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Terminal restriction fragment length polymorphism

Index Terminal restriction fragment length polymorphism

Terminal restriction fragment length polymorphism (TRFLP or sometimes T-RFLP) is a molecular biology technique for profiling of microbial communities based on the position of a restriction site closest to a labelled end of an amplified gene. [1]

22 relations: Amplicon, Amplified Ribosomal DNA Restriction Analysis, Cluster analysis, Community fingerprinting, DNA sequencer, Electropherogram, Electrophoresis, Exonuclease, Fluorescence, Fluorophore, Library (biology), Molecular biology, Phospholipid-derived fatty acids, Polymerase chain reaction, Restriction enzyme, Restriction fragment length polymorphism, Restriction site, Rhodamine, Ribosomal intergenic spacer analysis, Temperature gradient gel electrophoresis, 16S ribosomal RNA, 6-Carboxyfluorescein.

Amplicon

In molecular biology, an amplicon is a piece of DNA or RNA that is the source and/or product of amplification or replication events.

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Amplified Ribosomal DNA Restriction Analysis

Amplified rDNA (Ribosomal DNA) Restriction Analysis is the extension of the technique of RFLP (restriction fragment length polymorphism) to the gene encoding the small (16s) ribosomal subunit of bacteria.

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Cluster analysis

Cluster analysis or clustering is the task of grouping a set of objects in such a way that objects in the same group (called a cluster) are more similar (in some sense) to each other than to those in other groups (clusters).

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Community fingerprinting

Community fingerprinting is a set of molecular biology techniques that can be used to quickly profile the diversity of a microbial community.

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DNA sequencer

A DNA sequencer is a scientific instrument used to automate the DNA sequencing process.

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Electropherogram

An electropherogram (or electrophorogram) is a record or chart produced when electrophoresis is used in an analytical technique, primarily in the fields of molecular biology or biochemistry.

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Electrophoresis

Electrophoresis (from the Greek "Ηλεκτροφόρηση" meaning "to bear electrons") is the motion of dispersed particles relative to a fluid under the influence of a spatially uniform electric field.

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Exonuclease

Exonucleases are enzymes that work by cleaving nucleotides one at a time from the end (exo) of a polynucleotide chain.

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Fluorescence

Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation.

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Fluorophore

A fluorophore (or fluorochrome, similarly to a chromophore) is a fluorescent chemical compound that can re-emit light upon light excitation.

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Library (biology)

In molecular biology, a library is a collection of DNA fragments that is stored and propagated in a population of micro-organisms through the process of molecular cloning.

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Molecular biology

Molecular biology is a branch of biology which concerns the molecular basis of biological activity between biomolecules in the various systems of a cell, including the interactions between DNA, RNA, proteins and their biosynthesis, as well as the regulation of these interactions.

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Phospholipid-derived fatty acids

Phospholipid-derived fatty acids (PLFA) are widely used in microbial ecology as chemotaxonomic markers of bacteria and other organisms.

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Polymerase chain reaction

Polymerase chain reaction (PCR) is a technique used in molecular biology to amplify a single copy or a few copies of a segment of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence.

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Restriction enzyme

A restriction enzyme or restriction endonuclease is an enzyme that cleaves DNA into fragments at or near specific recognition sites within the molecule known as restriction sites.

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Restriction fragment length polymorphism

In molecular biology, restriction fragment length polymorphism (RFLP) is a technique that exploits variations in homologous DNA sequences.

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Restriction site

Restriction sites, or restriction recognition sites, are locations on a DNA molecule containing specific (4-8 base pairs in length) sequences of nucleotides, which are recognized by restriction enzymes.

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Rhodamine

Rhodamine is a family of related chemical compounds, fluorone dyes.

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Ribosomal intergenic spacer analysis

Ribosomal RNA (rRNA) intergenic spacer analysis (RISA) is a method of microbial community analysis that provides a means of comparing differing environments or treatment impacts without the bias imposed by culture- dependent approaches.

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Temperature gradient gel electrophoresis

Temperature gradient gel electrophoresis (TGGE) and denaturing gradient gel electrophoresis (DGGE) are forms of electrophoresis which use either a temperature or chemical gradient to denature the sample as it moves across an acrylamide gel.

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16S ribosomal RNA

16S ribosomal RNA (or 16S rRNA) is the component of the 30S small subunit of a prokaryotic ribosome that binds to the Shine-Dalgarno sequence.

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6-Carboxyfluorescein

6-Carboxyfluorescein (6-FAM) is a fluorescent dye with an absorption wavelength of 495 nm and an emission wavelength of 517 nm.

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Redirects here:

T-RFLP, TRFLP, Terminal Restriction Fragment Length Polymorphism.

References

[1] https://en.wikipedia.org/wiki/Terminal_restriction_fragment_length_polymorphism

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