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96 relations: Acceleration, Ammonium sulfate, Amphoterism, Antibody, Antigen, Bicinchoninic acid assay, Biopharmaceutical, Bradford protein assay, C-terminus, Cell biology, Cell membrane, Centrifugal force, Centrifugation, Centrifuge, CHAPS detergent, Chemical structure, Chromatography, Cobalt, Coomassie Brilliant Blue, Denaturation (biochemistry), Deoxyribonuclease, Detergent, Dialysis, Differential centrifugation, DNA, Electrophoresis, ELISA, Elution, Enzyme, Freeze-drying, French pressure cell press, G-force, Gel electrophoresis, Gene expression, Glycoprotein, Gradient, High-performance liquid chromatography, Histidine, Hydrophobe, Imidazole, Immunoglobulin G, Immunoprecipitation, Inertia, Insulin, Ion, Lactase, Lectin, Ligand (biochemistry), Lysozyme, Membrane protein, ..., Metal, Metalloprotein, Metre, Mitochondrion, Molecular mass, Nickel, Oligosaccharide, Pepsin, Peptide mass fingerprinting, Percoll, PH, Polyhistidine-tag, Post-translational modification, Precipitation (chemistry), Protease, Protease inhibitor (biology), Protein, Protein A, Protein isoform, Protein production, Protein tag, Proteolysis, QPNC-PAGE, Radioligand, Receptor (biochemistry), Recombinant DNA, Reversed-phase chromatography, Salinity, Salting in, Salting out, SDS-PAGE, Size-exclusion chromatography, Sodium dodecyl sulfate, Solvent, Sonication, Soy protein, Spectroscopy, Strep-tag, Tissue (biology), Triton X-100, Two-dimensional gel electrophoresis, Ultracentrifuge, Ultrafiltration, Urease, Volatility (chemistry), Western blot. Expand index (46 more) »
Acceleration
In physics, acceleration is the rate of change of velocity of an object with respect to time.
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Ammonium sulfate
Ammonium sulfate (American English and international scientific usage; ammonium sulphate in British English); (NH4)2SO4, is an inorganic salt with a number of commercial uses.
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Amphoterism
In chemistry, an amphoteric compound is a molecule or ion that can react both as an acid as well as a base.
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Antibody
An antibody (Ab), also known as an immunoglobulin (Ig), is a large, Y-shaped protein produced mainly by plasma cells that is used by the immune system to neutralize pathogens such as pathogenic bacteria and viruses.
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Antigen
In immunology, an antigen is a molecule capable of inducing an immune response (to produce an antibody) in the host organism.
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Bicinchoninic acid assay
The bicinchoninic acid assay (BCA assay), also known as the Smith assay, after its inventor, Paul K. Smith at the Pierce Chemical Company, is a biochemical assay for determining the total concentration of protein in a solution (0.5 μg/mL to 1.5 mg/mL), similar to Lowry protein assay, Bradford protein assay or biuret reagent.
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Biopharmaceutical
A biopharmaceutical, also known as a biologic(al) medical product, biological, or biologic, is any pharmaceutical drug product manufactured in, extracted from, or semisynthesized from biological sources.
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Bradford protein assay
The Bradford protein assay was developed by Marion M. Bradford in 1976.
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C-terminus
The C-terminus (also known as the carboxyl-terminus, carboxy-terminus, C-terminal tail, C-terminal end, or COOH-terminus) is the end of an amino acid chain (protein or polypeptide), terminated by a free carboxyl group (-COOH).
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Cell biology
Cell biology (also called cytology, from the Greek κυτος, kytos, "vessel") is a branch of biology that studies the structure and function of the cell, the basic unit of life.
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Cell membrane
The cell membrane (also known as the plasma membrane or cytoplasmic membrane, and historically referred to as the plasmalemma) is a biological membrane that separates the interior of all cells from the outside environment (the extracellular space).
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Centrifugal force
In Newtonian mechanics, the centrifugal force is an inertial force (also called a "fictitious" or "pseudo" force) directed away from the axis of rotation that appears to act on all objects when viewed in a rotating frame of reference.
