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Immunofluorescence

Index Immunofluorescence

Immunofluorescence is a technique used for light microscopy with a fluorescence microscope and is used primarily on microbiological samples. [1]

33 relations: Alexa Fluor, Antibody, Antigen, Basement membrane, Biomolecule, Confocal microscopy, DAPI, Davidson College, Dermis, DNA, Dye, DyLight Fluor, Epidermis, Fluorescence, Fluorescence microscope, Fluorophore, Glycan, Green fluorescent protein, Henoch–Schönlein purpura, Immortalised cell line, Immunochemistry, Immunohistochemistry, Immunostaining, List of immunofluorescence findings for autoimmune bullous conditions, Lupus erythematosus, Microbiology, Microscopy, Optical microscope, Patching and Capping, Photobleaching, Protein, Recombinant DNA, University of Birmingham.

Alexa Fluor

The Alexa Fluor family of fluorescent dyes is a series of dyes invented by Molecular Probes, now a part of Thermo Fisher Scientific, and sold under the Invitrogen brand name.

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Antibody

An antibody (Ab), also known as an immunoglobulin (Ig), is a large, Y-shaped protein produced mainly by plasma cells that is used by the immune system to neutralize pathogens such as pathogenic bacteria and viruses.

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Antigen

In immunology, an antigen is a molecule capable of inducing an immune response (to produce an antibody) in the host organism.

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Basement membrane

The basement membrane is a thin, fibrous, extracellular matrix of tissue that separates the lining of an internal or external body surface from underlying connective tissue in metazoans.

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Biomolecule

A biomolecule or biological molecule is a loosely used term for molecules and ions that are present in organisms, essential to some typically biological process such as cell division, morphogenesis, or development.

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Confocal microscopy

Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser confocal scanning microscopy (LCSM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation.

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DAPI

DAPI, or 4′,6-diamidino-2-phenylindole, is a fluorescent stain that binds strongly to adenine–thymine rich regions in DNA.

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Davidson College

Davidson College is a private liberal arts college in Davidson, North Carolina with a historic 665-acre main campus and a 110-acre lake campus on Lake Norman.

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Dermis

The dermis or corium is a layer of skin between the epidermis (with which it makes up the cutis) and subcutaneous tissues, that primarily consists of dense irregular connective tissue and cushions the body from stress and strain.

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DNA

Deoxyribonucleic acid (DNA) is a thread-like chain of nucleotides carrying the genetic instructions used in the growth, development, functioning and reproduction of all known living organisms and many viruses.

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Dye

A dye is a colored substance that has an affinity to the substrate to which it is being applied.

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DyLight Fluor

The DyLight Fluor family of fluorescent dyes are produced by Dyomics in collaboration with Thermo Fisher Scientific.

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Epidermis

The epidermis is the outer layer of the three layers that make up the skin, the inner layers being the dermis and hypodermis.

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Fluorescence

Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation.

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Fluorescence microscope

A fluorescence microscope is an optical microscope that uses fluorescence and phosphorescence instead of, or in addition to, reflection and absorption to study properties of organic or inorganic substances.

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Fluorophore

A fluorophore (or fluorochrome, similarly to a chromophore) is a fluorescent chemical compound that can re-emit light upon light excitation.

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Glycan

The terms glycan and polysaccharide are defined by IUPAC as synonyms meaning "compounds consisting of a large number of monosaccharides linked glycosidically".

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Green fluorescent protein

The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range.

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Henoch–Schönlein purpura

Henoch–Schönlein purpura (HSP) also known as IgA vasculitis, anaphylactoid purpura, purpura rheumatica, and Schönlein–Henoch purpura, is a disease of the skin, mucous membranes, and sometimes other organs that most commonly affects children.

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Immortalised cell line

An immortalized cell line is a population of cells from a multicellular organism which would normally not proliferate indefinitely but, due to mutation, have evaded normal cellular senescence and instead can keep undergoing division.

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Immunochemistry

Immunochemistry is a branch of chemistry that involves the study of the molecular mechanisms underlying the function of the immune system, especially the nature of antibodies, antigens and their interactions.

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Immunohistochemistry

Immunohistochemistry (IHC) involves the process of selectively imaging antigens (proteins) in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues.

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Immunostaining

In biochemistry, immunostaining is any use of an antibody-based method to detect a specific protein in a sample.

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List of immunofluorescence findings for autoimmune bullous conditions

Several cutaneous conditions can be diagnosed with the aid of immunofluorescence studies.

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Lupus erythematosus

Lupus erythematosus is a collection of autoimmune diseases in which the human immune system becomes hyperactive and attacks healthy tissues.

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Microbiology

Microbiology (from Greek μῑκρος, mīkros, "small"; βίος, bios, "life"; and -λογία, -logia) is the study of microorganisms, those being unicellular (single cell), multicellular (cell colony), or acellular (lacking cells).

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Microscopy

Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye (objects that are not within the resolution range of the normal eye).

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Optical microscope

The optical microscope, often referred to as the light microscope, is a type of microscope that uses visible light and a system of lenses to magnify images of small subjects.

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Patching and Capping

The aggregation of fluorescently tagged antibodies that are associated with proteins on membranes of living cells.

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Photobleaching

In optics, photobleaching (sometimes termed fading) is the photochemical alteration of a dye or a fluorophore molecule such that it permanently is unable to fluoresce.

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Protein

Proteins are large biomolecules, or macromolecules, consisting of one or more long chains of amino acid residues.

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Recombinant DNA

Recombinant DNA (rDNA) molecules are DNA molecules formed by laboratory methods of genetic recombination (such as molecular cloning) to bring together genetic material from multiple sources, creating sequences that would not otherwise be found in the genome.

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University of Birmingham

The University of Birmingham (informally Birmingham University) is a public research university located in Edgbaston, Birmingham, United Kingdom.

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Redirects here:

Fluorescent antibody technique, Immunoflourescence, Immunoflourescence assay, Immunofluorescence assay, Immunofluorescence microscopy, Immunofluorescent, Immunostaining protocol, Indirect Fluorescent Antibody, Indirect fluorescent antibody, Indirect fluorescent antibody technique, Indirect fluorescent-antibody, Indirect immunofluorescence, Life Science Protocols, Micro-immunofluorescence, Microimmunofluorescence.

References

[1] https://en.wikipedia.org/wiki/Immunofluorescence

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