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Nick (DNA)

Index Nick (DNA)

A nick is a discontinuity in a double stranded DNA molecule where there is no phosphodiester bond between adjacent nucleotides of one strand typically through damage or enzyme action. [1]

29 relations: Active site, Adenosine monophosphate, Deamination, DNA, DNA ligase, DNA methylation, DNA mismatch repair, DNA polymerase, DNA replication, Enzyme, Escherichia coli, Eukaryote, Genetic recombination, Immunofluorescence, In vitro, Ligase, Ligation (molecular biology), Nick translation, Nicking enzyme, Nicotinamide adenine dinucleotide, Nucleic acid double helix, Nucleic acid structure, Nucleotide, Okazaki fragments, Phosphodiester bond, Ribonucleotide, RNASEH2A, Type I topoisomerase, Ultraviolet.

Active site

In biology, the active site is the region of an enzyme where substrate molecules bind and undergo a chemical reaction.

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Adenosine monophosphate

Adenosine monophosphate (AMP), also known as 5'-adenylic acid, is a nucleotide.

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Deamination

Deamination is the removal of an amine group from a protein molecule.

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DNA

Deoxyribonucleic acid (DNA) is a thread-like chain of nucleotides carrying the genetic instructions used in the growth, development, functioning and reproduction of all known living organisms and many viruses.

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DNA ligase

DNA ligase is a specific type of enzyme, a ligase, that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond.

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DNA methylation

DNA methylation is a process by which methyl groups are added to the DNA molecule.

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DNA mismatch repair

DNA mismatch repair (MMR) is a system for recognizing and repairing erroneous insertion, deletion, and mis-incorporation of bases that can arise during DNA replication and recombination, as well as repairing some forms of DNA damage.

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DNA polymerase

DNA polymerases are enzymes that synthesize DNA molecules from deoxyribonucleotides, the building blocks of DNA.

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DNA replication

In molecular biology, DNA replication is the biological process of producing two identical replicas of DNA from one original DNA molecule.

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Enzyme

Enzymes are macromolecular biological catalysts.

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Escherichia coli

Escherichia coli (also known as E. coli) is a Gram-negative, facultatively anaerobic, rod-shaped, coliform bacterium of the genus Escherichia that is commonly found in the lower intestine of warm-blooded organisms (endotherms).

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Eukaryote

Eukaryotes are organisms whose cells have a nucleus enclosed within membranes, unlike Prokaryotes (Bacteria and other Archaea).

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Genetic recombination

Genetic recombination (aka genetic reshuffling) is the production of offspring with combinations of traits that differ from those found in either parent.

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Immunofluorescence

Immunofluorescence is a technique used for light microscopy with a fluorescence microscope and is used primarily on microbiological samples.

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In vitro

In vitro (meaning: in the glass) studies are performed with microorganisms, cells, or biological molecules outside their normal biological context.

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Ligase

In biochemistry, a ligase is an enzyme that can catalyze the joining of two large molecules by forming a new chemical bond, usually with accompanying hydrolysis of a small pendant chemical group on one of the larger molecules or the enzyme catalyzing the linking together of two compounds, e.g., enzymes that catalyze joining of C-O, C-S, C-N, etc.

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Ligation (molecular biology)

In molecular biology, ligation is the joining of two nucleic acid fragments through the action of an enzyme.

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Nick translation

Nick translation (or head translation), developed in 1977 by Rigby and Paul Berg, is a tagging technique in molecular biology in which DNA Polymerase I is used to replace some of the nucleotides of a DNA sequence with their labeled analogues, creating a tagged DNA sequence which can be used as a probe in fluorescent in situ hybridization (FISH) or blotting techniques.

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Nicking enzyme

A nicking enzyme (or nicking endonuclease) is an enzyme that cuts one strand of a double-stranded DNA at a specific recognition nucleotide sequences known as a restriction site.

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Nicotinamide adenine dinucleotide

Nicotinamide adenine dinucleotide (NAD) is a coenzyme found in all living cells.

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Nucleic acid double helix

In molecular biology, the term double helix refers to the structure formed by double-stranded molecules of nucleic acids such as DNA.

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Nucleic acid structure

Nucleic acid structure refers to the structure of nucleic acids such as DNA and RNA.

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Nucleotide

Nucleotides are organic molecules that serve as the monomer units for forming the nucleic acid polymers deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), both of which are essential biomolecules within all life-forms on Earth.

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Okazaki fragments

Okazaki fragments are short, newly synthesized DNA fragments that are formed on the lagging template strand during DNA replication.

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Phosphodiester bond

A phosphodiester bond occurs when exactly two of the hydroxyl groups in phosphoric acid react with hydroxyl groups on other molecules to form two ester bonds.

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Ribonucleotide

In biochemistry, a ribonucleotide or ribotide is a nucleotide containing ribose as its pentose component.

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RNASEH2A

Ribonuclease H2 subunit A, also known as RNase H2 subunit A, is an enzyme that in humans is encoded by the RNASEH2A gene.

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Type I topoisomerase

In molecular biology Type I topoisomerases are enzymes that cut one of the two strands of double-stranded DNA, relax the strand, and reanneal the strand.

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Ultraviolet

Ultraviolet (UV) is electromagnetic radiation with a wavelength from 10 nm to 400 nm, shorter than that of visible light but longer than X-rays.

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References

[1] https://en.wikipedia.org/wiki/Nick_(DNA)

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