelectric focusing (IEF), also known as electrofocusing, is a technique for separating different molecules by differences in their isoelectric point (pI).

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Isoelectric point

The isoelectric point (pI, pH(I), IEP), is the pH at which a particular molecule carries no net electrical charge.

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Mass

In physics, mass is a property of a physical body which determines the strength of its mutual gravitational attraction to other bodies, its resistance to being accelerated by a force, and in the theory of relativity gives the mass–energy content of a system.

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Missing data

In statistics, missing data, or missing values, occur when no data value is stored for the variable in an observation.

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Multiprotein complex

A multiprotein complex (or protein complex) is a group of two or more associated polypeptide chains.

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Organelle

In cell biology, an organelle is a specialized subunit within a cell that has a specific function.

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Polyacrylamide gel electrophoresis

Polyacrylamide gel electrophoresis (PAGE), describes a technique widely used in biochemistry, forensics, genetics, molecular biology and biotechnology to separate biological macromolecules, usually proteins or nucleic acids, according to their electrophoretic mobility.

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Protein mass spectrometry

Protein mass spectrometry refers to the application of mass spectrometry to the study of proteins.

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Protomap (proteomics)

PROTOMAP is a recently developed proteomic technology for identifying changes to proteins that manifest in altered migration by one-dimensional SDS-PAGE.

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QPNC-PAGE

QPNC-PAGE, or quantitative preparative native continuous polyacrylamide gel electrophoresis, is a high-resolution technique applied in biochemistry and bioinorganic chemistry to separate proteins by isoelectric point.

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Quantitative proteomics

Quantitative proteomics is an analytical chemistry technique for determining the amount of proteins in a sample.

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Silver stain

Silver staining is the use of silver to selectively alter the appearance of a target in microscopy of histological sections; in temperature gradient gel electrophoresis; and in polyacrylamide gels.

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Sodium dodecyl sulfate

Sodium dodecyl sulfate (SDS or NaDS), sodium laurilsulfate or sodium lauryl sulfate (SLS) is an organic compound with the formula CH3(CH2)11SO4Na.

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References

[1] https://en.wikipedia.org/wiki/Two-dimensional_gel_electrophoresis

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