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Multiplex polymerase chain reaction

Index Multiplex polymerase chain reaction

Multiplex polymerase chain reaction (Multiplex PCR) refers to the use of polymerase chain reaction to amplify several different DNA sequences simultaneously (as if performing many separate PCR reactions all together in one reaction). [1]

12 relations: Amplicon, DNA, DNA polymerase, DNA replication, Dystrophin, Gel electrophoresis, Microsatellite, Polymerase chain reaction, Primer (molecular biology), Single-nucleotide polymorphism, Steroid sulfatase, Thermal cycler.

Amplicon

In molecular biology, an amplicon is a piece of DNA or RNA that is the source and/or product of amplification or replication events.

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DNA

Deoxyribonucleic acid (DNA) is a thread-like chain of nucleotides carrying the genetic instructions used in the growth, development, functioning and reproduction of all known living organisms and many viruses.

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DNA polymerase

DNA polymerases are enzymes that synthesize DNA molecules from deoxyribonucleotides, the building blocks of DNA.

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DNA replication

In molecular biology, DNA replication is the biological process of producing two identical replicas of DNA from one original DNA molecule.

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Dystrophin

Dystrophin is a rod-shaped cytoplasmic protein, and a vital part of a protein complex that connects the cytoskeleton of a muscle fiber to the surrounding extracellular matrix through the cell membrane.

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Gel electrophoresis

Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge.

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Microsatellite

A microsatellite is a tract of repetitive DNA in which certain DNA motifs (ranging in length from 1–6 or more base pairs) are repeated, typically 5–50 times.

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Polymerase chain reaction

Polymerase chain reaction (PCR) is a technique used in molecular biology to amplify a single copy or a few copies of a segment of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence.

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Primer (molecular biology)

A primer is a short strand of RNA or DNA (generally about 18-22 bases) that serves as a starting point for DNA synthesis.

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Single-nucleotide polymorphism

A single-nucleotide polymorphism, often abbreviated to SNP (plural), is a variation in a single nucleotide that occurs at a specific position in the genome, where each variation is present to some appreciable degree within a population (e.g. > 1%).

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Steroid sulfatase

Steroid sulfatase (STS), or steryl-sulfatase, formerly known as arylsulfatase C, is a sulfatase enzyme involved in the metabolism of steroids.

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Thermal cycler

The thermal cycler (also known as a thermocycler, PCR machine or DNA amplifier) is a laboratory apparatus most commonly used to amplify segments of DNA via the polymerase chain reaction (PCR).

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Redirects here:

Multiplex PCR, Multiplex genomic PCR, Multiplex-PCR.

References

[1] https://en.wikipedia.org/wiki/Multiplex_polymerase_chain_reaction

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