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Colorimetry (chemical method)

Index Colorimetry (chemical method)

In physical and analytical chemistry, colorimetry or colourimetry is a technique "used to determine the concentration of colored compounds in solution." A colorimeter is a device used to test the concentration of a solution by measuring its absorbance of a specific wavelength of light (not to be confused with the tristimulus colorimeter used to measure colors in general). [1]

21 relations: Alkaline phosphatase, Analyte, Analytical chemistry, Beer–Lambert law, Bicinchoninic acid assay, Colorimeter (chemistry), Coomassie Brilliant Blue, Cuvette, Distilled water, ELISA, Jules Duboscq, Lovibond comparator, Nessler cylinder, Optical filter, Para-Nitrophenylphosphate, Permanganometry, Physical chemistry, Solution, Tristimulus colorimeter, Turbidimetry, 3,3',5,5'-Tetramethylbenzidine.

Alkaline phosphatase

Alkaline phosphatase (ALP, ALKP, ALPase, Alk Phos) or basic phosphatase is a homodimeric protein enzyme of 86 kilodaltons.

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Analyte

An analyte, component (in clinical chemistry), or chemical species is a substance or chemical constituent that is of interest in an analytical procedure.

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Analytical chemistry

Analytical chemistry studies and uses instruments and methods used to separate, identify, and quantify matter.

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Beer–Lambert law

The Beer–Lambert law, also known as Beer's law, the Lambert–Beer law, or the Beer–Lambert–Bouguer law relates the attenuation of light to the properties of the material through which the light is travelling.

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Bicinchoninic acid assay

The bicinchoninic acid assay (BCA assay), also known as the Smith assay, after its inventor, Paul K. Smith at the Pierce Chemical Company, is a biochemical assay for determining the total concentration of protein in a solution (0.5 μg/mL to 1.5 mg/mL), similar to Lowry protein assay, Bradford protein assay or biuret reagent.

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Colorimeter (chemistry)

A colorimeter is a device used in colorimetry.

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Coomassie Brilliant Blue

Coomassie Brilliant Blue is the name of two similar triphenylmethane dyes that were developed for use in the textile industry but are now commonly used for staining proteins in analytical biochemistry.

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Cuvette

A cuvette (French: cuvette.

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Distilled water

Distilled water is water that has been boiled into steam and condensed back into liquid in a separate container.

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ELISA

The enzyme-linked immunosorbent assay (ELISA) is a test that uses antibodies and color change to identify a substance.

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Jules Duboscq

Louis Jules Duboscq (March 5, 1817 – September 24, 1886) was a French instrument maker, inventor, and pioneering photographer.

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Lovibond comparator

The Lovibond comparator is an example of a colorimeter made in Britain by The Tintometer Ltd.

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Nessler cylinder

Nessler cylinders (also named color comparison cylinders or color comparing cylinders) are laboratory tubes with a fixed volume, made of glass with optically plane bottom.

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Optical filter

An optical filter is a device that selectively transmits light of different wavelengths, usually implemented as a glass plane or plastic device in the optical path, which are either dyed in the bulk or have interference coatings.

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Para-Nitrophenylphosphate

para-Nitrophenylphosphate (pNPP) is a non-proteinaceous chromogenic substrate for alkaline and acid phosphatases used in ELISA and conventional spectrophotometric assays.

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Permanganometry

Permanganometry is one of the techniques used in quantitative analysis in chemistry.

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Physical chemistry

Physical Chemistry is the study of macroscopic, atomic, subatomic, and particulate phenomena in chemical systems in terms of the principles, practices, and concepts of physics such as motion, energy, force, time, thermodynamics, quantum chemistry, statistical mechanics, analytical dynamics and chemical equilibrium.

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Solution

In chemistry, a solution is a special type of homogeneous mixture composed of two or more substances.

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Tristimulus colorimeter

A Tristimulus colorimeter, colloquially shortened to colorimeter, is used in digital imaging, to profile and calibrate output devices.

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Turbidimetry

Turbidimetry (the name being derived from turbidity) is the process of measuring the loss of intensity of transmitted light due to the scattering effect of particles suspended in it.

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3,3',5,5'-Tetramethylbenzidine

3,3’,5,5’-Tetramethylbenzidine or TMB is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA).

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Colorimetric Detector Tubes, Colorimetric detector tubes.

References

[1] https://en.wikipedia.org/wiki/Colorimetry_(chemical_method)

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