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44 relations: Alkali, Antibody, Archaea, Autoradiograph, Bacteria, Base pair, Complementarity (molecular biology), Cycling probe technology, Denaturation (biochemistry), Digoxigenin, DNA, DNA profiling, Eukaryote, Fluorescence in situ hybridization, In vivo, Isotope, Isotopes of phosphorus, Locked nucleic acid, Microarray, Microbial ecology, Microsatellite, Molecular beacon, Molecular biology, Molecular cloning, Molecular marker, Morpholino, Northern blot, Nucleic acid, Nucleic acid hybridization, Nucleic acid methods, Nucleotide, Oligonucleotide synthesis, Phosphodiester bond, Phosphoramidite, Phosphorus, Polymerase chain reaction, Radioactive decay, Radioactive tracer, Restriction fragment length polymorphism, RNA, Sodium hydroxide, Southern blot, TaqMan, 16S ribosomal RNA.
Alkali
In chemistry, an alkali (from Arabic: al-qaly “ashes of the saltwort”) is a basic, ionic salt of an alkali metal or alkaline earth metal chemical element.
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Antibody
An antibody (Ab), also known as an immunoglobulin (Ig), is a large, Y-shaped protein produced mainly by plasma cells that is used by the immune system to neutralize pathogens such as pathogenic bacteria and viruses.
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Archaea
Archaea (or or) constitute a domain of single-celled microorganisms.
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Autoradiograph
An autoradiograph is an image on an x-ray film or nuclear emulsion produced by the pattern of decay emissions (e.g., beta particles or gamma rays) from a distribution of a radioactive substance.
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Bacteria
Bacteria (common noun bacteria, singular bacterium) is a type of biological cell.
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Base pair
A base pair (bp) is a unit consisting of two nucleobases bound to each other by hydrogen bonds.
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Complementarity (molecular biology)
In molecular biology, complementarity describes a relationship between two structures each following the lock-and-key principle.
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Cycling probe technology
Cycling probe technology (CPT) is a molecular biological technique for detecting specific DNA sequences.
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Denaturation (biochemistry)
Denaturation is a process in which proteins or nucleic acids lose the quaternary structure, tertiary structure, and secondary structure which is present in their native state, by application of some external stress or compound such as a strong acid or base, a concentrated inorganic salt, an organic solvent (e.g., alcohol or chloroform), radiation or heat.
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Digoxigenin
Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea, Digitalis orientalis and Digitalis lanata (foxgloves), where it is attached to sugars, to form the glycosides (e.g. Lanatoside C).
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DNA
Deoxyribonucleic acid (DNA) is a thread-like chain of nucleotides carrying the genetic instructions used in the growth, development, functioning and reproduction of all known living organisms and many viruses.
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DNA profiling
DNA profiling (also called DNA fingerprinting, DNA testing, or DNA typing) is the process of determining an individual's DNA characteristics, which are as unique as fingerprints.
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Eukaryote
Eukaryotes are organisms whose cells have a nucleus enclosed within membranes, unlike Prokaryotes (Bacteria and other Archaea).
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Fluorescence in situ hybridization
Fluorescent in situ hybridization (FISH) is a molecular cytogenetic technique that uses fluorescent probes that bind to only those parts of the chromosome with a high degree of sequence complementarity.
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In vivo
Studies that are in vivo (Latin for "within the living"; often not italicized in English) are those in which the effects of various biological entities are tested on whole, living organisms or cells, usually animals, including humans, and plants, as opposed to a tissue extract or dead organism.
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Isotope
Isotopes are variants of a particular chemical element which differ in neutron number.
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Isotopes of phosphorus
Although phosphorus (15P) has 23 isotopes from 24P to 46P, only one of these isotopes is stable 31P; as such, it is considered a monoisotopic element.
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Locked nucleic acid
A locked nucleic acid (LNA), often referred to as inaccessible RNA, is a modified RNA nucleotide.
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Microarray
A microarray is a multiplex lab-on-a-chip.
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Microbial ecology
Microbial ecology (or environmental microbiology) is the ecology of microorganisms: their relationship with one another and with their environment.
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Microsatellite
A microsatellite is a tract of repetitive DNA in which certain DNA motifs (ranging in length from 1–6 or more base pairs) are repeated, typically 5–50 times.
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Molecular beacon
Molecular beacons are oligonucleotide hybridization probes that can report the presence of specific nucleic acids in homogenous solutions.
