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Ethidium bromide

Index Ethidium bromide

Ethidium bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis. [1]

46 relations: Absorption spectroscopy, Acridine, Agarose gel electrophoresis, Ames test, Antimicrobial resistance, Aromaticity, Base pair, Bromoethane, Chemical compound, Dimethyl sulfoxide, DNA, DNA replication, Excited state, Fluorescence, Fluorescent tag, Gel electrophoresis, Gel electrophoresis of nucleic acids, GelGreen, GelRed, Heterocyclic compound, Hydrophobe, Intercalation (biochemistry), Isometamidium chloride, Kinetoplastida, Mitochondrial DNA, Molecular biology, Mutagen, Nucleic acid, Phenanthridine, Phenyl group, Polymerase chain reaction, Propidium monoazide, Quenching (fluorescence), Restriction digest, RNA, Safety data sheet, Sodium hypochlorite, Staining, SYBR Green I, Transcription (biology), Trypanosoma, Trypanosomatida, Trypanosomiasis, Ultraviolet, YEPD, Z-DNA.

Absorption spectroscopy

Absorption spectroscopy refers to spectroscopic techniques that measure the absorption of radiation, as a function of frequency or wavelength, due to its interaction with a sample.

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Acridine

Acridine is an organic compound and a nitrogen heterocycle with the formula C13H9N.

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Agarose gel electrophoresis

Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, genetics, and clinical chemistry to separate a mixed population of macromolecules such as DNA or proteins in a matrix of agarose, one of the two main components of agar.

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Ames test

The Ames test is a widely employed method that uses bacteria to test whether a given chemical can cause mutations in the DNA of the test organism.

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Antimicrobial resistance

Antimicrobial resistance (AMR or AR) is the ability of a microbe to resist the effects of medication that once could successfully treat the microbe.

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Aromaticity

In organic chemistry, the term aromaticity is used to describe a cyclic (ring-shaped), planar (flat) molecule with a ring of resonance bonds that exhibits more stability than other geometric or connective arrangements with the same set of atoms.

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Base pair

A base pair (bp) is a unit consisting of two nucleobases bound to each other by hydrogen bonds.

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Bromoethane

Bromoethane, also known as ethyl bromide, is a chemical compound of the haloalkanes group.

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Chemical compound

A chemical compound is a chemical substance composed of many identical molecules (or molecular entities) composed of atoms from more than one element held together by chemical bonds.

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Dimethyl sulfoxide

Dimethyl sulfoxide (DMSO) is an organosulfur compound with the formula (CH3)2SO.

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DNA

Deoxyribonucleic acid (DNA) is a thread-like chain of nucleotides carrying the genetic instructions used in the growth, development, functioning and reproduction of all known living organisms and many viruses.

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DNA replication

In molecular biology, DNA replication is the biological process of producing two identical replicas of DNA from one original DNA molecule.

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Excited state

In quantum mechanics, an excited state of a system (such as an atom, molecule or nucleus) is any quantum state of the system that has a higher energy than the ground state (that is, more energy than the absolute minimum).

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Fluorescence

Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation.

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Fluorescent tag

In molecular biology and biotechnology, a fluorescent tag, also known as a label or probe, is a molecule that is attached chemically to aid in the labeling and detection of a biomolecule such as a protein, antibody, or amino acid.

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Gel electrophoresis

Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge.

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Gel electrophoresis of nucleic acids

Nucleic acid electrophoresis is an analytical technique used to separate DNA or RNA fragments by size and reactivity.

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GelGreen

GelGreen is an intercalating nucleic acid stain used in molecular biology for agarose gel electrophoresis.

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GelRed

GelRed is an intercalating nucleic acid stain used in molecular biology for agarose gel electrophoresis.

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Heterocyclic compound

A heterocyclic compound or ring structure is a cyclic compound that has atoms of at least two different elements as members of its ring(s).

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Hydrophobe

In chemistry, hydrophobicity is the physical property of a molecule (known as a hydrophobe) that is seemingly repelled from a mass of water.

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Intercalation (biochemistry)

In biochemistry, intercalation is the insertion of molecules between the planar bases of deoxyribonucleic acid (DNA).

