MS2 tagging

MS2 tagging is a technique based upon the natural interaction of the MS2 bacteriophage coat protein with a stem-loop structure from the phage genome, which is used for biochemical purification of RNA-protein complexes and partnered to GFP for detection of RNA in living cells. ^[1]

8 relations: Bacteriophage MS2, Capsid, Green fluorescent protein, Nuclear localization sequence, Oligomer, Ribonucleoprotein particle, Stem-loop, Transcriptional bursting.

Bacteriophage MS2

The bacteriophage MS2 is an icosahedral, positive-sense single-stranded RNA virus that infects the bacterium Escherichia coli and other members of the Enterobacteriaceae.

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Capsid

A capsid is the protein shell of a virus.

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Green fluorescent protein

The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range.

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Nuclear localization sequence

A nuclear localization signal or sequence (NLS) is an amino acid sequence that 'tags' a protein for import into the cell nucleus by nuclear transport.

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Oligomer

An oligomer (oligo-, "a few" + -mer, "parts") is a molecular complex of chemicals that consists of a few monomer units, in contrast to a polymer, where the number of monomers is, in principle, infinite.

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Ribonucleoprotein particle

A ribonucleoprotein particle or RNP is a complex formed between RNA and RNA-binding proteins (RBPs).

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Stem-loop

Stem-loop intramolecular base pairing is a pattern that can occur in single-stranded DNA or, more commonly, in RNA.

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Transcriptional bursting

Transcriptional bursting, also known as transcriptional pulsing, is a fundamental property of genes in which transcription from DNA to RNA can occur in "bursts" or "pulses", which has been observed in diverse organisms, from bacteria to mammals.

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References

[1] https://en.wikipedia.org/wiki/MS2_tagging

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