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In-gel digestion

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The in-gel digestion is part of the sample preparation for the mass spectrometric identification of proteins in course of proteomic analysis. [1]

83 relations: Acetonitrile, Acrylamide, Adsorption, Alkylation, Amino acid, Ammonium bicarbonate, Arginine, Aspartic acid, Automation, Biomolecular structure, Buffer solution, C-terminus, Calcium, Centrifugal evaporator, Cleavable detergent, Contamination, Coomassie Brilliant Blue, Coordination complex, Cysteine, Cystine, Dehydration, Disulfide, Dithiothreitol, Dye, Electric charge, Electrophoresis, Electrospray ionization, Electrostatics, Enzyme, Extraction (chemistry), Formic acid, Glutamic acid, Hydrogen peroxide, Hydrolysis, Iodoacetamide, Ion, Ionization, Keratin, Lipophilicity, List of Mega Man characters, Lys-N, Lysine, Mass spectrometry, Matrix-assisted laser desorption/ionization, Membrane protein, Merck Millipore, Methylation, Monomer, Organic compound, Peptide, ..., Peptide bond, PH, Phosphine, Pipette, Polyacrylamide gel electrophoresis, Potassium ferricyanide, Proline, Protease, Protein, Proteomics, Quantity, Redox, Robot, Salt, Sample preparation in mass spectrometry, Sequence, Serine protease, Silver, Silver stain, Sodium thiosulfate, Solvent, Standardization, Surfactant, TCEP, Temperature, Thiol, Trifluoroacetic acid, Trypsin, Unified atomic mass unit, Vacuum pump, Volatility (chemistry), Water, Zymography. Expand index (33 more) »


Acetonitrile is the chemical compound with the formula.

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Acrylamide (or acrylic amide) is a chemical compound with the chemical formula C3H5NO.

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Adsorption is the adhesion of atoms, ions or molecules from a gas, liquid or dissolved solid to a surface.

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Alkylation is the transfer of an alkyl group from one molecule to another.

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Amino acid

Amino acids are organic compounds containing amine (-NH2) and carboxyl (-COOH) functional groups, along with a side chain (R group) specific to each amino acid.

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Ammonium bicarbonate

Ammonium bicarbonate is an inorganic compound with formula (NH4)HCO3, simplified to NH5CO3.

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Arginine (symbol Arg or R) is an α-amino acid that is used in the biosynthesis of proteins.

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Aspartic acid

Aspartic acid (symbol Asp or D; salts known as aspartates), is an α-amino acid that is used in the biosynthesis of proteins.

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Automation is the technology by which a process or procedure is performed without human assistance.

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Biomolecular structure

Biomolecular structure is the intricate folded, three-dimensional shape that is formed by a molecule of protein, DNA, or RNA, and that is important to its function.

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Buffer solution

A buffer solution (more precisely, pH buffer or hydrogen ion buffer) is an aqueous solution consisting of a mixture of a weak acid and its conjugate base, or vice versa.

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The C-terminus (also known as the carboxyl-terminus, carboxy-terminus, C-terminal tail, C-terminal end, or COOH-terminus) is the end of an amino acid chain (protein or polypeptide), terminated by a free carboxyl group (-COOH).

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Calcium is a chemical element with symbol Ca and atomic number 20.

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Centrifugal evaporator

A centrifugal evaporator is a device used in chemical and biochemical laboratories for the efficient and gentle evaporation of solvents from many samples at the same time, and samples contained in microtitre plates.

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Cleavable detergent

Cleavable detergents, also known as cleavable surfactants, are special surfactants (detergents) that are used in biochemistry and especially in proteomics to enhance protein denaturation and solubility.

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Contamination is the presence of an unwanted constituent, contaminant or impurity in a material, physical body, natural environment, workplace, etc.

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Coomassie Brilliant Blue

Coomassie Brilliant Blue is the name of two similar triphenylmethane dyes that were developed for use in the textile industry but are now commonly used for staining proteins in analytical biochemistry.

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Coordination complex

In chemistry, a coordination complex consists of a central atom or ion, which is usually metallic and is called the coordination centre, and a surrounding array of bound molecules or ions, that are in turn known as ligands or complexing agents.

