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Virtual colony count

Index Virtual colony count

Virtual colony count (VCC) is a kinetic, 96-well microbiological assay originally developed to measure the activity of defensins. [1]

58 relations: Aerosol, Alanine, Alanine scanning, Alpha defensin, Amino acid, Anthrax, Antimicrobial, Antimicrobial peptides, Assay, ATCC (company), Bacillus anthracis, Bacillus cereus, Bacteria, Bacterial growth, Bactericide, Bacteriostatic agent, Beta bulge, Beta defensin, Bioaerosol, Biofilm, Biosafety, Biosafety cabinet, Buffer solution, Cell (biology), Cell membrane, Cell wall, DEFA1, DEFA4, DEFA5, DEFA6, Defensin, Electrostatics, Enantiomer, Enterobacter aerogenes, Escherichia coli, Exponential growth, Fission (biology), Glycine, Gram-negative bacteria, Gram-positive bacteria, Hydrophobe, LL37, Microbiological culture, Microbiology, Microtiter plate, Minimum inhibitory concentration, Mutagenesis, Mutation, Pathogen, Pipette, ..., Plate reader, Protein dimer, Protein precursor, Quality control, Real-time polymerase chain reaction, Staphylococcus aureus, Theta defensin, Turbidity. Expand index (8 more) »

Aerosol

An aerosol is a suspension of fine solid particles or liquid droplets, in air or another gas.

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Alanine

Alanine (symbol Ala or A) is an α-amino acid that is used in the biosynthesis of proteins.

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Alanine scanning

In molecular biology, alanine scanning is a technique used to determine the contribution of a specific residue to the stability or function of given protein.

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Alpha defensin

Alpha defensins are a family of mammalian defensin peptides.

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Amino acid

Amino acids are organic compounds containing amine (-NH2) and carboxyl (-COOH) functional groups, along with a side chain (R group) specific to each amino acid.

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Anthrax

Anthrax is an infection caused by the bacterium Bacillus anthracis.

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Antimicrobial

An antimicrobial is an agent that kills microorganisms or stops their growth.

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Antimicrobial peptides

Antimicrobial peptides (AMPs), also called host defense peptides (HDPs) are part of the innate immune response found among all classes of life.

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Assay

An assay is an investigative (analytic) procedure in laboratory medicine, pharmacology, environmental biology and molecular biology for qualitatively assessing or quantitatively measuring the presence, amount, or functional activity of a target entity (the analyte).

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ATCC (company)

ATCC or the American Type Culture Collection is a nonprofit organization which collects, stores, and distributes standard reference microorganisms, cell lines and other materials for research and development.

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Bacillus anthracis

Bacillus anthracis is the etiologic agent of anthrax—a common disease of livestock and, occasionally, of humans—and the only obligate pathogen within the genus Bacillus.

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Bacillus cereus

Bacillus cereus is a Gram-positive, rod-shaped, aerobic, facultatively anaerobic, motile, beta hemolytic bacterium commonly found in soil and food.

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Bacteria

Bacteria (common noun bacteria, singular bacterium) is a type of biological cell.

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Bacterial growth

Growth is shown as ''L''.

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Bactericide

A bactericide or bacteriocide, sometimes abbreviated Bcidal, is a substance that kills bacteria.

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Bacteriostatic agent

A bacteriostatic agent or bacteriostat, abbreviated Bstatic, is a biological or chemical agent that stops bacteria from reproducing, while not necessarily killing them otherwise.

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Beta bulge

A beta bulge can be described as a localized disruption of the regular hydrogen bonding of beta sheet by inserting extra residues into one or both hydrogen bonded β-strands.

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Beta defensin

Beta defensins are a family of mammalian defensins.

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Bioaerosol

Bioaerosols (short for biological aerosols) are a subcategory of particles released from terrestrial and marine ecosystems into the atmosphere.

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Biofilm

A biofilm comprises any group of microorganisms in which cells stick to each other and often also to a surface.

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Biosafety

Biosafety is the prevention of large-scale loss of biological integrity, focusing both on ecology and human health.

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Biosafety cabinet

A biosafety cabinet (BSC)—also called a biological safety cabinet or microbiological safety cabinet—is an enclosed, ventilated laboratory workspace for safely working with materials contaminated with (or potentially contaminated with) pathogens requiring a defined biosafety level.

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Buffer solution

A buffer solution (more precisely, pH buffer or hydrogen ion buffer) is an aqueous solution consisting of a mixture of a weak acid and its conjugate base, or vice versa.

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Cell (biology)

The cell (from Latin cella, meaning "small room") is the basic structural, functional, and biological unit of all known living organisms.

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Cell membrane

The cell membrane (also known as the plasma membrane or cytoplasmic membrane, and historically referred to as the plasmalemma) is a biological membrane that separates the interior of all cells from the outside environment (the extracellular space).

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Cell wall

A cell wall is a structural layer surrounding some types of cells, just outside the cell membrane.

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DEFA1

Defensin, alpha 1 also known as human alpha defensin 1, human neutrophil peptide 1 (HNP-1) or neutrophil defensin 1 is a human protein that is encoded by the DEFA1 gene.

