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66 relations: AH109, Amino acid, Biomolecular structure, Biosynthesis, Bombyx mori, California sea hare, Candida albicans, CDNA library, Cytokine receptor, DNA, DNA ligase, DNA methyltransferase, DNA sequencing, DNA-binding protein, EGR1, Escherichia coli, Eukaryote, Gal4 transcription factor, Gene ontology, Genetic engineering, Glycoprotein 130, Green fluorescent protein, Helper virus, High-throughput screening, Immunoprecipitation, Integral membrane protein, Isopropyl β-D-1-thiogalactopyranoside, Kinase, Lac operon, Lac repressor, Library (biology), Mammal, Mass spectrometry, MCherry, Molecular biology, Network topology, Nuclear localization sequence, Nucleic acid, Phage display, Phosphorylation, Plasmid, Protease, Protein, Protein domain, Protein engineering, Protein microarray, Protein-fragment complementation assay, Protein–protein interaction, Reporter gene, Restriction enzyme, ..., Reverse transcriptase, Saccharomyces cerevisiae, Stanley Fields (biologist), STAT3, Synthetic genetic array, Takara Holdings, TetR, Transcription (biology), Transcription factor, Tyrosine kinase 2, Ubiquitin, Upstream activating sequence, Upstream and downstream (DNA), Y187, Zinc finger, 3-Amino-1,2,4-triazole. Expand index (16 more) »
AH109
AH109 is a strain of yeast (Saccharomyces cerevisiae) used in biological research for two-hybrid screening.
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Amino acid
Amino acids are organic compounds containing amine (-NH2) and carboxyl (-COOH) functional groups, along with a side chain (R group) specific to each amino acid.
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Biomolecular structure
Biomolecular structure is the intricate folded, three-dimensional shape that is formed by a molecule of protein, DNA, or RNA, and that is important to its function.
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Biosynthesis
Biosynthesis (also called anabolism) is a multi-step, enzyme-catalyzed process where substrates are converted into more complex products in living organisms.
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Bombyx mori
The silkworm is the larva or caterpillar or imago of the domestic silkmoth, Bombyx mori (Latin: "silkworm of the mulberry tree").
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California sea hare
The California sea hare, scientific name Aplysia californica, is a species of sea slug in the sea hare family, Aplysiidae.
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Candida albicans
Candida albicans is an opportunistic pathogenic yeast that is a common member of the human gut flora.
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CDNA library
A cDNA library is a combination of cloned cDNA (complementary DNA) fragments inserted into a collection of host cells, which together constitute some portion of the transcriptome of the organism and are stored as a "library".
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Cytokine receptor
Cytokine receptors are receptors that bind cytokines.
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DNA
Deoxyribonucleic acid (DNA) is a thread-like chain of nucleotides carrying the genetic instructions used in the growth, development, functioning and reproduction of all known living organisms and many viruses.
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DNA ligase
DNA ligase is a specific type of enzyme, a ligase, that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond.
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DNA methyltransferase
In biochemistry, the DNA methyltransferase (DNA MTase) family of enzymes catalyze the transfer of a methyl group to DNA.
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DNA sequencing
DNA sequencing is the process of determining the precise order of nucleotides within a DNA molecule.
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DNA-binding protein
DNA-binding proteins are proteins that have DNA-binding domains and thus have a specific or general affinity for single- or double-stranded DNA.
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EGR1
EGR-1 (Early growth response protein 1) also known as Zif268 (zinc finger protein 225) or NGFI-A (nerve growth factor-induced protein A) is a protein that in humans is encoded by the EGR1 gene.
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Escherichia coli
Escherichia coli (also known as E. coli) is a Gram-negative, facultatively anaerobic, rod-shaped, coliform bacterium of the genus Escherichia that is commonly found in the lower intestine of warm-blooded organisms (endotherms).
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Eukaryote
Eukaryotes are organisms whose cells have a nucleus enclosed within membranes, unlike Prokaryotes (Bacteria and other Archaea).
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Gal4 transcription factor
The Gal4 transcription factor is a positive regulator of gene expression of galactose-induced genes.
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Gene ontology
Gene ontology (GO) is a major bioinformatics initiative to unify the representation of gene and gene product attributes across all species.
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Genetic engineering
Genetic engineering, also called genetic modification or genetic manipulation, is the direct manipulation of an organism's genes using biotechnology.
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Glycoprotein 130
Glycoprotein 130 (also known as gp130, IL6ST, IL6-beta or CD130) is a transmembrane protein which is the founding member of the class of all cytokine receptors.
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Green fluorescent protein
The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range.
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Helper virus
A helper virus is a virus used when producing copies of a helper dependent viral vector which does not have the ability to replicate on its own.
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High-throughput screening
High-throughput screening (HTS) is a method for scientific experimentation especially used in drug discovery and relevant to the fields of biology and chemistry.
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Immunoprecipitation
Immunoprecipitation (IP) is the technique of precipitating a protein antigen out of solution using an antibody that specifically binds to that particular protein.
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Integral membrane protein
An integral membrane protein (IMP) is a type of membrane protein that is permanently attached to the biological membrane.
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Isopropyl β-D-1-thiogalactopyranoside
Isopropyl β-D-1-thiogalactopyranoside (IPTG) is a molecular biology reagent.
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Kinase
In biochemistry, a kinase is an enzyme that catalyzes the transfer of phosphate groups from high-energy, phosphate-donating molecules to specific substrates.
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Lac operon
The lac operon (lactose operon) is an operon required for the transport and metabolism of lactose in Escherichia coli and many other enteric bacteria.
