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Eastern blot

Index Eastern blot

The eastern blot is a biochemical technique used to analyze protein post translational modifications (PTM) such as lipids, phosphomoieties and glycoconjugates. [1]

52 relations: Acetylation, Acylation, Alkylation, Amino acid, Aptamer, Biotinylation, Carbohydrate, Cell membrane, Chloroplast, Concanavalin A, Current Protocols, Edwin Southern, Epitope, Eukaryote, Far-eastern blotting, Far-western blotting, Formylation, Ganglioside, Geranylgeranylation, Glycosylation, Hydroxylation, Journal of Agricultural and Food Chemistry, Lectin, Lipid, Lipoic acid, Lysosome, Messenger RNA, Methylation, Mitochondrion, Myristoylation, N-terminus, Nitrocellulose, Northern blot, Northwestern blot, Organoselenium chemistry, Palmitoylation, Phosphopantetheine, Phosphorylation, Polyvinylidene fluoride, Post-translational modification, Prenylation, Prokaryote, Protein, Red blood cell, S-Nitrosylation, SDS-PAGE, Southern blot, Succinylation, Sulfation, Transglutaminase, ..., Ubiquitin, Western blot. Expand index (2 more) »

Acetylation

Acetylation (or in IUPAC nomenclature ethanoylation) describes a reaction that introduces an acetyl functional group into a chemical compound.

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Acylation

In chemistry, acylation (rarely, but more formally: alkanoylation) is the process of adding an acyl group to a compound.

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Alkylation

Alkylation is the transfer of an alkyl group from one molecule to another.

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Amino acid

Amino acids are organic compounds containing amine (-NH2) and carboxyl (-COOH) functional groups, along with a side chain (R group) specific to each amino acid.

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Aptamer

Aptamers (from the Latin aptus – fit, and Greek meros – part) are oligonucleotide or peptide molecules that bind to a specific target molecule.

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Biotinylation

In biochemistry, biotinylation is the process of covalently attaching biotin to a protein, nucleic acid or other molecule.

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Carbohydrate

A carbohydrate is a biomolecule consisting of carbon (C), hydrogen (H) and oxygen (O) atoms, usually with a hydrogen–oxygen atom ratio of 2:1 (as in water); in other words, with the empirical formula (where m may be different from n).

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Cell membrane

The cell membrane (also known as the plasma membrane or cytoplasmic membrane, and historically referred to as the plasmalemma) is a biological membrane that separates the interior of all cells from the outside environment (the extracellular space).

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Chloroplast

Chloroplasts are organelles, specialized compartments, in plant and algal cells.

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Concanavalin A

Concanavalin A (ConA) is a lectin (carbohydrate-binding protein) originally extracted from the jack-bean, Canavalia ensiformis.

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Current Protocols

Current Protocols is a series of laboratory manuals for life scientists.

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Edwin Southern

Sir Edwin Mellor Southern, FRS FRSE (born 7 June 1938) is an English Lasker Award-winning molecular biologist, Emeritus Professor of Biochemistry at the University of Oxford and a fellow of Trinity College, Oxford.

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Epitope

An epitope, also known as antigenic determinant, is the part of an antigen that is recognized by the immune system, specifically by antibodies, B cells, or T cells.

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Eukaryote

Eukaryotes are organisms whose cells have a nucleus enclosed within membranes, unlike Prokaryotes (Bacteria and other Archaea).

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Far-eastern blotting

Far-eastern blotting is a technique for the analysis of lipids separated by high-performance thin layer chromatography (HPTLC).

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Far-western blotting

Far-western blotting is a molecular biological method which is based on the technique of western blotting to detect protein-protein interaction in vitro.

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Formylation

In biochemistry, the addition of a formyl functional group is termed formylation.

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Ganglioside

A ganglioside is a molecule composed of a glycosphingolipid (ceramide and oligosaccharide) with one or more sialic acids (e.g. n-acetylneuraminic acid, NANA) linked on the sugar chain.

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Geranylgeranylation

Geranylgeranylation is a form of prenylation, which is a post-translational modification of proteins that involves the attachment of one or two 20-carbon lipophilic geranylgeranyl isoprene units from geranylgeranyl diphosphate to one or two cysteine residue(s) at the C-terminus of specific proteins.

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Glycosylation

Glycosylation (see also chemical glycosylation) is the reaction in which a carbohydrate, i.e. a glycosyl donor, is attached to a hydroxyl or other functional group of another molecule (a glycosyl acceptor).

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Hydroxylation

Hydroxylation is a chemical process that introduces a hydroxyl group (-OH) into an organic compound.

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Journal of Agricultural and Food Chemistry

The Journal of Agricultural and Food Chemistry is a weekly peer-reviewed scientific journal established in 1953 by the American Chemical Society.

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Lectin

Lectins are carbohydrate-binding proteins, macromolecules that are highly specific for sugar moieties of other molecules.

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Lipid

In biology and biochemistry, a lipid is a biomolecule that is soluble in nonpolar solvents.

