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Two-dimensional gel electrophoresis

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Two-dimensional gel electrophoresis, abbreviated as 2-DE or 2-D electrophoresis, is a form of gel electrophoresis commonly used to analyze proteins. [1]

25 relations: Benjamin Cravatt III, Cell (biology), Chloroquine, Coomassie Brilliant Blue, Difference gel electrophoresis, DNA, DNA supercoil, Electrophoresis, Ethidium bromide, Gel electrophoresis, Immobilized pH gradient, In-gel digestion, Isoelectric focusing, Isoelectric point, Mass, Missing data, Organelle, Polyacrylamide gel electrophoresis, Protein complex, Protein mass spectrometry, QPNC-PAGE, Quantitative proteomics, SDS-PAGE, Silver stain, Sodium dodecyl sulfate.

Benjamin Cravatt III

Benjamin Franklin Cravatt III is a professor in and chair of the Department of Chemical Physiology at The Scripps Research Institute in La Jolla, California.

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Cell (biology)

The cell (from Latin cella, meaning "small room") is the basic structural, functional, and biological unit of all known living organisms.

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Chloroquine

Chloroquine is a medication used to prevent and to treat malaria in areas where malaria is known to be sensitive to its effects.

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Coomassie Brilliant Blue

Coomassie Brilliant Blue is the name of two similar triphenylmethane dyes that were developed for use in the textile industry but are now commonly used for staining proteins in analytical biochemistry.

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Difference gel electrophoresis

Difference gel electrophoresis (DIGE) is a form of gel electrophoresis where up to three different protein samples can be labeled with size-matched, charge-matched spectrally resolvable fluorescent dyes (for example Cy3, Cy5, Cy2) prior to two dimensional gel electrophoresis.

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DNA

Deoxyribonucleic acid (DNA) is a thread-like chain of nucleotides carrying the genetic instructions used in the growth, development, functioning and reproduction of all known living organisms and many viruses.

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DNA supercoil

DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand.

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Electrophoresis

Electrophoresis (from the Greek "Ηλεκτροφόρηση" meaning "to bear electrons") is the motion of dispersed particles relative to a fluid under the influence of a spatially uniform electric field.

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Ethidium bromide

Ethidium bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis.

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Gel electrophoresis

Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge.

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Immobilized pH gradient

Immobilized pH gradient (IPG) gels are the acrylamide gel matrix co-polymerized with the pH gradient, which result in completely stable gradients except the most alkaline (>12) pH values.

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In-gel digestion

The in-gel digestion is part of the sample preparation for the mass spectrometric identification of proteins in course of proteomic analysis.

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Isoelectric focusing

Isoelectric focusing (IEF), also known as electrofocusing, is a technique for separating different molecules by differences in their isoelectric point (pI).

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Isoelectric point

The isoelectric point (pI, pH(I), IEP), is the pH at which a particular molecule carries no net electrical charge or is electrically neutral in the statistical mean.

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Mass

Mass is both a property of a physical body and a measure of its resistance to acceleration (a change in its state of motion) when a net force is applied.

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Missing data

In statistics, missing data, or missing values, occur when no data value is stored for the variable in an observation.

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Organelle

In cell biology, an organelle is a specialized subunit within a cell that has a specific function, in which their function is vital for the cell to live.

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Polyacrylamide gel electrophoresis

Polyacrylamide gel electrophoresis (PAGE) is a technique widely used in biochemistry, forensic chemistry, genetics, molecular biology and biotechnology to separate biological macromolecules, usually proteins or nucleic acids, according to their electrophoretic mobility.

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Protein complex

A protein complex or multiprotein complex is a group of two or more associated polypeptide chains.

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Protein mass spectrometry

Protein mass spectrometry refers to the application of mass spectrometry to the study of proteins.

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QPNC-PAGE

QPNC-PAGE, or quantitative preparative native continuous polyacrylamide gel electrophoresis, is a bioanalytical, high-resolution and highly accurate technique applied in biochemistry and bioinorganic chemistry to separate proteins quantitatively by isoelectric point.

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Quantitative proteomics

Quantitative proteomics is an analytical chemistry technique for determining the amount of proteins in a sample.

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SDS-PAGE

SDS-PAGE (sodium dodecyl sulfate–polyacrylamide gel electrophoresis) is a variant of polyacrylamide gel electrophoresis, an analytical method in biochemistry for the separation of charged molecules in mixtures by their molecular masses in an electric field.

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Silver stain

Silver staining is the use of silver to selectively alter the appearance of a target in microscopy of histological sections; in temperature gradient gel electrophoresis; and in polyacrylamide gels.

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Sodium dodecyl sulfate

Sodium dodecyl sulfate (SDS), synonymously sodium lauryl sulfate (SLS), or sodium laurilsulfate, is a synthetic organic compound with the formula CH3(CH2)11SO4 Na.

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References

[1] https://en.wikipedia.org/wiki/Two-dimensional_gel_electrophoresis

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