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Transmission electron cryomicroscopy

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Transmission electron cryomicroscopy (CryoTEM) is a form of cryogenic electron microscopy, more specifically a type of transmission electron microscopy (TEM) where the sample is studied at cryogenic temperatures (generally liquid-nitrogen temperatures). [1]

40 relations: Adenoviridae, Amorphous ice, Ångström, CMOS, Covalent bond, Cryofixation, Cryogenic electron microscopy, Cryogenics, Crystal, Dehydration, Electron cryotomography, Electron crystallography, Electron microscope, EM Data Bank, Ethane, European Molecular Biology Laboratory, Helix, Ion channel, Jacques Dubochet, Joachim Frank, Liquid nitrogen, Mitochondrion, Negative stain, Nobel Prize in Chemistry, Noise (electronics), Oxford University Press, Radiation damage, Radiosensitivity, Resolution (electron density), Ribosome, Richard Henderson (biologist), Scanning electron cryomicroscopy, Single particle analysis, Structural biology, Transmembrane protein, Transmission electron microscopy, Unified atomic mass unit, Vacuum, Virus, X-ray crystallography.

Adenoviridae

Adenoviruses (members of the family Adenoviridae) are medium-sized (90–100 nm), nonenveloped (without an outer lipid bilayer) viruses with an icosahedral nucleocapsid containing a double stranded DNA genome.

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Amorphous ice

Amorphous ice (non-crystalline ("vitreous") ice) is an amorphous solid form of water.

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Ångström

The ångström or angstrom is a unit of length equal to (one ten-billionth of a metre) or 0.1 nanometre.

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CMOS

Complementary metal–oxide–semiconductor, abbreviated as CMOS, is a technology for constructing integrated circuits.

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Covalent bond

A covalent bond, also called a molecular bond, is a chemical bond that involves the sharing of electron pairs between atoms.

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Cryofixation

Cryofixation is a technique for fixation or stabilisation of biological materials as the first step in specimen preparation for electron microscopy and cryo-electron microscopy.

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Cryogenic electron microscopy

Electron cryomicroscopy (CryoEM) is an electron microscopy (EM) technique where the sample is cooled to cryogenic temperatures.

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Cryogenics

In physics, cryogenics is the production and behaviour of materials at very low temperatures.

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Crystal

A crystal or crystalline solid is a solid material whose constituents (such as atoms, molecules, or ions) are arranged in a highly ordered microscopic structure, forming a crystal lattice that extends in all directions.

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Dehydration

In physiology, dehydration is a deficit of total body water, with an accompanying disruption of metabolic processes.

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Electron cryotomography

Electron cryotomography (CryoET) is an imaging technique used to produce high-resolution (~4 nm) three-dimensional views of samples, typically biological macromolecules and cells.

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Electron crystallography

Electron crystallography is a method to determine the arrangement of atoms in solids using a transmission electron microscope (TEM).

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Electron microscope

An electron microscope is a microscope that uses a beam of accelerated electrons as a source of illumination.

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EM Data Bank

The EM Data Bank or Electron Microscopy Data Bank (EMDB) collects 3D EM maps and associated experimental data determined using electron microscopy of biological specimens.

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Ethane

Ethane is an organic chemical compound with chemical formula.

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European Molecular Biology Laboratory

The European Molecular Biology Laboratory (EMBL) is a molecular biology research institution supported by 25 member states, four prospect and two associate member states.

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Helix

A helix, plural helixes or helices, is a type of smooth space curve, i.e. a curve in three-dimensional space.

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Ion channel

Ion channels are pore-forming membrane proteins that allow ions to pass through the channel pore.

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Jacques Dubochet

Jacques Dubochet (born 8 June 1942) is a retired Swiss biophysicist.

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Joachim Frank

Joachim Frank (born 12 September 1940) is a German-born American biophysicist at Columbia University and a Nobel laureate.

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Liquid nitrogen

Liquid nitrogen is nitrogen in a liquid state at an extremely low temperature.

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Mitochondrion

The mitochondrion (plural mitochondria) is a double-membrane-bound organelle found in most eukaryotic organisms.

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Negative stain

Negative staining is an established method, often used in diagnostic microscopy, for contrasting a thin specimen with an optically opaque fluid.

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Nobel Prize in Chemistry

The Nobel Prize in Chemistry (Nobelpriset i kemi) is awarded annually by the Royal Swedish Academy of Sciences to scientists in the various fields of chemistry.

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Noise (electronics)

In electronics, noise is an unwanted disturbance in an electrical signal.

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Oxford University Press

Oxford University Press (OUP) is the largest university press in the world, and the second oldest after Cambridge University Press.

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Radiation damage

This article deals with Radiation damage due to the effects of ionizing radiation on physical objects.

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Radiosensitivity

Radiosensitivity is the relative susceptibility of cells, tissues, organs or organisms to the harmful effect of ionizing radiation.

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Resolution (electron density)

Resolution in terms of electron density is a measure of the resolvability in the electron density map of a molecule.

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Ribosome

The ribosome is a complex molecular machine, found within all living cells, that serves as the site of biological protein synthesis (translation).

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Richard Henderson (biologist)

Richard Henderson, CH, FRS, FMedSci, HonFRSC (born 19 July 1945) is a Scottish molecular biologist and biophysicist and pioneer in the field of electron microscopy of biological molecules.

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Scanning electron cryomicroscopy

Scanning electron cryomicroscopy (CryoSEM) is a form of electron microscopy where a hydrated but cryogenically fixed sample is imaged on a scanning electron microscope's cold stage in a cryogenic chamber.

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Single particle analysis

Single particle analysis is a group of related computerized image processing techniques used to analyze images from transmission electron microscopy (TEM).

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Structural biology

Structural biology is a branch of molecular biology, biochemistry, and biophysics concerned with the molecular structure of biological macromolecules (especially proteins, made up of amino acids, and RNA or DNA, made up of nucleic acids), how they acquire the structures they have, and how alterations in their structures affect their function.

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Transmembrane protein

A transmembrane protein (TP) is a type of integral membrane protein that spans the entirety of the biological membrane to which it is permanently attached.

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Transmission electron microscopy

Transmission electron microscopy (TEM, also sometimes conventional transmission electron microscopy or CTEM) is a microscopy technique in which a beam of electrons is transmitted through a specimen to form an image.

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Unified atomic mass unit

The unified atomic mass unit or dalton (symbol: u, or Da) is a standard unit of mass that quantifies mass on an atomic or molecular scale (atomic mass).

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Vacuum

Vacuum is space devoid of matter.

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Virus

A virus is a small infectious agent that replicates only inside the living cells of other organisms.

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X-ray crystallography

X-ray crystallography is a technique used for determining the atomic and molecular structure of a crystal, in which the crystalline atoms cause a beam of incident X-rays to diffract into many specific directions.

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Redirects here:

3DEM, 3dem, CEMOVIS, Cryo em, Cryo-EM study, Cryo-TEM, Cryo-transmission electron microscopy, CryoTEM, Cryoelectron Microscopy, Cryoelectron microscopy, Cryogenic transmission electron microscopy, Cyrogenic-Electron Microscopy (Cryo-EM), Electron Cryomicroscopy, Electron Cyromicroscopy, Electron cryo-microscopy, Molecular microscopy, Software tools for molecular microscopy.

References

[1] https://en.wikipedia.org/wiki/Transmission_electron_cryomicroscopy

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