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Lac repressor
The lac repressor is a DNA-binding protein which inhibits the expression of genes coding for proteins involved in the metabolism of lactose in bacteria.
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Library (biology)
In molecular biology, a library is a collection of DNA fragments that is stored and propagated in a population of micro-organisms through the process of molecular cloning.
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Mammal
Mammals are the vertebrates within the class Mammalia (from Latin mamma "breast"), a clade of endothermic amniotes distinguished from reptiles (including birds) by the possession of a neocortex (a region of the brain), hair, three middle ear bones, and mammary glands.
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Mass spectrometry
Mass spectrometry (MS) is an analytical technique that ionizes chemical species and sorts the ions based on their mass-to-charge ratio.
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MCherry
mCherry is a fluorophore (a fluorescent protein) used as a marker when tagged to molecules and cell components.
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Molecular biology
Molecular biology is a branch of biology which concerns the molecular basis of biological activity between biomolecules in the various systems of a cell, including the interactions between DNA, RNA, proteins and their biosynthesis, as well as the regulation of these interactions.
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Network topology
Network topology is the arrangement of the elements (links, nodes, etc.) of a communication network.
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Nuclear localization sequence
A nuclear localization signal or sequence (NLS) is an amino acid sequence that 'tags' a protein for import into the cell nucleus by nuclear transport.
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Nucleic acid
Nucleic acids are biopolymers, or small biomolecules, essential to all known forms of life.
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Phage display
Phage display is a laboratory technique for the study of protein–protein, protein–peptide, and protein–DNA interactions that uses bacteriophages (viruses that infect bacteria) to connect proteins with the genetic information that encodes them.
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Phosphorylation
In chemistry, phosphorylation of a molecule is the attachment of a phosphoryl group.
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Plasmid
A plasmid is a small DNA molecule within a cell that is physically separated from a chromosomal DNA and can replicate independently.
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Protease
A protease (also called a peptidase or proteinase) is an enzyme that performs proteolysis: protein catabolism by hydrolysis of peptide bonds.
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Protein
Proteins are large biomolecules, or macromolecules, consisting of one or more long chains of amino acid residues.
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Protein domain
A protein domain is a conserved part of a given protein sequence and (tertiary) structure that can evolve, function, and exist independently of the rest of the protein chain.
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Protein engineering
Protein engineering is the process of developing useful or valuable proteins.
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Protein microarray
A protein microarray (or protein chip) is a high-throughput method used to track the interactions and activities of proteins, and to determine their function, and determining function on a large scale.
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Protein-fragment complementation assay
Within the field of molecular biology, a protein-fragment complementation assay, or PCA, is a method for the identification and quantification of protein–protein interactions.
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Protein–protein interaction
Protein–protein interactions (PPIs) are the physical contacts of high specificity established between two or more protein molecules as a result of biochemical events steered by electrostatic forces including the hydrophobic effect.
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Reporter gene
In molecular biology, a reporter gene (often simply reporter) is a gene that researchers attach to a regulatory sequence of another gene of interest in bacteria, cell culture, animals or plants.
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Restriction enzyme
A restriction enzyme or restriction endonuclease is an enzyme that cleaves DNA into fragments at or near specific recognition sites within the molecule known as restriction sites.
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Reverse transcriptase
A reverse transcriptase (RT) is an enzyme used to generate complementary DNA (cDNA) from an RNA template, a process termed reverse transcription.
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Saccharomyces cerevisiae
Saccharomyces cerevisiae is a species of yeast.
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Stanley Fields (biologist)
Stanley Fields is an American biologist best known for developing the yeast two hybrid method for identifying protein–protein interactions.
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STAT3
Signal transducer and activator of transcription 3 (STAT3) is a transcription factor which in humans is encoded by the STAT3 gene.
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Synthetic genetic array
Synthetic genetic array analysis (SGA) is a high-throughput technique for exploring synthetic lethal and synthetic sick genetic interactions (SSL).
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Takara Holdings
is a Japanese company based in Kyoto.
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TetR
Tet Repressor proteins (otherwise known as TetR) are proteins playing an important role in conferring antibiotic resistance to large categories of bacterial species.
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Transcription (biology)
Transcription is the first step of gene expression, in which a particular segment of DNA is copied into RNA (especially mRNA) by the enzyme RNA polymerase.
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Transcription factor
In molecular biology, a transcription factor (TF) (or sequence-specific DNA-binding factor) is a protein that controls the rate of transcription of genetic information from DNA to messenger RNA, by binding to a specific DNA sequence.
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Tyrosine kinase 2
Non-receptor tyrosine-protein kinase TYK2 is an enzyme that in humans is encoded by the TYK2 gene.
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Ubiquitin
Ubiquitin is a small (8.5 kDa) regulatory protein found in most tissues of eukaryotic organisms, i.e. it occurs ''ubiquitously''.
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Upstream activating sequence
An upstream activating sequence or upstream activation sequence (UAS) is a cis-acting regulatory sequence.
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Upstream and downstream (DNA)
In molecular biology and genetics, upstream and downstream both refer to relative positions of genetic code in DNA or RNA.
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Y187
Y187 is a strain of yeast (Saccharomyces cerevisiae) used in biological research for two-hybrid screening.
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Zinc finger
A zinc finger is a small protein structural motif that is characterized by the coordination of one or more zinc ions (Zn2+) in order to stabilize the fold.
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3-Amino-1,2,4-triazole
3-Amino-1,2,4-triazole (3-AT) is a heterocyclic organic compound that consists of a 1,2,4-triazole substituted with an amino group.
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References
[1] https://en.wikipedia.org/wiki/Two-hybrid_screening
