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Temperature gradient gel electrophoresis

Index Temperature gradient gel electrophoresis

Temperature gradient gel electrophoresis (TGGE) and denaturing gradient gel electrophoresis (DGGE) are forms of electrophoresis which use either a temperature or chemical gradient to denature the sample as it moves across an acrylamide gel. [1]

35 relations: Acrylamide, Adenine, Archaea, Bacteria, Base pair, Buffer solution, Carcinoma, Community fingerprinting, Cytosine, Denaturation (biochemistry), DNA, Electrophoresis, Elution, Eukaryote, Genome, Guanine, Heteroduplex, Laboratory of Molecular Biology, Leiden University, Leonard Lerman, Mitochondrial DNA, Mutation, Nucleic acid, Nucleic acid thermodynamics, P53, Pancreas, Parallel (geometry), Perpendicular, Polymerase chain reaction, Polymorphism (biology), Protein, Ribosome, RNA, Roger Wartell, Thymine.

Acrylamide

Acrylamide (or acrylic amide) is a chemical compound with the chemical formula C3H5NO.

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Adenine

Adenine (A, Ade) is a nucleobase (a purine derivative).

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Archaea

Archaea (or or) constitute a domain of single-celled microorganisms.

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Bacteria

Bacteria (common noun bacteria, singular bacterium) is a type of biological cell.

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Base pair

A base pair (bp) is a unit consisting of two nucleobases bound to each other by hydrogen bonds.

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Buffer solution

A buffer solution (more precisely, pH buffer or hydrogen ion buffer) is an aqueous solution consisting of a mixture of a weak acid and its conjugate base, or vice versa.

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Carcinoma

Carcinoma is a type of cancer that develops from epithelial cells.

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Community fingerprinting

Community fingerprinting is a set of molecular biology techniques that can be used to quickly profile the diversity of a microbial community.

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Cytosine

Cytosine (C) is one of the four main bases found in DNA and RNA, along with adenine, guanine, and thymine (uracil in RNA).

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Denaturation (biochemistry)

Denaturation is a process in which proteins or nucleic acids lose the quaternary structure, tertiary structure, and secondary structure which is present in their native state, by application of some external stress or compound such as a strong acid or base, a concentrated inorganic salt, an organic solvent (e.g., alcohol or chloroform), radiation or heat.

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DNA

Deoxyribonucleic acid (DNA) is a thread-like chain of nucleotides carrying the genetic instructions used in the growth, development, functioning and reproduction of all known living organisms and many viruses.

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Electrophoresis

Electrophoresis (from the Greek "Ηλεκτροφόρηση" meaning "to bear electrons") is the motion of dispersed particles relative to a fluid under the influence of a spatially uniform electric field.

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Elution

In analytical and organic chemistry, elution is the process of extracting one material from another by washing with a solvent; as in washing of loaded ion-exchange resins to remove captured ions.

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Eukaryote

Eukaryotes are organisms whose cells have a nucleus enclosed within membranes, unlike Prokaryotes (Bacteria and other Archaea).

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Genome

In the fields of molecular biology and genetics, a genome is the genetic material of an organism.

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Guanine

Guanine (or G, Gua) is one of the four main nucleobases found in the nucleic acids DNA and RNA, the others being adenine, cytosine, and thymine (uracil in RNA).

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Heteroduplex

A heteroduplex is a double-stranded (duplex) molecule of nucleic acid originated through the genetic recombination of single complementary strands derived from different sources, such as from different homologous chromosomes or even from different organisms.

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Laboratory of Molecular Biology

The Medical Research Council (MRC) Laboratory of Molecular Biology (LMB) is a research institute in Cambridge, England, involved in the revolution in molecular biology which occurred in the 1950–60s, since then it remains a major medical research laboratory with a much broader focus.

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Leiden University

Leiden University (abbreviated as LEI; Universiteit Leiden), founded in the city of Leiden, is the oldest university in the Netherlands.

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Leonard Lerman

Leonard Solomon Lerman (June 27, 1925 – September 19, 2012) was an American scientist most noted for his work on DNA.

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Mitochondrial DNA

Mitochondrial DNA (mtDNA or mDNA) is the DNA located in mitochondria, cellular organelles within eukaryotic cells that convert chemical energy from food into a form that cells can use, adenosine triphosphate (ATP).

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Mutation

In biology, a mutation is the permanent alteration of the nucleotide sequence of the genome of an organism, virus, or extrachromosomal DNA or other genetic elements.

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Nucleic acid

Nucleic acids are biopolymers, or small biomolecules, essential to all known forms of life.

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Nucleic acid thermodynamics

Nucleic acid thermodynamics is the study of how temperature affects the nucleic acid structure of double-stranded DNA (dsDNA).

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P53

Tumor protein p53, also known as p53, cellular tumor antigen p53 (UniProt name), phosphoprotein p53, tumor suppressor p53, antigen NY-CO-13, or transformation-related protein 53 (TRP53), is any isoform of a protein encoded by homologous genes in various organisms, such as TP53 (humans) and Trp53 (mice).

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Pancreas

The pancreas is a glandular organ in the digestive system and endocrine system of vertebrates.

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Parallel (geometry)

In geometry, parallel lines are lines in a plane which do not meet; that is, two lines in a plane that do not intersect or touch each other at any point are said to be parallel.

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Perpendicular

In elementary geometry, the property of being perpendicular (perpendicularity) is the relationship between two lines which meet at a right angle (90 degrees).

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Polymerase chain reaction

Polymerase chain reaction (PCR) is a technique used in molecular biology to amplify a single copy or a few copies of a segment of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence.

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Polymorphism (biology)

Polymorphism in biology and zoology is the occurrence of two or more clearly different morphs or forms, also referred to as alternative phenotypes, in the population of a species.

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Protein

Proteins are large biomolecules, or macromolecules, consisting of one or more long chains of amino acid residues.

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Ribosome

The ribosome is a complex molecular machine, found within all living cells, that serves as the site of biological protein synthesis (translation).

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RNA

Ribonucleic acid (RNA) is a polymeric molecule essential in various biological roles in coding, decoding, regulation, and expression of genes.

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Roger Wartell

Roger Martin Wartell is the former Chair of the School of Biology, part of the College of Sciences at the Georgia Institute of Technology.

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Thymine

---> Thymine (T, Thy) is one of the four nucleobases in the nucleic acid of DNA that are represented by the letters G–C–A–T.

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Redirects here:

DGGE, Denaturing gradient gel electrophoresis, Dgge, TGGE, Temperature Gradient Gel Electrophoresis, Tgge.

References

[1] https://en.wikipedia.org/wiki/Temperature_gradient_gel_electrophoresis

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