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Isometamidium chloride

Isometamidium chloride is a trypanocidal agent used in veterinary medicine.

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Kinetoplastida

Kinetoplastida (or Kinetoplastea, as a class) is a group of flagellated protists belonging to the phylum Euglenozoa, and characterised by the presence of an organelle with a large massed DNA called kinetoplast (hence the name).

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Mitochondrial DNA

Mitochondrial DNA (mtDNA or mDNA) is the DNA located in mitochondria, cellular organelles within eukaryotic cells that convert chemical energy from food into a form that cells can use, adenosine triphosphate (ATP).

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Molecular biology

Molecular biology is a branch of biology which concerns the molecular basis of biological activity between biomolecules in the various systems of a cell, including the interactions between DNA, RNA, proteins and their biosynthesis, as well as the regulation of these interactions.

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Mutagen

In genetics, a mutagen is a physical or chemical agent that changes the genetic material, usually DNA, of an organism and thus increases the frequency of mutations above the natural background level.

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Nucleic acid

Nucleic acids are biopolymers, or small biomolecules, essential to all known forms of life.

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Phenanthridine

Phenanthridine is a nitrogen heterocyclic compound that is the basis of DNA-binding fluorescent dyes through intercalation.

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Phenyl group

In organic chemistry, the phenyl group or phenyl ring is a cyclic group of atoms with the formula C6H5.

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Polymerase chain reaction

Polymerase chain reaction (PCR) is a technique used in molecular biology to amplify a single copy or a few copies of a segment of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence.

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Propidium monoazide

Propidium monoazide (PMA) is a photoreactive DNA-binding dye that preferentially binds to dsDNA.

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Quenching (fluorescence)

Quenching refers to any process which decreases the fluorescence intensity of a given substance.

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Restriction digest

A restriction digest is a procedure used in molecular biology to prepare DNA for analysis or other processing.

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RNA

Ribonucleic acid (RNA) is a polymeric molecule essential in various biological roles in coding, decoding, regulation, and expression of genes.

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Safety data sheet

A safety data sheet (SDS), material safety data sheet (MSDS), or product safety data sheet (PSDS) is an important component of product stewardship, occupational safety and health, and spill-handling procedures.

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Sodium hypochlorite

No description.

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Staining

Staining is an auxiliary technique used in microscopy to enhance contrast in the microscopic image.

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SYBR Green I

SYBR Green I (SG) is an asymmetrical cyanine dye used as a nucleic acid stain in molecular biology.

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Transcription (biology)

Transcription is the first step of gene expression, in which a particular segment of DNA is copied into RNA (especially mRNA) by the enzyme RNA polymerase.

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Trypanosoma

Trypanosoma is a genus of kinetoplastids (class Kinetoplastida), a monophyletic group of unicellular parasitic flagellate protozoa.

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Trypanosomatida

Trypanosomatida is a group of kinetoplastid excavates distinguished by having only a single flagellum.

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Trypanosomiasis

Trypanosomiasis or trypanosomosis is the name of several diseases in vertebrates caused by parasitic protozoan trypanosomes of the genus Trypanosoma.

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Ultraviolet

Ultraviolet (UV) is electromagnetic radiation with a wavelength from 10 nm to 400 nm, shorter than that of visible light but longer than X-rays.

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YEPD

YEPD or yeast extract peptone dextrose, also often abbreviated as YPD, is a complete medium for yeast growth.

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Z-DNA

Z-DNA is one of the many possible double helical structures of DNA.

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Redirects here:

2,7-diamino-10-ethyl-6-phenylphenanthridinium bromide, 2,7-diamino-10-ethyl-9-phenylphenanthridinium bromide, 3,8-diamino-1-ethyl-6-phenylphenantridinium bromide, 5-ethyl-6-phenyl-phenanthridine-3,8-diamine bromide, ATCvet code QP51AX06, C21H20BrN3, Ethidium, Ethidium Bromide, Homidium, Homidium bromide.

References

[1] https://en.wikipedia.org/wiki/Ethidium_bromide

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