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37 relations: Arthur Kornberg, Deoxyribose, DNA, DNA clamp, DNA ligase, DNA polymerase, DNA polymerase I, DNA polymerase II, DNA polymerase IV, DNA polymerase V, DNA replication, DnaE, DnaN, DnaQ, DnaX, Enzyme, Escherichia coli, Exonuclease, Genome, HolA, HolB, HolC, HolD, HolE, Nick translation, Nucleotide, Okazaki fragments, Oregon State University, Polymerase, Primase, Primer (molecular biology), Prokaryote, Replisome, Ribose, RNA, RNA polymerase, Thomas B. Kornberg.
Arthur Kornberg
Arthur Kornberg (March 3, 1918 – October 26, 2007) was an American biochemist who won the Nobel Prize in Physiology or Medicine 1959 for his discovery of "the mechanisms in the biological synthesis of deoxyribonucleic acid (DNA)" together with Dr.
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Deoxyribose
Deoxyribose, or more precisely 2-deoxyribose, is a monosaccharide with idealized formula H−(C.
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DNA
Deoxyribonucleic acid (DNA) is a thread-like chain of nucleotides carrying the genetic instructions used in the growth, development, functioning and reproduction of all known living organisms and many viruses.
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DNA clamp
A DNA clamp, also known as a sliding clamp, is a protein fold that serves as a processivity-promoting factor in DNA replication.
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DNA ligase
DNA ligase is a specific type of enzyme, a ligase, that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond.
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DNA polymerase
DNA polymerases are enzymes that synthesize DNA molecules from deoxyribonucleotides, the building blocks of DNA.
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DNA polymerase I
DNA polymerase I (or Pol I) is an enzyme that participates in the process of prokaryotic DNA replication.
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DNA polymerase II
DNA polymerase II (also known as DNA Pol II or Pol II) is a prokaryotic DNA-Dependent DNA polymerase encoded by the PolB gene.
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DNA polymerase IV
DNA polymerase IV is a prokaryotic polymerase that is involved in mutagenesis.
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DNA polymerase V
DNA Polymerase V (Pol V) is a polymerase enzyme involved in DNA repair mechanisms in prokaryotic bacteria, such as Escherichia coli.
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DNA replication
In molecular biology, DNA replication is the biological process of producing two identical replicas of DNA from one original DNA molecule.
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DnaE
DnaE, the gene product of dnaE, is the catalytic α subunit of DNA polymerase III, acting as a DNA polymerase.
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DnaN
dnaN is the gene that codes for the DNA clamp (also known as β sliding clamp) of DNA polymerase III in prokaryotes.
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DnaQ
dnaQ is the gene encoding the ε subunit of DNA polymerase III in Escherichia coli.
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DnaX
DnaX is the gene that codes for the τ and γ subunits of the DNA clamp loader of prokaryotes.
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Enzyme
Enzymes are macromolecular biological catalysts.
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Escherichia coli
Escherichia coli (also known as E. coli) is a Gram-negative, facultatively anaerobic, rod-shaped, coliform bacterium of the genus Escherichia that is commonly found in the lower intestine of warm-blooded organisms (endotherms).
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Exonuclease
Exonucleases are enzymes that work by cleaving nucleotides one at a time from the end (exo) of a polynucleotide chain.
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Genome
In the fields of molecular biology and genetics, a genome is the genetic material of an organism.
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HolA
In molecular biology, holA is a gene that encodes the δ (delta) subunit of DNA polymerase III in E. coli and other bacteria.
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HolB
In E. coli and other bacteria, holB is a gene that encodes the delta prime subunit of DNA polymerase III.
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HolC
In E. coli and other bacteria, holC is a gene that encodes the chi subunit of DNA polymerase III.
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HolD
In E. coli and other bacteria, holD is a gene that encodes the psi subunit of DNA polymerase III.
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HolE
In E. coli and other bacteria, holE is a gene that encodes the theta subunit of DNA polymerase III.
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Nick translation
Nick translation (or head translation), developed in 1977 by Rigby and Paul Berg, is a tagging technique in molecular biology in which DNA Polymerase I is used to replace some of the nucleotides of a DNA sequence with their labeled analogues, creating a tagged DNA sequence which can be used as a probe in fluorescent in situ hybridization (FISH) or blotting techniques.
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Nucleotide
Nucleotides are organic molecules that serve as the monomer units for forming the nucleic acid polymers deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), both of which are essential biomolecules within all life-forms on Earth.
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Okazaki fragments
Okazaki fragments are short, newly synthesized DNA fragments that are formed on the lagging template strand during DNA replication.
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Oregon State University
Oregon State University (OSU) is an international, public research university in the northwest United States, located in Corvallis, Oregon.
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Polymerase
A polymerase is an enzyme (EC 2.7.7.6/7/19/48/49) that synthesizes long chains of polymers or nucleic acids.
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Primase
DNA primase is an enzyme involved in the replication of DNA and is a type of RNA polymerase.
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Primer (molecular biology)
A primer is a short strand of RNA or DNA (generally about 18-22 bases) that serves as a starting point for DNA synthesis.
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Prokaryote
A prokaryote is a unicellular organism that lacks a membrane-bound nucleus, mitochondria, or any other membrane-bound organelle.
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Replisome
The replisome is a complex molecular machine that carries out replication of DNA.
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Ribose
Ribose is a carbohydrate with the formula C5H10O5; specifically, it is a pentose monosaccharide (simple sugar) with linear form H−(C.
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RNA
Ribonucleic acid (RNA) is a polymeric molecule essential in various biological roles in coding, decoding, regulation, and expression of genes.
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RNA polymerase
RNA polymerase (ribonucleic acid polymerase), both abbreviated RNAP or RNApol, official name DNA-directed RNA polymerase, is a member of a family of enzymes that are essential to life: they are found in all organisms (-species) and many viruses.
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Thomas B. Kornberg
Thomas Bill Kornberg is an American biochemist who was the first person to purify and characterise DNA polymerase II and DNA polymerase III.
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DNA Pol III, DNA holoenzyme, DNA polymerase III, Dna polymerase iii, Pol III, PolIII, PolIII holoenzyme.
References
[1] https://en.wikipedia.org/wiki/DNA_polymerase_III_holoenzyme
