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Size-exclusion chromatography

Index Size-exclusion chromatography

Size-exclusion chromatography (SEC), also known as molecular sieve chromatography, is a chromatographic method in which molecules in solution are separated by their size, and in some cases molecular weight. [1]

40 relations: Adsorption, Aqueous normal-phase chromatography, Biomolecular structure, Biomolecule, Calibration curve, Chromatography, Dispersity, Dow Chemical Company, Dynamic light scattering, Elution, Fractionation, Friction, Gel electrophoresis, Gel permeation chromatography, High-performance liquid chromatography, Hydrodynamic radius, Ion chromatography, Jerker Porath, List of synthetic polymers, Logarithm, Low-angle laser light scattering, Macromolecule, Micellar liquid chromatography, Molar mass distribution, Molecular mass, Molecule, Multiangle light scattering, Normal distribution, PEGylation, Polymer, Polymer characterization, Polymer fractionation, Polystyrene, Radius of gyration, Refractive index, Solution, Spectroscopy, Stokes radius, Tetrahydrofuran, Viscometer.

Adsorption

Adsorption is the adhesion of atoms, ions or molecules from a gas, liquid or dissolved solid to a surface.

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Aqueous normal-phase chromatography

Aqueous normal-phase chromatography (ANP) is a chromatographic technique which involves the mobile phase region between reversed-phase chromatography (RP) and organic normal-phase chromatography (ONP).

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Biomolecular structure

Biomolecular structure is the intricate folded, three-dimensional shape that is formed by a molecule of protein, DNA, or RNA, and that is important to its function.

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Biomolecule

A biomolecule or biological molecule is a loosely used term for molecules and ions that are present in organisms, essential to some typically biological process such as cell division, morphogenesis, or development.

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Calibration curve

In analytical chemistry, a calibration curve, also known as a standard curve, is a general method for determining the concentration of a substance in an unknown sample by comparing the unknown to a set of standard samples of known concentration.

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Chromatography

Chromatography is a laboratory technique for the separation of a mixture.

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Dispersity

A monodisperse, or uniform, polymer is composed of molecules of the same mass.

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Dow Chemical Company

The Dow Chemical Company, commonly referred to as Dow, is an American multinational chemical corporation headquartered in Midland, Michigan, United States, and the predecessor of the merged company DowDuPont.

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Dynamic light scattering

Dynamic light scattering (DLS) is a technique in physics that can be used to determine the size distribution profile of small particles in suspension or polymers in solution.

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Elution

In analytical and organic chemistry, elution is the process of extracting one material from another by washing with a solvent; as in washing of loaded ion-exchange resins to remove captured ions.

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Fractionation

Fractionation is a separation process in which a certain quantity of a mixture (gas, solid, liquid, enzymes, suspension, or isotope) is divided during a phase transition, into a number of smaller quantities (fractions) in which the composition varies according to a gradient.

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Friction

Friction is the force resisting the relative motion of solid surfaces, fluid layers, and material elements sliding against each other.

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Gel electrophoresis

Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge.

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Gel permeation chromatography

Gel permeation chromatography (GPC) is a type of size exclusion chromatography (SEC), that separates analytes on the basis of size.

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High-performance liquid chromatography

High-performance liquid chromatography (HPLC; formerly referred to as high-pressure liquid chromatography), is a technique in analytical chemistry used to separate, identify, and quantify each component in a mixture.

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Hydrodynamic radius

The hydrodynamic radius of a macromolecule or colloid particle is R_.

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Ion chromatography

Ion chromatography (or ion-exchange chromatography) is a chromatography process that separates ions and polar molecules based on their affinity to the ion exchanger.

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Jerker Porath

Jerker Porath, (23 October 1921 – 21 January 2016) was a Swedish biochemist who invented several separation methods for biomolecules.

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List of synthetic polymers

Synthetic polymers are human-made polymers.

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Logarithm

In mathematics, the logarithm is the inverse function to exponentiation.

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Low-angle laser light scattering

Low-angle laser light scattering or LALLS is an application of light scattering that is particularly useful in conjunction with the technique of Size exclusion chromatography, one of the most powerful and widely used techniques to study the molecular mass distribution of a polymer.

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Macromolecule

A macromolecule is a very large molecule, such as protein, commonly created by the polymerization of smaller subunits (monomers).

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Micellar liquid chromatography

Micellar liquid chromatography (MLC) is a form of reversed phase liquid chromatography that uses an aqueous micellar solutions as the mobile phase.

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Molar mass distribution

In linear polymers the individual polymer chains rarely have exactly the same degree of polymerization and molar mass, and there is always a distribution around an average value.

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Molecular mass

Relative Molecular mass or molecular weight is the mass of a molecule.

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Molecule

A molecule is an electrically neutral group of two or more atoms held together by chemical bonds.

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Multiangle light scattering

Multiangle light scattering (MALS) describes a technique for measuring the light scattered by a sample into a plurality of angles.

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Normal distribution

In probability theory, the normal (or Gaussian or Gauss or Laplace–Gauss) distribution is a very common continuous probability distribution.

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PEGylation

PEGylation (often styled pegylation) is the process of both covalent and non-covalent attachment or amalgamation of polyethylene glycol (PEG, in pharmacy called macrogol) polymer chains to molecules and macrostructures, such as a drug, therapeutic protein or vesicle, which is then described as PEGylated (pegylated).

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Polymer

A polymer (Greek poly-, "many" + -mer, "part") is a large molecule, or macromolecule, composed of many repeated subunits.

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Polymer characterization

Polymer characterization is the analytical branch of polymer science.

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Polymer fractionation

Polymers are chainlike molecules that are made of the same repetition unit.

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Polystyrene

Polystyrene (PS) is a synthetic aromatic hydrocarbon polymer made from the monomer styrene.

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Radius of gyration

Radius of gyration or gyradius of a body about an axis of rotation is defined as the radial distance of a point from the axis of rotation at which, if whole mass of the body is assumed to be concentrated, its moment of inertia about the given axis would be the same as with its actual distribution of mass.

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Refractive index

In optics, the refractive index or index of refraction of a material is a dimensionless number that describes how light propagates through that medium.

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Solution

In chemistry, a solution is a special type of homogeneous mixture composed of two or more substances.

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Spectroscopy

Spectroscopy is the study of the interaction between matter and electromagnetic radiation.

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Stokes radius

The Stokes radius or Stokes-Einstein radius of a solute is the radius of a hard sphere that diffuses at the same rate as that solute.

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Tetrahydrofuran

Tetrahydrofuran (THF) is an organic compound with the formula (CH2)4O.

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Viscometer

A viscometer (also called viscosimeter) is an instrument used to measure the viscosity of a fluid.

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References

[1] https://en.wikipedia.org/wiki/Size-exclusion_chromatography

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