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DNA laddering

Index DNA laddering

DNA laddering is a feature that can be observed when DNA fragments, resulting from apoptotic DNA fragmentation, are visualized after separation by gel electrophoresis. [1]

19 relations: Agarose gel electrophoresis, Andrew Wyllie, Apoptosis, Apoptotic DNA fragmentation, Base pair, Caspase-activated DNase, Cell cycle analysis, DNA, Ethanol, Ethidium bromide, Flow cytometry, Gel electrophoresis, Journal of the American Society of Nephrology, Linker DNA, Morphology (biology), Nature (journal), Necrosis, TUNEL assay, University of Edinburgh Medical School.

Agarose gel electrophoresis

Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, genetics, and clinical chemistry to separate a mixed population of macromolecules such as DNA or proteins in a matrix of agarose, one of the two main components of agar.

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Andrew Wyllie

Andrew H. Wyllie FMedSci is a Scottish pathologist.

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Apoptosis

Apoptosis (from Ancient Greek ἀπόπτωσις "falling off") is a process of programmed cell death that occurs in multicellular organisms.

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Apoptotic DNA fragmentation

Apoptotic DNA fragmentation is a key feature of apoptosis, a type of programmed cell death.

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Base pair

A base pair (bp) is a unit consisting of two nucleobases bound to each other by hydrogen bonds.

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Caspase-activated DNase

Caspase-activated DNase (CAD) or DNA fragmentation factor subunit beta is a protein that in humans is encoded by the DFFB gene.

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Cell cycle analysis

Cell cycle analysis by DNA content measurement is a method that most frequently employs flow cytometry to distinguish cells in different phases of the cell cycle.

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DNA

Deoxyribonucleic acid (DNA) is a thread-like chain of nucleotides carrying the genetic instructions used in the growth, development, functioning and reproduction of all known living organisms and many viruses.

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Ethanol

Ethanol, also called alcohol, ethyl alcohol, grain alcohol, and drinking alcohol, is a chemical compound, a simple alcohol with the chemical formula.

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Ethidium bromide

Ethidium bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis.

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Flow cytometry

In biotechnology, flow cytometry is a laser- or impedance-based, biophysical technology employed in cell counting, cell sorting, biomarker detection and protein engineering, by suspending cells in a stream of fluid and passing them through an electronic detection apparatus.

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Gel electrophoresis

Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge.

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Journal of the American Society of Nephrology

The Journal of the American Society of Nephrology is a peer-reviewed medical journal covering nephrology.

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Linker DNA

Linker DNA is double-stranded DNA in between two nucleosome cores that, in association with histone H1, holds the cores together.

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Morphology (biology)

Morphology is a branch of biology dealing with the study of the form and structure of organisms and their specific structural features.

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Nature (journal)

Nature is a British multidisciplinary scientific journal, first published on 4 November 1869.

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Necrosis

Necrosis (from the Greek νέκρωσις "death, the stage of dying, the act of killing" from νεκρός "dead") is a form of cell injury which results in the premature death of cells in living tissue by autolysis.

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TUNEL assay

Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) is a method for detecting DNA fragmentation by labeling the 3′- hydroxyl termini in the double-strand DNA breaks generated during apoptosis.

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University of Edinburgh Medical School

The University of Edinburgh Medical School (also known as Edinburgh Medical School) is the medical school of the University of Edinburgh in Scotland and part of the College of Medicine and Veterinary Medicine, the head of which is Sir John Savill.

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Dna laddering.

References

[1] https://en.wikipedia.org/wiki/DNA_laddering

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