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Centrifugation
Centrifugation is a technique which involves the application of centrifugal force to separate particles from a solution according to their size, shape, density, viscosity of the medium and rotor speed.
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Centrifuge
A centrifuge is a piece of equipment that puts an object in rotation around a fixed axis (spins it in a circle), applying a force perpendicular to the axis of spin (outward) that can be very strong.
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CHAPS detergent
CHAPS is a zwitterionic surfactant used in the laboratory to solubilize biological macromolecules such as proteins.
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Chemical structure
A chemical structure determination includes a chemist's specifying the molecular geometry and, when feasible and necessary, the electronic structure of the target molecule or other solid.
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Chromatography
Chromatography is a laboratory technique for the separation of a mixture.
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Cobalt
Cobalt is a chemical element with symbol Co and atomic number 27.
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Coomassie Brilliant Blue
Coomassie Brilliant Blue is the name of two similar triphenylmethane dyes that were developed for use in the textile industry but are now commonly used for staining proteins in analytical biochemistry.
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Denaturation (biochemistry)
Denaturation is a process in which proteins or nucleic acids lose the quaternary structure, tertiary structure, and secondary structure which is present in their native state, by application of some external stress or compound such as a strong acid or base, a concentrated inorganic salt, an organic solvent (e.g., alcohol or chloroform), radiation or heat.
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Deoxyribonuclease
A deoxyribonuclease (DNase, for short) is an enzyme that catalyzes the hydrolytic cleavage of phosphodiester linkages in the DNA backbone, thus degrading DNA.
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Detergent
A detergent is a surfactant or a mixture of surfactants with cleaning properties in dilute solutions.
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Dialysis
In medicine, dialysis (from Greek διάλυσις, diàlysis, "dissolution"; from διά, dià, "through", and λύσις, lỳsis, "loosening or splitting") is the process of removing excess water, solutes and toxins from the blood in those whose native kidneys have lost the ability to perform these functions in a natural way.
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Differential centrifugation
Differential centrifugation is a common procedure in microbiology and cytology used to separate certain organelles from whole cells for further analysis of specific parts of cells.
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DNA
Deoxyribonucleic acid (DNA) is a thread-like chain of nucleotides carrying the genetic instructions used in the growth, development, functioning and reproduction of all known living organisms and many viruses.
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Electrophoresis
Electrophoresis (from the Greek "Ηλεκτροφόρηση" meaning "to bear electrons") is the motion of dispersed particles relative to a fluid under the influence of a spatially uniform electric field.
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ELISA
The enzyme-linked immunosorbent assay (ELISA) is a test that uses antibodies and color change to identify a substance.
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Elution
In analytical and organic chemistry, elution is the process of extracting one material from another by washing with a solvent; as in washing of loaded ion-exchange resins to remove captured ions.
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Enzyme
Enzymes are macromolecular biological catalysts.
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Freeze-drying
Freeze drying, also known as lyophilisation or cryodessication, is a low temperature dehydration process which involves freezing the product, lowering pressure, then removing the ice by sublimation.
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French pressure cell press
The French pressure cell press, or French press, is an apparatus used in biological experimentation to disrupt the plasma membrane of cells by passing them through a narrow valve under high pressure.
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G-force
The gravitational force, or more commonly, g-force, is a measurement of the type of acceleration that causes a perception of weight.
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Gel electrophoresis
Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge.
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Gene expression
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product.
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Glycoprotein
Glycoproteins are proteins that contain oligosaccharide chains (glycans) covalently attached to amino acid side-chains.
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Gradient
In mathematics, the gradient is a multi-variable generalization of the derivative.
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High-performance liquid chromatography
High-performance liquid chromatography (HPLC; formerly referred to as high-pressure liquid chromatography), is a technique in analytical chemistry used to separate, identify, and quantify each component in a mixture.
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Histidine
Histidine (symbol His or H) is an α-amino acid that is used in the biosynthesis of proteins.
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Hydrophobe
In chemistry, hydrophobicity is the physical property of a molecule (known as a hydrophobe) that is seemingly repelled from a mass of water.
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Imidazole
Imidazole is an organic compound with the formula C3N2H4.
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Immunoglobulin G
Immunoglobulin G (IgG) is a type of antibody.
