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Fluorescence spectroscopy

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Fluorescence spectroscopy (also known as fluorometry or spectrofluorometry) is a type of electromagnetic spectroscopy that analyzes fluorescence from a sample. [1]

42 relations: Absorption spectroscopy, Amphiphile, Chemometrics, Cold vapour atomic fluorescence spectroscopy, Collimated light, Concentration, Diffraction grating, Energy level, Filter fluorometer, Fluorescence, Fluorometer, Fluorophore, High-performance liquid chromatography, Intensity (physics), Jablonski diagram, Lanthanide probes, Light, Mercury-vapor lamp, Micelle, Microfluorimetry, Molecule, Monochromator, Organic compound, Phenylalanine, Photoluminescence, Photon, Protein folding, Quenching (fluorescence), Raman scattering, Rayleigh scattering, Solvatochromism, Spectrofluorometer, Spectroscopy, Stationary state, Stray light, Surfactant, Tryptophan, Tyrosine, Ultraviolet, Vesicle (biology and chemistry), Virtual state, Xenon arc lamp.

Absorption spectroscopy

Absorption spectroscopy refers to spectroscopic techniques that measure the absorption of radiation, as a function of frequency or wavelength, due to its interaction with a sample.

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An amphiphile (from the Greek αμφις, amphis: both and φιλíα, philia: love, friendship) is a chemical compound possessing both hydrophilic (water-loving, polar) and lipophilic (fat-loving) properties.

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Chemometrics is the science of extracting information from chemical systems by data-driven means.

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Cold vapour atomic fluorescence spectroscopy

Cold vapour atomic fluorescence spectroscopy (CVAFS) is a subset of the analytical technique known as atomic fluorescence spectroscopy (AFS).

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Collimated light

Collimated light is light whose rays are parallel, and therefore will spread minimally as it propagates.

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In chemistry, concentration is the abundance of a constituent divided by the total volume of a mixture.

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Diffraction grating

In optics, a diffraction grating is an optical component with a periodic structure that splits and diffracts light into several beams travelling in different directions.

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Energy level

A quantum mechanical system or particle that is bound—that is, confined spatially—can only take on certain discrete values of energy.

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Filter fluorometer

A filter fluorometer is a type of fluorometer that may be employed in fluorescence spectroscopy.

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Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation.

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A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light.

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A fluorophore (or fluorochrome, similarly to a chromophore) is a fluorescent chemical compound that can re-emit light upon light excitation.

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High-performance liquid chromatography

High-performance liquid chromatography (HPLC; formerly referred to as high-pressure liquid chromatography), is a technique in analytical chemistry used to separate, identify, and quantify each component in a mixture.

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Intensity (physics)

In physics, intensity is the power transferred per unit area, where the area is measured on the plane perpendicular to the direction of propagation of the energy.

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Jablonski diagram

In molecular spectroscopy, a Jablonski diagram is a diagram that illustrates the electronic states of a molecule and the transitions between them.

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Lanthanide probes

Lanthanide probes are a non-invasive analytical tool commonly used for biological and chemical applications.

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Light is electromagnetic radiation within a certain portion of the electromagnetic spectrum.

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Mercury-vapor lamp

A mercury-vapor lamp is a gas discharge lamp that uses an electric arc through vaporized mercury to produce light.

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A micelle or micella (plural micelles or micellae, respectively) is an aggregate (or supramolecular assembly) of surfactant molecules dispersed in a liquid colloid.

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Microfluorimetry is an adaption of fluorimetry for studying the biochemical and biophysical properties of cells by using microscopy to image cell components tagged with fluorescent molecules.

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A molecule is an electrically neutral group of two or more atoms held together by chemical bonds.

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A monochromator is an optical device that transmits a mechanically selectable narrow band of wavelengths of light or other radiation chosen from a wider range of wavelengths available at the input.

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Organic compound

In chemistry, an organic compound is generally any chemical compound that contains carbon.

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Phenylalanine (symbol Phe or F) is an α-amino acid with the formula.

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Photoluminescence (abbreviated as PL) is light emission from any form of matter after the absorption of photons (electromagnetic radiation).

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The photon is a type of elementary particle, the quantum of the electromagnetic field including electromagnetic radiation such as light, and the force carrier for the electromagnetic force (even when static via virtual particles).

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Protein folding

Protein folding is the physical process by which a protein chain acquires its native 3-dimensional structure, a conformation that is usually biologically functional, in an expeditious and reproducible manner.

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Quenching (fluorescence)

Quenching refers to any process which decreases the fluorescence intensity of a given substance.

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Raman scattering

Raman scattering or the Raman effect is the inelastic scattering of a photon by molecules which are excited to higher vibrational or rotational energy levels.

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Rayleigh scattering

Rayleigh scattering (pronounced), named after the British physicist Lord Rayleigh (John William Strutt), is the (dominantly) elastic scattering of light or other electromagnetic radiation by particles much smaller than the wavelength of the radiation.

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Solvatochromism is the ability of a chemical substance to change color due to a change in solvent polarity.

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A spectrofluorometer is an instrument which takes advantage of fluorescent properties of some compounds in order to provide information regarding their concentration and chemical environment in a sample.

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Spectroscopy is the study of the interaction between matter and electromagnetic radiation.

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Stationary state

A stationary state is a quantum state with all observables independent of time.

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Stray light

Stray light is light in an optical system, which was not intended in the design.

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Surfactants are compounds that lower the surface tension (or interfacial tension) between two liquids, between a gas and a liquid, or between a liquid and a solid.

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Tryptophan (symbol Trp or W) is an α-amino acid that is used in the biosynthesis of proteins.

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Tyrosine (symbol Tyr or Y) or 4-hydroxyphenylalanine is one of the 20 standard amino acids that are used by cells to synthesize proteins.

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Ultraviolet (UV) is electromagnetic radiation with a wavelength from 10 nm to 400 nm, shorter than that of visible light but longer than X-rays.

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Vesicle (biology and chemistry)

In cell biology, a vesicle is a small structure within a cell, or extracellular, consisting of fluid enclosed by a lipid bilayer.

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Virtual state

In quantum physics, a virtual state is a very short-lived, unobservable quantum state.

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Xenon arc lamp

A xenon arc lamp is a highly specialized type of gas discharge lamp, an electric light that produces light by passing electricity through ionized xenon gas at high pressure.

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Atomic fluorescence spectroscopy, Emission spectrum (fluorescence spectroscopy), Excitation spectrum, Fluorescence Spectroscopy, Fluorescence spectrum, Fluorimetry, Fluorometric, Fluorometric analysis, Fluorometry, Spectrofluorimetry, Time-resolved fluorescence spectroscopy, Time-resolved fluoresence spectroscopy, Tryptophan fluorescence spectroscopy.


[1] https://en.wikipedia.org/wiki/Fluorescence_spectroscopy

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