Monoclonal antibody and Western blot

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Difference between Monoclonal antibody and Western blot

Monoclonal antibody vs. Western blot

Monoclonal antibodies (mAb or moAb) are antibodies that are made by identical immune cells that are all clones of a unique parent cell. The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used in molecular biology, immunogenetics and other molecular biology disciplines to detect specific proteins in a sample of tissue homogenate or extract.

Antibody

An antibody (Ab), also known as an immunoglobulin (Ig), is a large, Y-shaped protein produced mainly by plasma cells that is used by the immune system to neutralize pathogens such as pathogenic bacteria and viruses.

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Biochemistry

Biochemistry, sometimes called biological chemistry, is the study of chemical processes within and relating to living organisms.

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Cloning

Cloning is the process of producing genetically identical individuals of an organism either naturally or artificially.

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Dot blot

A dot blot (or slot blot) is a technique in molecular biology used to detect proteins.

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ELISA

The enzyme-linked immunosorbent assay (ELISA) is a test that uses antibodies and color change to identify a substance.

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Hybridoma technology

Hybridoma technology is a method for producing large numbers of identical antibodies (also called monoclonal antibodies).

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Immunohistochemistry

Immunohistochemistry (IHC) involves the process of selectively imaging antigens (proteins) in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues.

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Isoelectric point

The isoelectric point (pI, pH(I), IEP), is the pH at which a particular molecule carries no net electrical charge or is electrically neutral in the statistical mean.

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Molecular biology

Molecular biology is a branch of biology which concerns the molecular basis of biological activity between biomolecules in the various systems of a cell, including the interactions between DNA, RNA, proteins and their biosynthesis, as well as the regulation of these interactions.

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Polyclonal antibodies

Polyclonal antibodies (pAbs) are antibodies that are secreted by different B cell lineages within the body (whereas monoclonal antibodies come from a single cell lineage).

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