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Immunoprecipitation
Immunoprecipitation (IP) is the technique of precipitating a protein antigen out of solution using an antibody that specifically binds to that particular protein.
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Inertia
Inertia is the resistance of any physical object to any change in its position and state of motion.
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Insulin
Insulin (from Latin insula, island) is a peptide hormone produced by beta cells of the pancreatic islets; it is considered to be the main anabolic hormone of the body.
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Ion
An ion is an atom or molecule that has a non-zero net electrical charge (its total number of electrons is not equal to its total number of protons).
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Lactase
Lactase is an enzyme produced by many organisms.
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Lectin
Lectins are carbohydrate-binding proteins, macromolecules that are highly specific for sugar moieties of other molecules.
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Ligand (biochemistry)
In biochemistry and pharmacology, a ligand is a substance that forms a complex with a biomolecule to serve a biological purpose.
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Lysozyme
Lysozyme, also known as muramidase or N-acetylmuramide glycanhydrolase is an antimicrobial enzyme produced by animals that forms part of the innate immune system.
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Membrane protein
Membrane proteins are proteins that interact with, or are part of, biological membranes.
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Metal
A metal (from Greek μέταλλον métallon, "mine, quarry, metal") is a material (an element, compound, or alloy) that is typically hard when in solid state, opaque, shiny, and has good electrical and thermal conductivity.
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Metalloprotein
Metalloprotein is a generic term for a protein that contains a metal ion cofactor.
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Metre
The metre (British spelling and BIPM spelling) or meter (American spelling) (from the French unit mètre, from the Greek noun μέτρον, "measure") is the base unit of length in some metric systems, including the International System of Units (SI).
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Mitochondrion
The mitochondrion (plural mitochondria) is a double-membrane-bound organelle found in most eukaryotic organisms.
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Molecular mass
Relative Molecular mass or molecular weight is the mass of a molecule.
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Nickel
Nickel is a chemical element with symbol Ni and atomic number 28.
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Oligosaccharide
An oligosaccharide (from the Greek ὀλίγος olígos, "a few", and σάκχαρ sácchar, "sugar") is a saccharide polymer containing a small number (typically three to ten) of monosaccharides (simple sugars).
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Pepsin
Pepsin is an endopeptidase that breaks down proteins into smaller peptides (that is, a protease).
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Peptide mass fingerprinting
Peptide mass fingerprinting (PMF) (also known as protein fingerprinting) is an analytical technique for protein identification in which the unknown protein of interest is first cleaved into smaller peptides, whose absolute masses can be accurately measured with a mass spectrometer such as MALDI-TOF or ESI-TOF.
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Percoll
Percoll is a tool for more efficient density separation in biochemistry that was first formulated by Pertoft and colleagues.
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PH
In chemistry, pH is a logarithmic scale used to specify the acidity or basicity of an aqueous solution.
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Polyhistidine-tag
A polyhistidine-tag is an amino acid motif in proteins that consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein.
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Post-translational modification
Post-translational modification (PTM) refers to the covalent and generally enzymatic modification of proteins following protein biosynthesis.
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Precipitation (chemistry)
Precipitation is the creation of a solid from a solution.
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Protease
A protease (also called a peptidase or proteinase) is an enzyme that performs proteolysis: protein catabolism by hydrolysis of peptide bonds.
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Protease inhibitor (biology)
In biology and biochemistry, protease inhibitors are molecules that inhibit the function of proteases (enzymes that aid the breakdown of proteins).
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Protein
Proteins are large biomolecules, or macromolecules, consisting of one or more long chains of amino acid residues.
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Protein A
Protein A is a 42 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus.
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Protein isoform
A protein isoform, or "protein variant" is a member of a set of highly similar proteins that originate from a single gene or gene family and are the result of genetic differences.
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Protein production
Protein production is the biotechnological process of generating a specific protein.
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Protein tag
Protein tags are peptide sequences genetically grafted onto a recombinant protein.
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Proteolysis
Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids.
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QPNC-PAGE
QPNC-PAGE, or quantitative preparative native continuous polyacrylamide gel electrophoresis, is a bioanalytical, high-resolution and highly accurate technique applied in biochemistry and bioinorganic chemistry to separate proteins quantitatively by isoelectric point.
