Φ29 DNA polymerase

Φ29 DNA polymerase is an enzyme from the bacteriophage Φ29. ^[1]

8 relations: Bacillus phage phi29, Bacillus subtilis, Exonuclease, Helicase, Klenow fragment, Molecular biology, Multiple displacement amplification, Primer (molecular biology).

Bacillus phage phi29

Bacillus phage phi29 (Φ29 phage) belongs to a family of related Bacteriophages which includes, in addition to Φ29, phages PZA, Φ15, BS32, B103, M2Y (M2), Nf and GA-1.

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Bacillus subtilis

Bacillus subtilis, known also as the hay bacillus or grass bacillus, is a Gram-positive, catalase-positive bacterium, found in soil and the gastrointestinal tract of ruminants and humans.

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Exonuclease

Exonucleases are enzymes that work by cleaving nucleotides one at a time from the end (exo) of a polynucleotide chain.

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Helicase

Helicases are a class of enzymes vital to all living organisms.

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Klenow fragment

The Klenow fragment is a large protein fragment produced when DNA polymerase I from E. coli is enzymatically cleaved by the protease subtilisin.

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Molecular biology is a branch of biology which concerns the molecular basis of biological activity between biomolecules in the various systems of a cell, including the interactions between DNA, RNA, proteins and their biosynthesis, as well as the regulation of these interactions.

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Multiple displacement amplification

Multiple displacement amplification (MDA) is a non-PCR based DNA amplification technique.

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Primer (molecular biology)

A primer is a short strand of RNA or DNA (generally about 18-22 bases) that serves as a starting point for DNA synthesis.

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Redirects here:

Phi 29 DNA polymerase, Phi29 polymerase.

References

[1] https://en.wikipedia.org/wiki/Φ29_DNA_polymerase

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