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Molecular biology
Molecular biology is a branch of biology which concerns the molecular basis of biological activity between biomolecules in the various systems of a cell, including the interactions between DNA, RNA, proteins and their biosynthesis, as well as the regulation of these interactions.
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Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms.
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Molecular marker
A molecular marker is a molecule contained within a sample taken from an organism (biological markers) or other matter.
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Morpholino
A Morpholino, also known as a Morpholino oligomer and as a phosphorodiamidate Morpholino oligomer (PMO), is a type of oligomer molecule (colloquially, an oligo) used in molecular biology to modify gene expression.
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Northern blot
The northern blot, or RNA blot,Gilbert, S. F. (2000) Developmental Biology, 6th Ed.
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Nucleic acid
Nucleic acids are biopolymers, or small biomolecules, essential to all known forms of life.
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Nucleic acid hybridization
In molecular biology, hybridization (or hybridisation) is a phenomenon in which single-stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) molecules anneal to complementary DNA or RNA.
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Nucleic acid methods
Nucleic acid methods are the techniques used to study nucleic acids: DNA and RNA.
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Nucleotide
Nucleotides are organic molecules that serve as the monomer units for forming the nucleic acid polymers deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), both of which are essential biomolecules within all life-forms on Earth.
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Oligonucleotide synthesis
Oligonucleotide synthesis is the chemical synthesis of relatively short fragments of nucleic acids with defined chemical structure (sequence).
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Phosphodiester bond
A phosphodiester bond occurs when exactly two of the hydroxyl groups in phosphoric acid react with hydroxyl groups on other molecules to form two ester bonds.
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Phosphoramidite
A phosphoramidite (RO)2PNR2 is a monoamide of a phosphite diester.
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Phosphorus
Phosphorus is a chemical element with symbol P and atomic number 15.
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Polymerase chain reaction
Polymerase chain reaction (PCR) is a technique used in molecular biology to amplify a single copy or a few copies of a segment of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence.
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Radioactive decay
Radioactive decay (also known as nuclear decay or radioactivity) is the process by which an unstable atomic nucleus loses energy (in terms of mass in its rest frame) by emitting radiation, such as an alpha particle, beta particle with neutrino or only a neutrino in the case of electron capture, gamma ray, or electron in the case of internal conversion.
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Radioactive tracer
A radioactive tracer, or radioactive label, is a chemical compound in which one or more atoms have been replaced by a radionuclide so by virtue of its radioactive decay it can be used to explore the mechanism of chemical reactions by tracing the path that the radioisotope follows from reactants to products.
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Restriction fragment length polymorphism
In molecular biology, restriction fragment length polymorphism (RFLP) is a technique that exploits variations in homologous DNA sequences.
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RNA
Ribonucleic acid (RNA) is a polymeric molecule essential in various biological roles in coding, decoding, regulation, and expression of genes.
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Sodium hydroxide
Sodium hydroxide, also known as lye, is an inorganic compound with the formula NaOH. It is a white solid ionic compound consisting of sodium cations and hydroxide anions. Sodium hydroxide is a highly caustic base and alkali that decomposes proteins at ordinary ambient temperatures and may cause severe chemical burns. It is highly soluble in water, and readily absorbs moisture and carbon dioxide from the air. It forms a series of hydrates NaOH·n. The monohydrate NaOH· crystallizes from water solutions between 12.3 and 61.8 °C. The commercially available "sodium hydroxide" is often this monohydrate, and published data may refer to it instead of the anhydrous compound. As one of the simplest hydroxides, it is frequently utilized alongside neutral water and acidic hydrochloric acid to demonstrate the pH scale to chemistry students. Sodium hydroxide is used in many industries: in the manufacture of pulp and paper, textiles, drinking water, soaps and detergents, and as a drain cleaner. Worldwide production in 2004 was approximately 60 million tonnes, while demand was 51 million tonnes.
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Southern blot
A Southern blot is a method used in molecular biology for detection of a specific DNA sequence in DNA samples.
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TaqMan
TaqMan probes are hydrolysis probes that are designed to increase the specificity of quantitative PCR.
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16S ribosomal RNA
16S ribosomal RNA (or 16S rRNA) is the component of the 30S small subunit of a prokaryotic ribosome that binds to the Shine-Dalgarno sequence.
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References
[1] https://en.wikipedia.org/wiki/Hybridization_probe