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Radioligand
A radioligand is a radioactive biochemical substance (in particular, a ligand that is radiolabeled) that is used for diagnosis or for research-oriented study of the receptor systems of the body.
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Receptor (biochemistry)
In biochemistry and pharmacology, a receptor is a protein molecule that receives chemical signals from outside a cell.
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Recombinant DNA
Recombinant DNA (rDNA) molecules are DNA molecules formed by laboratory methods of genetic recombination (such as molecular cloning) to bring together genetic material from multiple sources, creating sequences that would not otherwise be found in the genome.
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Reversed-phase chromatography
Reversed-phase chromatography (also called RPC, reverse-phase chromatography, or hydrophobic chromatography) includes any chromatographic method that uses a hydrophobic stationary phase.
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Salinity
Salinity is the saltiness or amount of salt dissolved in a body of water (see also soil salinity).
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Salting in
Salting in refers to the effect where increasing the ionic strength of a solution increases the solubility of some solute (such as a protein).
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Salting out
Salting out (also known as salt-induced precipitation, salt fractionation, anti-solvent crystallization, precipitation crystallization, or drowning out) is an effect based on the electrolyte-non electrolyte interaction, in which the non-electrolyte could be less soluble at high salt concentrations.
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SDS-PAGE
SDS-PAGE (sodium dodecyl sulfate–polyacrylamide gel electrophoresis) is a variant of polyacrylamide gel electrophoresis, an analytical method in biochemistry for the separation of charged molecules in mixtures by their molecular masses in an electric field.
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Size-exclusion chromatography
Size-exclusion chromatography (SEC), also known as molecular sieve chromatography, is a chromatographic method in which molecules in solution are separated by their size, and in some cases molecular weight.
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Sodium dodecyl sulfate
Sodium dodecyl sulfate (SDS), synonymously sodium lauryl sulfate (SLS), or sodium laurilsulfate, is a synthetic organic compound with the formula CH3(CH2)11SO4 Na.
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Solvent
A solvent (from the Latin solvō, "loosen, untie, solve") is a substance that dissolves a solute (a chemically distinct liquid, solid or gas), resulting in a solution.
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Sonication
A sonicator at the Weizmann Institute of Science during sonicationSonication is the act of applying sound energy to agitate particles in a sample, for various purposes such as the extraction of multiple compounds from plants, microalgae and seaweeds.
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Soy protein
Soy protein is a protein that is isolated from soybean.
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Spectroscopy
Spectroscopy is the study of the interaction between matter and electromagnetic radiation.
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Strep-tag
The Strep-tag® system is a method which allows the purification and detection of proteins by affinity chromatography.
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Tissue (biology)
In biology, tissue is a cellular organizational level between cells and a complete organ.
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Triton X-100
Triton X-100 (n) is a nonionic surfactant that has a hydrophilic polyethylene oxide chain (on average it has 9.5 ethylene oxide units) and an aromatic hydrocarbon lipophilic or hydrophobic group.
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Two-dimensional gel electrophoresis
Two-dimensional gel electrophoresis, abbreviated as 2-DE or 2-D electrophoresis, is a form of gel electrophoresis commonly used to analyze proteins.
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Ultracentrifuge
The ultracentrifuge is a centrifuge optimized for spinning a rotor at very high speeds, capable of generating acceleration as high as (approx.). There are two kinds of ultracentrifuges, the preparative and the analytical ultracentrifuge.
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Ultrafiltration
Ultrafiltration (UF) is a variety of membrane filtration in which forces like pressure or concentration gradients lead to a separation through a semipermeable membrane.
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Urease
Ureases, functionally, belong to the superfamily of amidohydrolases and phosphotriesterases.
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Volatility (chemistry)
In chemistry and physics, volatility is quantified by the tendency of a substance to vaporize.
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Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used in molecular biology, immunogenetics and other molecular biology disciplines to detect specific proteins in a sample of tissue homogenate or extract.
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Redirects here:
Protein isolate, Protein isolation.
References
[1] https://en.wikipedia.org/wiki/Protein_purification
