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Q-FISH

Index Q-FISH

Quantitative Fluorescent in situ hybridization (Q-FISH) is a cytogenetic technique based on the traditional FISH methodology. [1]

49 relations: Bromodeoxyuridine, Cancer, Centromere, Chromosome, Chromosome abnormality, Current Protocols, Cyanine, Cytogenetics, DAPI, Demecolcine, Denaturation (biochemistry), DNA, DNA repair, Fixation (histology), Flow cytometry, Flow-FISH, Fluorescein isothiocyanate, Fluorescence in situ hybridization, Fluorescence microscope, Fluorophore, Formaldehyde, Gel electrophoresis, Genome instability, Gram stain, Interphase, Karyotype, Ku70, Ku80, Locus (genetics), Metaphase, Microtubule, Mitosis, Non-homologous end joining, Oligonucleotide, Pepsin, Peptide nucleic acid, Phosphate-buffered saline, Photobleaching, Plasmid, Protease, Protein dimer, Restriction enzyme, Ribosomal RNA, Southern blot, Telomere, Tissue culture, Tonicity, Trypsin, Vertebrate.

Bromodeoxyuridine

Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd, broxuridine) is a synthetic nucleoside that is an analog of thymidine.

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Cancer

Cancer is a group of diseases involving abnormal cell growth with the potential to invade or spread to other parts of the body.

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Centromere

The centromere is the specialized DNA sequence of a chromosome that links a pair of sister chromatids (a dyad).

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Chromosome

A chromosome (from Ancient Greek: χρωμόσωμα, chromosoma, chroma means colour, soma means body) is a DNA molecule with part or all of the genetic material (genome) of an organism.

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Chromosome abnormality

A chromosome abnormality, disorder, anomaly, aberration, or mutation is a missing, extra, or irregular portion of chromosomal DNA.

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Current Protocols

Current Protocols is a series of laboratory manuals for life scientists.

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Cyanine

Cyanine is the non-systematic name of a synthetic dye family belonging to polymethine group.

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Cytogenetics

Cytogenetics is a branch of genetics that is concerned with how the chromosomes relate to cell behaviour, particularly to their behaviour during mitosis and meiosis.

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DAPI

DAPI, or 4′,6-diamidino-2-phenylindole, is a fluorescent stain that binds strongly to adenine–thymine rich regions in DNA.

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Demecolcine

Demecolcine (INN; also known as colcemid) is a drug used in chemotherapy.

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Denaturation (biochemistry)

Denaturation is a process in which proteins or nucleic acids lose the quaternary structure, tertiary structure, and secondary structure which is present in their native state, by application of some external stress or compound such as a strong acid or base, a concentrated inorganic salt, an organic solvent (e.g., alcohol or chloroform), radiation or heat.

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DNA

Deoxyribonucleic acid (DNA) is a thread-like chain of nucleotides carrying the genetic instructions used in the growth, development, functioning and reproduction of all known living organisms and many viruses.

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DNA repair

DNA repair is a collection of processes by which a cell identifies and corrects damage to the DNA molecules that encode its genome.

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Fixation (histology)

In the fields of histology, pathology, and cell biology, fixation is the preservation of biological tissues from decay due to autolysis or putrefaction.

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Flow cytometry

In biotechnology, flow cytometry is a laser- or impedance-based, biophysical technology employed in cell counting, cell sorting, biomarker detection and protein engineering, by suspending cells in a stream of fluid and passing them through an electronic detection apparatus.

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Flow-FISH

Flow-FISH (fluorescent in-situ hybridization) is a cytogenetic technique to quantify the copy number of specific repetitive elements in genomic DNA of whole cell populations via the combination of flow cytometry with cytogenetic fluorescent in situ hybridization staining protocols.

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Fluorescein isothiocyanate

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry.

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Fluorescence in situ hybridization

Fluorescent in situ hybridization (FISH) is a molecular cytogenetic technique that uses fluorescent probes that bind to only those parts of the chromosome with a high degree of sequence complementarity.

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Fluorescence microscope

A fluorescence microscope is an optical microscope that uses fluorescence and phosphorescence instead of, or in addition to, reflection and absorption to study properties of organic or inorganic substances.