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Cysteine (symbol Cys or C) is a semi-essential proteinogenic amino acid with the formula HO2CCH(NH2)CH2SH.

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Cystine is the oxidized dimer form of the amino acid cysteine and has the formula (SCH2CH(NH2)CO2H)2.

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In physiology, dehydration is a deficit of total body water, with an accompanying disruption of metabolic processes.

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In chemistry, a disulfide refers to a functional group with the structure R−S−S−R′.

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Dithiothreitol (DTT) is the common name for a small-molecule redox reagent also known as Cleland's reagent.

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A dye is a colored substance that has an affinity to the substrate to which it is being applied.

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Electric charge

Electric charge is the physical property of matter that causes it to experience a force when placed in an electromagnetic field.

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Electrophoresis (from the Greek "Ηλεκτροφόρηση" meaning "to bear electrons") is the motion of dispersed particles relative to a fluid under the influence of a spatially uniform electric field.

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Electrospray ionization

Electrospray ionization (ESI) is a technique used in mass spectrometry to produce ions using an electrospray in which a high voltage is applied to a liquid to create an aerosol.

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Electrostatics is a branch of physics that studies electric charges at rest.

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Enzymes are macromolecular biological catalysts.

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Extraction (chemistry)

Extraction in chemistry is a separation process consisting in the separation of a substance from a matrix.

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Formic acid

Formic acid, systematically named methanoic acid, is the simplest carboxylic acid.

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Glutamic acid

Glutamic acid (symbol Glu or E) is an α-amino acid with formula.

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Hydrogen peroxide

Hydrogen peroxide is a chemical compound with the formula.

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Hydrolysis is a term used for both an electro-chemical process and a biological one.

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2-Iodoacetamide is an alkylating agent used for peptide mapping purposes.

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An ion is an atom or molecule that has a non-zero net electrical charge (its total number of electrons is not equal to its total number of protons).

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Ionization or ionisation, is the process by which an atom or a molecule acquires a negative or positive charge by gaining or losing electrons to form ions, often in conjunction with other chemical changes.

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Keratin is one of a family of fibrous structural proteins.

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Lipophilicity (from Greek λίπος "fat" and φίλος "friendly"), refers to the ability of a chemical compound to dissolve in fats, oils, lipids, and non-polar solvents such as hexane or toluene.

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List of Mega Man characters

This is a list of characters from the Mega Man series.

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Lys-N is a metalloendopeptidase found in the mushroom Grifola frondosa that cleaves proteins on the amino side of lysine residues.

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Lysine (symbol Lys or K) is an α-amino acid that is used in the biosynthesis of proteins.

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Mass spectrometry

Mass spectrometry (MS) is an analytical technique that ionizes chemical species and sorts the ions based on their mass-to-charge ratio.

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Matrix-assisted laser desorption/ionization

In mass spectrometry, matrix-assisted laser desorption/ionization (MALDI) is an ionization technique that uses a laser energy absorbing matrix to create ions from large molecules with minimal fragmentation.

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Membrane protein

Membrane proteins are proteins that interact with, or are part of, biological membranes.

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Merck Millipore

Merck Millipore was the brand used for Merck's global life science business until 2015 when the company re-branded.

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In the chemical sciences, methylation denotes the addition of a methyl group on a substrate, or the substitution of an atom (or group) by a methyl group.

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A monomer (mono-, "one" + -mer, "part") is a molecule that "can undergo polymerization thereby contributing constitutional units to the essential structure of a macromolecule".

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Organic compound

In chemistry, an organic compound is generally any chemical compound that contains carbon.

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Peptides (from Gr.: πεπτός, peptós "digested"; derived from πέσσειν, péssein "to digest") are short chains of amino acid monomers linked by peptide (amide) bonds.

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Peptide bond

A peptide bond is a covalent chemical bond linking two consecutive amino acid monomers along a peptide or protein chain.

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In chemistry, pH is a logarithmic scale used to specify the acidity or basicity of an aqueous solution.

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Phosphine (IUPAC name: phosphane) is the compound with the chemical formula PH3.

