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Reversed-phase chromatography

Index Reversed-phase chromatography

Reversed-phase chromatography (also called RPC, reverse-phase chromatography, or hydrophobic chromatography) includes any chromatographic method that uses a hydrophobic stationary phase. [1]

17 relations: Acetonitrile, Adsorption, Alkyl, Aluminium oxide, Aqueous normal-phase chromatography, Aqueous solution, Chemical polarity, Chromatography, Elution, Endcapping, High-performance liquid chromatography, Hydrophile, Hydrophobe, Ion interaction chromatography, Resolution (mass spectrometry), Silicon dioxide, Tetrahydrofuran.


Acetonitrile is the chemical compound with the formula.

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Adsorption is the adhesion of atoms, ions or molecules from a gas, liquid or dissolved solid to a surface.

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In organic chemistry, an alkyl substituent is an alkane missing one hydrogen.

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Aluminium oxide

Aluminium oxide (British English) or aluminum oxide (American English) is a chemical compound of aluminium and oxygen with the chemical formula 23.

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Aqueous normal-phase chromatography

Aqueous normal-phase chromatography (ANP) is a chromatographic technique which involves the mobile phase region between reversed-phase chromatography (RP) and organic normal-phase chromatography (ONP).

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Aqueous solution

An aqueous solution is a solution in which the solvent is water.

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Chemical polarity

In chemistry, polarity is a separation of electric charge leading to a molecule or its chemical groups having an electric dipole or multipole moment.

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Chromatography is a laboratory technique for the separation of a mixture.

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In analytical and organic chemistry, elution is the process of extracting one material from another by washing with a solvent; as in washing of loaded ion-exchange resins to remove captured ions.

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In chromatography, endcapping refers to the replacement of accessible silanol groups in a bonded stationary phase by trimethylsilyl groups.

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High-performance liquid chromatography

High-performance liquid chromatography (HPLC; formerly referred to as high-pressure liquid chromatography), is a technique in analytical chemistry used to separate, identify, and quantify each component in a mixture.

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A hydrophile is a molecule or other molecular entity that is attracted to water molecules and tends to be dissolved by water.

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In chemistry, hydrophobicity is the physical property of a molecule (known as a hydrophobe) that is seemingly repelled from a mass of water.

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Ion interaction chromatography

Ion interaction chromatography (ion-pair chromatography) is a laboratory technique for separating ions with chromatography.

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Resolution (mass spectrometry)

In mass spectrometry, resolution measures of the ability to distinguish two peaks of slightly different mass-to-charge ratios ΔM, in a mass spectrum.

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Silicon dioxide

Silicon dioxide, also known as silica (from the Latin silex), is an oxide of silicon with the chemical formula, most commonly found in nature as quartz and in various living organisms.

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Tetrahydrofuran (THF) is an organic compound with the formula (CH2)4O.

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Redirects here:

Reverse phase chromatography, Reverse phase column, Reversed-phase liquid chromatography.


[1] https://en.wikipedia.org/wiki/Reversed-phase_chromatography

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