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DEFA4

Defensin, alpha 4 (DEFA4), also known as neutrophil defensin 4 or HNP4, is a human defensin peptide that is encoded by the DEFA4 gene.

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DEFA5

Defensin, alpha 5 (DEFA5) also known as human alpha defensin 5 (HD5) is a human protein that is encoded by the DEFA5 gene.

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DEFA6

Defensin, alpha 6 (DEFA6) also known as human alpha defensin 6 (HD6) is a human protein that is encoded by the DEFA6 gene.

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Defensin

Defensins are small cysteine-rich cationic proteins found in both vertebrates and invertebrates.

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Electrostatics

Electrostatics is a branch of physics that studies electric charges at rest.

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Enantiomer

In chemistry, an enantiomer, also known as an optical isomer (and archaically termed antipode or optical antipode), is one of two stereoisomers that are mirror images of each other that are non-superposable (not identical), much as one's left and right hands are the same except for being reversed along one axis (the hands cannot be made to appear identical simply by reorientation).

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Enterobacter aerogenes

Klebsiella aerogenes, previously known as Enterobacter aerogenes, is a Gram-negative, oxidase negative, catalase positive, citrate positive, indole negative, rod-shaped bacterium.

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Escherichia coli

Escherichia coli (also known as E. coli) is a Gram-negative, facultatively anaerobic, rod-shaped, coliform bacterium of the genus Escherichia that is commonly found in the lower intestine of warm-blooded organisms (endotherms).

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Exponential growth

Exponential growth is exhibited when the rate of change—the change per instant or unit of time—of the value of a mathematical function is proportional to the function's current value, resulting in its value at any time being an exponential function of time, i.e., a function in which the time value is the exponent.

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Fission (biology)

Fission, in biology, is the division of a single entity into two or more parts and the regeneration of those parts into separate entities resembling the original.

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Glycine

Glycine (symbol Gly or G) is the amino acid that has a single hydrogen atom as its side chain.

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Gram-negative bacteria

Gram-negative bacteria are bacteria that do not retain the crystal violet stain used in the gram-staining method of bacterial differentiation.

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Gram-positive bacteria

Gram-positive bacteria are bacteria that give a positive result in the Gram stain test, which is traditionally used to quickly classify bacteria into two broad categories according to their cell wall.

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Hydrophobe

In chemistry, hydrophobicity is the physical property of a molecule (known as a hydrophobe) that is seemingly repelled from a mass of water.

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LL37

LL-37 (or CAP-18 for cathelicidin antimicrobial peptide, 18 kDa) is a gene encoding for the only member of the human cathelicidin family.

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Microbiological culture

A microbiological culture, or microbial culture, is a method of multiplying microbial organisms by letting them reproduce in predetermined culture medium under controlled laboratory conditions.

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Microbiology

Microbiology (from Greek μῑκρος, mīkros, "small"; βίος, bios, "life"; and -λογία, -logia) is the study of microorganisms, those being unicellular (single cell), multicellular (cell colony), or acellular (lacking cells).

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Microtiter plate

A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or microwell plate or multiwell, is a flat plate with multiple "wells" used as small test tubes.

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Minimum inhibitory concentration

In, the minimum inhibitory concentration (MIC) is the lowest concentration of a chemical which prevents visible growth of a bacterium.

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Mutagenesis

Mutagenesis is a process by which the genetic information of an organism is changed, resulting in a mutation.

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Mutation

In biology, a mutation is the permanent alteration of the nucleotide sequence of the genome of an organism, virus, or extrachromosomal DNA or other genetic elements.

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Pathogen

In biology, a pathogen (πάθος pathos "suffering, passion" and -γενής -genēs "producer of") or a '''germ''' in the oldest and broadest sense is anything that can produce disease; the term came into use in the 1880s.

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Pipette

A pipette (sometimes spelled pipet) is a laboratory tool commonly used in chemistry, biology and medicine to transport a measured volume of liquid, often as a media dispenser.

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Plate reader

Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates.

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Protein dimer

In biochemistry, a protein dimer is a macromolecular complex formed by two protein monomers, or single proteins, which are usually non-covalently bound.

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Protein precursor

A protein precursor, also called a pro-protein or pro-peptide, is an inactive protein (or peptide) that can be turned into an active form by post-translational modification, such as breaking off a piece of the molecule or adding on another molecule.

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Quality control

Quality control, or QC for short, is a process by which entities review the quality of all factors involved in production.

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Real-time polymerase chain reaction

A real-time polymerase chain reaction (Real-Time PCR), also known as quantitative polymerase chain reaction (qPCR), is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR).

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Staphylococcus aureus

Staphylococcus aureus is a Gram-positive, round-shaped bacterium that is a member of the Firmicutes, and it is a member of the normal flora of the body, frequently found in the nose, respiratory tract, and on the skin.

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Theta defensin

Theta-defensins (θ-defensins or retrocyclins) are a family of mammalian antimicrobial peptides.

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Turbidity

Turbidity is the cloudiness or haziness of a fluid caused by large numbers of individual particles that are generally invisible to the naked eye, similar to smoke in air.

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References

[1] https://en.wikipedia.org/wiki/Virtual_colony_count

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