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Micrococcal nuclease

Index Micrococcal nuclease

Micrococcal nuclease (S7 Nuclease, MNase, spleen endonuclease, thermonuclease, nuclease T, micrococcal endonuclease, nuclease T, staphylococcal nuclease, spleen phosphodiesterase, Staphylococcus aureus nuclease, Staphylococcus aureus nuclease B, ribonucleate (deoxynucleate) 3'-nucleotidohydrolase) is an endo-exonuclease that preferentially digests single-stranded nucleic acids. [1]

28 relations: Alpha helix, Base pair, Beta sheet, Chromatin, CUT&RUN sequencing, DNA, EGTA (chemical), Endonuclease, Enzyme, Escherichia coli, Exonuclease, Gene, Molecular mass, Nuclease, Nucleic acid, Oligonucleotide, PH, Phosphate, Protein, Protein Data Bank, Protein folding, Reticulocyte, Ribbon diagram, RNA, Staphylococcus, Staphylococcus aureus, Structural Classification of Proteins database, X-ray crystallography.

Alpha helix

The alpha helix (α-helix) is a common motif in the secondary structure of proteins and is a righthand-spiral conformation (i.e. helix) in which every backbone N−H group donates a hydrogen bond to the backbone C.

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Base pair

A base pair (bp) is a unit consisting of two nucleobases bound to each other by hydrogen bonds.

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Beta sheet

The β-sheet (also β-pleated sheet) is a common motif of regular secondary structure in proteins.

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Chromatin

Chromatin is a complex of macromolecules found in cells, consisting of DNA, protein, and RNA.

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CUT&RUN sequencing

CUT&RUN-sequencing, also known as Cleavage Under Targets and Release Using Nuclease, is a method used to analyze protein interactions with DNA.

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DNA

Deoxyribonucleic acid (DNA) is a thread-like chain of nucleotides carrying the genetic instructions used in the growth, development, functioning and reproduction of all known living organisms and many viruses.

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EGTA (chemical)

EGTA (ethylene glycol-bis(β-aminoethyl ether)-N,N,N',N'-tetraacetic acid), also known as egtazic acid (INN, USAN), is an aminopolycarboxylic acid, a chelating agent.

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Endonuclease

Endonucleases are enzymes that cleave the phosphodiester bond within a polynucleotide chain.

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Enzyme

Enzymes are macromolecular biological catalysts.

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Escherichia coli

Escherichia coli (also known as E. coli) is a Gram-negative, facultatively anaerobic, rod-shaped, coliform bacterium of the genus Escherichia that is commonly found in the lower intestine of warm-blooded organisms (endotherms).

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Exonuclease

Exonucleases are enzymes that work by cleaving nucleotides one at a time from the end (exo) of a polynucleotide chain.

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Gene

In biology, a gene is a sequence of DNA or RNA that codes for a molecule that has a function.

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Molecular mass

Relative Molecular mass or molecular weight is the mass of a molecule.

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Nuclease

A nuclease (also archaically known as nucleodepolymerase or polynucleotidase) is an enzyme capable of cleaving the phosphodiester bonds between monomers of nucleic acids.

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Nucleic acid

Nucleic acids are biopolymers, or small biomolecules, essential to all known forms of life.

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Oligonucleotide

Oligonucleotides are short DNA or RNA molecules, oligomers, that have a wide range of applications in genetic testing, research, and forensics.

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PH

In chemistry, pH is a logarithmic scale used to specify the acidity or basicity of an aqueous solution.

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Phosphate

A phosphate is chemical derivative of phosphoric acid.

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Protein

Proteins are large biomolecules, or macromolecules, consisting of one or more long chains of amino acid residues.

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Protein Data Bank

The Protein Data Bank (PDB) is a crystallographic database for the three-dimensional structural data of large biological molecules, such as proteins and nucleic acids.

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Protein folding

Protein folding is the physical process by which a protein chain acquires its native 3-dimensional structure, a conformation that is usually biologically functional, in an expeditious and reproducible manner.

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Reticulocyte

Reticulocytes are immature red blood cells, typically composing about 1% of the red blood cells in the human body.

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Ribbon diagram

Ribbon diagrams, also known as Richardson diagrams, are 3D schematic representations of protein structure and are one of the most common methods of protein depiction used today.

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RNA

Ribonucleic acid (RNA) is a polymeric molecule essential in various biological roles in coding, decoding, regulation, and expression of genes.

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Staphylococcus

Staphylococcus (from the σταφυλή, staphylē, "grape" and κόκκος, kókkos, "granule") is a genus of Gram-positive bacteria.

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Staphylococcus aureus

Staphylococcus aureus is a Gram-positive, round-shaped bacterium that is a member of the Firmicutes, and it is a member of the normal flora of the body, frequently found in the nose, respiratory tract, and on the skin.

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Structural Classification of Proteins database

The Structural Classification of Proteins (SCOP) database is a largely manual classification of protein structural domains based on similarities of their structures and amino acid sequences.

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X-ray crystallography

X-ray crystallography is a technique used for determining the atomic and molecular structure of a crystal, in which the crystalline atoms cause a beam of incident X-rays to diffract into many specific directions.

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Redirects here:

EC 3.1.31.1, EC 3.1.4.7, MNase, Micrococcal endonuclease, Nuclease T, Nuclease T', Ribonucleate (deoxynucleate) 3'-nucleotidohydrolase, S7 Nuclease, Spleen endonuclease, Staphylococcal nuclease, Staphylococcus aureus nuclease, Staphylococcus aureus nuclease B, Thermonuclease.

References

[1] https://en.wikipedia.org/wiki/Micrococcal_nuclease

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