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A pipette (sometimes spelled pipet) is a laboratory tool commonly used in chemistry, biology and medicine to transport a measured volume of liquid, often as a media dispenser.

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Polyacrylamide gel electrophoresis

Polyacrylamide gel electrophoresis (PAGE) is a technique widely used in biochemistry, forensic chemistry, genetics, molecular biology and biotechnology to separate biological macromolecules, usually proteins or nucleic acids, according to their electrophoretic mobility.

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Potassium ferricyanide

Potassium ferricyanide is the chemical compound with the formula K3.

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Proline (symbol Pro or P) is a proteinogenic amino acid that is used in the biosynthesis of proteins.

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A protease (also called a peptidase or proteinase) is an enzyme that performs proteolysis: protein catabolism by hydrolysis of peptide bonds.

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Proteins are large biomolecules, or macromolecules, consisting of one or more long chains of amino acid residues.

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Proteomics is the large-scale study of proteins.

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Quantity is a property that can exist as a multitude or magnitude.

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Redox (short for reduction–oxidation reaction) (pronunciation: or) is a chemical reaction in which the oxidation states of atoms are changed.

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A robot is a machine—especially one programmable by a computer— capable of carrying out a complex series of actions automatically.

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Salt, table salt or common salt is a mineral composed primarily of sodium chloride (NaCl), a chemical compound belonging to the larger class of salts; salt in its natural form as a crystalline mineral is known as rock salt or halite.

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Sample preparation in mass spectrometry

Sample preparation for mass spectrometry is used for the optimization of a sample for analysis in a mass spectrometer (MS).

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In mathematics, a sequence is an enumerated collection of objects in which repetitions are allowed.

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Serine protease

Serine proteases (or serine endopeptidases) are enzymes that cleave peptide bonds in proteins, in which serine serves as the nucleophilic amino acid at the (enzyme's) active site.

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Silver is a chemical element with symbol Ag (from the Latin argentum, derived from the Proto-Indo-European ''h₂erǵ'': "shiny" or "white") and atomic number 47.

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Silver stain

Silver staining is the use of silver to selectively alter the appearance of a target in microscopy of histological sections; in temperature gradient gel electrophoresis; and in polyacrylamide gels.

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Sodium thiosulfate

Sodium thiosulfate (sodium thiosulphate) is a chemical and medication.

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A solvent (from the Latin solvō, "loosen, untie, solve") is a substance that dissolves a solute (a chemically distinct liquid, solid or gas), resulting in a solution.

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Standardization or standardisation is the process of implementing and developing technical standards based on the consensus of different parties that include firms, users, interest groups, standards organizations and governments Standardization can help to maximize compatibility, interoperability, safety, repeatability, or quality.

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Surfactants are compounds that lower the surface tension (or interfacial tension) between two liquids, between a gas and a liquid, or between a liquid and a solid.

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TCEP (tris(2-carboxyethyl)phosphine) is a reducing agent frequently used in biochemistry and molecular biology applications.

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Temperature is a physical quantity expressing hot and cold.

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Thiol is an organosulfur compound that contains a carbon-bonded sulfhydryl (R–SH) group (where R represents an alkyl or other organic substituent).

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Trifluoroacetic acid

Trifluoroacetic acid (TFA) is an organofluorine compound with the chemical formula CF3CO2H.

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Trypsin is a serine protease from the PA clan superfamily, found in the digestive system of many vertebrates, where it hydrolyzes proteins.

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Unified atomic mass unit

The unified atomic mass unit or dalton (symbol: u, or Da) is a standard unit of mass that quantifies mass on an atomic or molecular scale (atomic mass).

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Vacuum pump

A vacuum pump is a device that removes gas molecules from a sealed volume in order to leave behind a partial vacuum.

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Volatility (chemistry)

In chemistry and physics, volatility is quantified by the tendency of a substance to vaporize.

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Water is a transparent, tasteless, odorless, and nearly colorless chemical substance that is the main constituent of Earth's streams, lakes, and oceans, and the fluids of most living organisms.

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Zymography is an electrophoretic technique for the detection of hydrolytic enzymes, based on the substrate repertoire of the enzyme.

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[1] https://en.wikipedia.org/wiki/In-gel_digestion

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