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4Pi microscope

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A 4Pi microscope is a laser scanning fluorescence microscope with an improved axial resolution. [1]

20 relations: Beam splitter, Christoph Cremer, Confocal microscopy, Dichroic filter, Fluorescence microscope, Holography, Leica Microsystems, Mitochondrion, Multifocal plane microscopy, Objective (optics), Optical axis, Optical path length, Optical resolution, Point spread function, RESOLFT, Solid angle, STED microscopy, Stefan Hell, Thomas Cremer, Two-photon excitation microscopy.

Beam splitter

A beam splitter is an optical device that splits a beam of light in two.

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Christoph Cremer

Christoph Cremer (born in Freiburg im Breisgau, Germany) is a German physicist and professor at the Ruprecht-Karls-University Heidelberg, honorary professor at the University of Mainz and group leader at the Institute of Molecular Biology (IMB) a newly established research centre on the campus of the Johannes Gutenberg University of Mainz, Germany, who has successfully overcome the conventional limit of resolution that applies to light based investigations (the Abbe limit) by a range of different methods (1971/1978 development of the concept of 4Pi-microscopy; 1996 localization microscopy SPDM; 1997 spatially structured illumination SMI).

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Confocal microscopy

Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser confocal scanning microscopy (LCSM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation.

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Dichroic filter

A dichroic filter, thin-film filter, or interference filter is a very accurate color filter used to selectively pass light of a small range of colors while reflecting other colors.

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Fluorescence microscope

A fluorescence microscope is an optical microscope that uses fluorescence and phosphorescence instead of, or in addition to, reflection and absorption to study properties of organic or inorganic substances.

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Holography

Holography is the science and practice of making holograms.

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Leica Microsystems

Leica Microsystems GmbH is a manufacturer of optical microscopes, equipment for the preparation of microscopic specimens and related products.

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Mitochondrion

The mitochondrion (plural mitochondria) is a double-membrane-bound organelle found in most eukaryotic organisms.

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Multifocal plane microscopy

Multifocal plane microscopy (MUM) or Multiplane microscopy or Biplane microscopy is a form of light microscopy that allows the tracking of the 3D dynamics in live cells at high temporal and spatial resolution by simultaneously imaging different focal planes within the specimen.

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Objective (optics)

In optical engineering, the objective is the optical element that gathers light from the object being observed and focuses the light rays to produce a real image.

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Optical axis

An optical axis is a line along which there is some degree of rotational symmetry in an optical system such as a camera lens or microscope.

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Optical path length

In optics, optical path length (OPL) or optical distance is the product of the geometric length of the path light follows through the system, and the index of refraction of the medium through which it propagates(OP.

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Optical resolution

Optical resolution describes the ability of an imaging system to resolve detail in the object that is being imaged.

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Point spread function

The point spread function (PSF) describes the response of an imaging system to a point source or point object.

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RESOLFT

RESOLFT, an acronym for REversible Saturable Optical Linear Fluorescence Transitions, denotes a group of optical microscopy techniques with very high resolution.

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Solid angle

In geometry, a solid angle (symbol) is a measure of the amount of the field of view from some particular point that a given object covers.

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STED microscopy

Stimulated emission depletion (STED) microscopy is one of the techniques that make up super-resolution microscopy.

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Stefan Hell

Stefan Walter Hell HonFRMS (born 23 December 1962) is a Romanian-born German physicist and one of the directors of the Max Planck Institute for Biophysical Chemistry in Göttingen, Germany.

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Thomas Cremer

Thomas Cremer (born July 7, 1945 in Miesbach, Germany), is a German professor of human genetics and anthropology with a main research focus on molecular cytogenetics and 3D/4D analyses of nuclear structure studied by fluorescence microscopy including super-resolution microscopy and live cell imaging.

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Two-photon excitation microscopy

Two-photon excitation microscopy is a fluorescence imaging technique that allows imaging of living tissue up to about one millimeter in depth.

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References

[1] https://en.wikipedia.org/wiki/4Pi_microscope

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