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Lipoic acid

Lipoic acid (LA), also known as α-lipoic acid and alpha lipoic acid (ALA) and thioctic acid is an organosulfur compound derived from caprylic acid (octanoic acid).

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Lysosome

A lysosome is a membrane-bound organelle found in nearly all animal cells.

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Messenger RNA

Messenger RNA (mRNA) is a large family of RNA molecules that convey genetic information from DNA to the ribosome, where they specify the amino acid sequence of the protein products of gene expression.

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Methylation

In the chemical sciences, methylation denotes the addition of a methyl group on a substrate, or the substitution of an atom (or group) by a methyl group.

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Mitochondrion

The mitochondrion (plural mitochondria) is a double-membrane-bound organelle found in most eukaryotic organisms.

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Myristoylation

Myristoylation is a lipidation modification where a myristoyl group, derived from myristic acid, is covalently attached by an amide bond to the alpha-amino group of an N-terminal glycine residue.

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N-terminus

The N-terminus (also known as the amino-terminus, NH2-terminus, N-terminal end or amine-terminus) is the start of a protein or polypeptide referring to the free amine group (-NH2) located at the end of a polypeptide.

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Nitrocellulose

Nitrocellulose (also known as cellulose nitrate, flash paper, flash cotton, guncotton, and flash string) is a highly flammable compound formed by nitrating cellulose through exposure to nitric acid or another powerful nitrating agent.

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Northern blot

The northern blot, or RNA blot,Gilbert, S. F. (2000) Developmental Biology, 6th Ed.

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Northwestern blot

The Northwestern blot, also known as the Northwestern assay, is a hybrid analytical technique of the Western blot and the Northern blot, and is used in molecular biology to detect interactions between RNA and proteins.

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Organoselenium chemistry

Organoselenium compounds are chemical compounds containing carbon-to-selenium chemical bonds.

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Palmitoylation

Palmitoylation is the covalent attachment of fatty acids, such as palmitic acid, to cysteine and less frequently to serine and threonine residues of proteins, which are typically membrane proteins.

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Phosphopantetheine

Phosphopantetheine, also known as 4'-Phosphopantetheine, is an essential prosthetic group of acyl carrier protein (ACP) and peptidyl carrier proteins (PCP) and aryl carrier proteins (ArCP) derived from Coenzyme A. It is also present in formyltetrahydrofolate dehydrogenase.

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Phosphorylation

In chemistry, phosphorylation of a molecule is the attachment of a phosphoryl group.

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Polyvinylidene fluoride

Polyvinylidene fluoride or polyvinylidene difluoride (PVDF) is a highly non-reactive thermoplastic fluoropolymer produced by the polymerization of vinylidene difluoride.

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Post-translational modification

Post-translational modification (PTM) refers to the covalent and generally enzymatic modification of proteins following protein biosynthesis.

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Prenylation

Prenylation (also known as isoprenylation or lipidation) is the addition of hydrophobic molecules to a protein or chemical compound.

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Prokaryote

A prokaryote is a unicellular organism that lacks a membrane-bound nucleus, mitochondria, or any other membrane-bound organelle.

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Protein

Proteins are large biomolecules, or macromolecules, consisting of one or more long chains of amino acid residues.

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Red blood cell

Red blood cells-- also known as RBCs, red cells, red blood corpuscles, haematids, erythroid cells or erythrocytes (from Greek erythros for "red" and kytos for "hollow vessel", with -cyte translated as "cell" in modern usage), are the most common type of blood cell and the vertebrate's principal means of delivering oxygen (O2) to the body tissues—via blood flow through the circulatory system.

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S-Nitrosylation

S-Nitrosylation, the covalent attachment of NO to a cysteine residue to form an ''S''-nitrosothiol (SNO), is a post-translational protein modification of broad purview across phylogeny and cell types.

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SDS-PAGE

SDS-PAGE (sodium dodecyl sulfate–polyacrylamide gel electrophoresis) is a variant of polyacrylamide gel electrophoresis, an analytical method in biochemistry for the separation of charged molecules in mixtures by their molecular masses in an electric field.

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Southern blot

A Southern blot is a method used in molecular biology for detection of a specific DNA sequence in DNA samples.

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Succinylation

Succinylation is a posttranslational modification where a succinyl group (-CO-CH2-CH2-CO2H) is added to a lysine residue of a protein molecule.

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Sulfation

Sulfation or sulfurylation (not to be confused with sulfonation) in biochemistry is the enzyme-catalyzed conjugation of a sulfo group (not a sulfate or sulfuryl group) to another molecule.

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Transglutaminase

A transglutaminase is an enzyme that catalyzes the formation of an isopeptide bond between a free amine group (e.g., protein- or peptide-bound lysine) and the acyl group at the end of the side chain of protein- or peptide-bound glutamine.

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Ubiquitin

Ubiquitin is a small (8.5 kDa) regulatory protein found in most tissues of eukaryotic organisms, i.e. it occurs ''ubiquitously''.

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Western blot

The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used in molecular biology, immunogenetics and other molecular biology disciplines to detect specific proteins in a sample of tissue homogenate or extract.

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References

[1] https://en.wikipedia.org/wiki/Eastern_blot

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