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HNCA experiment

Index HNCA experiment

HNCA is a 3D triple-resonance NMR experiment commonly used in the field of protein NMR. [1]

9 relations: Amino acid, Carbon-13, HNCOCA experiment, Isotopes of nitrogen, Isotopic labeling, Nuclear magnetic resonance, Nuclear magnetic resonance spectroscopy of proteins, Recombinant DNA, Triple-resonance nuclear magnetic resonance spectroscopy.

Amino acid

Amino acids are organic compounds containing amine (-NH2) and carboxyl (-COOH) functional groups, along with a side chain (R group) specific to each amino acid.

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Carbon-13

Carbon-13 (13C) is a natural, stable isotope of carbon with a nucleus containing six protons and seven neutrons.

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HNCOCA experiment

HNCOCA is a 3D triple-resonance NMR experiment commonly used in the field of protein NMR.

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Isotopes of nitrogen

Natural nitrogen (7N) consists of two stable isotopes, nitrogen-14, which makes up the vast majority of naturally occurring nitrogen, and nitrogen-15, which is less common.

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Isotopic labeling

Isotopic labeling (or isotopic labelling) is a technique used to track the passage of an isotope (an atom with a detectable variation) through a reaction, metabolic pathway, or cell.

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Nuclear magnetic resonance

Nuclear magnetic resonance (NMR) is a physical phenomenon in which nuclei in a magnetic field absorb and re-emit electromagnetic radiation.

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Nuclear magnetic resonance spectroscopy of proteins

Nuclear magnetic resonance spectroscopy of proteins (usually abbreviated protein NMR) is a field of structural biology in which NMR spectroscopy is used to obtain information about the structure and dynamics of proteins, and also nucleic acids, and their complexes.

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Recombinant DNA

Recombinant DNA (rDNA) molecules are DNA molecules formed by laboratory methods of genetic recombination (such as molecular cloning) to bring together genetic material from multiple sources, creating sequences that would not otherwise be found in the genome.

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Triple-resonance nuclear magnetic resonance spectroscopy

Triple resonance experiments are a set of multi-dimensional nuclear magnetic resonance spectroscopy (NMR) experiments that link three types of atomic nuclei, most typically consisting of 1H, 15N and 13C.

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References

[1] https://en.wikipedia.org/wiki/HNCA_experiment

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