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Fluorophore

A fluorophore (or fluorochrome, similarly to a chromophore) is a fluorescent chemical compound that can re-emit light upon light excitation.

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Formaldehyde

No description.

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Gel electrophoresis

Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge.

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Genome instability

Genome instability (also genetic instability or genomic instability) refers to a high frequency of mutations within the genome of a cellular lineage.

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Gram stain

Gram stain or Gram staining, also called Gram's method, is a method of staining used to distinguish and classify bacterial species into two large groups (gram-positive and gram-negative).

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Interphase

Interphase is the phase of the cell cycle in which a typical cell spends most of its life.

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Karyotype

A karyotype is the number and appearance of chromosomes in the nucleus of a eukaryotic cell.

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Ku70

Ku70 is a protein that, in humans, is encoded by the XRCC6 gene.

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Ku80

Ku80 is a protein that, in humans, is encoded by the XRCC5 gene.

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Locus (genetics)

A locus (plural loci) in genetics is a fixed position on a chromosome, like the position of a gene or a marker (genetic marker).

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Metaphase

Metaphase (from the Greek μετά, "adjacent" and φάσις, "stage") is a stage of mitosis in the eukaryotic cell cycle in which chromosomes are at their second-most condensed and coiled stage (they are at their most condensed in anaphase).

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Microtubule

Microtubules are tubular polymers of tubulin that form part of the cytoskeleton that provides the cytoplasm of eukaryotic cells and some bacteria with structure and shape.

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Mitosis

In cell biology, mitosis is a part of the cell cycle when replicated chromosomes are separated into two new nuclei.

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Non-homologous end joining

Non-homologous end joining (NHEJ) is a pathway that repairs double-strand breaks in DNA.

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Oligonucleotide

Oligonucleotides are short DNA or RNA molecules, oligomers, that have a wide range of applications in genetic testing, research, and forensics.

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Pepsin

Pepsin is an endopeptidase that breaks down proteins into smaller peptides (that is, a protease).

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Peptide nucleic acid

Peptide nucleic acid (PNA) is an artificially synthesized polymer similar to DNA or RNA.

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Phosphate-buffered saline

Phosphate-buffered saline (abbreviated PBS) is a buffer solution commonly used in biological research.

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Photobleaching

In optics, photobleaching (sometimes termed fading) is the photochemical alteration of a dye or a fluorophore molecule such that it permanently is unable to fluoresce.

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Plasmid

A plasmid is a small DNA molecule within a cell that is physically separated from a chromosomal DNA and can replicate independently.

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Protease

A protease (also called a peptidase or proteinase) is an enzyme that performs proteolysis: protein catabolism by hydrolysis of peptide bonds.

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Protein dimer

In biochemistry, a protein dimer is a macromolecular complex formed by two protein monomers, or single proteins, which are usually non-covalently bound.

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Restriction enzyme

A restriction enzyme or restriction endonuclease is an enzyme that cleaves DNA into fragments at or near specific recognition sites within the molecule known as restriction sites.

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Ribosomal RNA

Ribosomal ribonucleic acid (rRNA) is the RNA component of the ribosome, and is essential for protein synthesis in all living organisms.

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Southern blot

A Southern blot is a method used in molecular biology for detection of a specific DNA sequence in DNA samples.

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Telomere

A telomere is a region of repetitive nucleotide sequences at each end of a chromosome, which protects the end of the chromosome from deterioration or from fusion with neighboring chromosomes.

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Tissue culture

Tissue culture is the growth of tissues or cells separate from the organism.

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Tonicity

Tonicity is a measure of the effective osmotic pressure gradient, as defined by the water potential of two solutions separated by a semipermeable membrane.

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Trypsin

Trypsin is a serine protease from the PA clan superfamily, found in the digestive system of many vertebrates, where it hydrolyzes proteins.

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Vertebrate

Vertebrates comprise all species of animals within the subphylum Vertebrata (chordates with backbones).

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References

[1] https://en.wikipedia.org/wiki/Q-FISH

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