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87 relations: Absolute molar mass, Agarose, Ammonium sulfate precipitation, Analytical light scattering, Anion-exchange chromatography, ASEC, Brookhaven Instruments, Calitoxin, Calprotectin, Characterization of nanoparticles, Chromatography detector, Clostridium difficile toxin B, Crystallin, Desalting and buffer exchange, Diethylaminoethyl cellulose, Differential refractometer, Dispersity, Displacement chromatography, Electrochromatography, Enoyl CoA isomerase, Fast protein liquid chromatography, Fluorescence in situ hybridization, Gel, Gel permeation chromatography, Granadaene, High-performance liquid chromatography, Hydrodynamic radius, Hydrogenase, IKBKAP, Inductively coupled plasma mass spectrometry, Instruments used in medical laboratories, Intrinsically disordered proteins, Ion chromatography, Jerker Porath, Juvenile hormone diol kinase, Keratinase, Kidney stone disease, Lignin characterization, Liquid chromatography–mass spectrometry, List of instruments used in toxicology, List of materials analysis methods, List of MeSH codes (E05), Low-angle laser light scattering, Macroprolactin, Mark–Houwink equation, Matrix-assisted laser desorption/ionization, Micellar liquid chromatography, Minigene, Molar mass distribution, Molecular engineering, ..., Molecular mass, Monoclonal antibody, Multiangle light scattering, N-alpha-acetyltransferase 10, NiFe hydrogenase, Oligonucleotide synthesis, Ophanin, Pandinus imperator (Pi3) toxin, PEGylation, Pharmacia, Phomoxanthone A, Photo-reactive amino acid analog, Phycocyanin, Polymer Char, Polymer characterization, Polymer fractionation, Polymer physics, Protein methods, Protein purification, Protein quaternary structure, Red Cell Affinity Column Technology, Residence time, Ribose-5-phosphate isomerase, S300, SDS-PAGE, SEC, Sephadex, Simulated moving bed, Star-shaped polymer, Superose, Taipoxin, Tamapin, Thermal shift assay, Thermoresponsive polymers in chromatography, Tosoh, Trehalase, 3-Arylpropiolonitriles. Expand index (37 more) »
Absolute molar mass
Absolute molar mass is a process used to determine the characteristics of molecules.
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Agarose
Agarose is a polysaccharide, generally extracted from certain red seaweed.
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Ammonium sulfate precipitation
Ammonium sulfate precipitation is one of the most commonly used methods for large and laboratory scale protein purification and fractionation that can be used to separate proteins by altering their solubility in the presence of a high salt concentration.
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Analytical light scattering
Analytical light scattering (ALS), also loosely referred to as SEC-MALS, is the implementation of static light scattering (SLS) and dynamic light scattering (DLS) techniques in an online or flow mode.
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Anion-exchange chromatography
Anion-exchange chromatography is a process that separates substances based on their charges using an ion-exchange resin containing positively charged groups, such as diethyl-aminoethyl groups (DEAE).
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ASEC
ASEC or asec may refer to.
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Brookhaven Instruments
Brookhaven Instruments Corporation is a Nova Instruments company.
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Calitoxin
Calitoxin, also known as CLX, is a sea anemone neurotoxin produced by the sea anemone Calliactis parasitica.
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Calprotectin
Calprotectin is a complex of the mammalian proteins S100A8 and S100A9.
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Characterization of nanoparticles
The characterization of nanoparticles is a branch of nanometrology that deals with the characterization of physical and chemical properties of nanoparticles.
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Chromatography detector
A chromatography detector is a device used in gas chromatography (GC) or liquid chromatography (LC) to detect components of the mixture being eluted off the chromatography column.
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Clostridium difficile toxin B
Clostridium difficile toxin B is a toxin produced by the bacteria Clostridium difficile.
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Crystallin
In anatomy, a crystallin is a water-soluble structural protein found in the lens and the cornea of the eye accounting for the transparency of the structure.
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Desalting and buffer exchange
Desalting and buffer exchange are methods to separate soluble macromolecules from smaller molecules (desalting) or replace the buffer system used for another one suitable for a downstream application (buffer exchange).
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Diethylaminoethyl cellulose
Diethylaminoethyl cellulose (DEAE-C) is a positively charged resin used in ion-exchange chromatography, a type of column chromatography, for the separation and purification of proteins and nucleic acids.
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Differential refractometer
A differential refractometer (DRI), or refractive index detector (RI or RID) is a detector that measures the refractive index of an analyte relative to the solvent.
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Dispersity
A monodisperse, or uniform, polymer is composed of molecules of the same mass.
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Displacement chromatography
Displacement chromatography is a chromatography technique in which a sample is placed onto the head of the column and is then displaced by a solute that is more strongly sorbed than the components of the original mixture.
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Electrochromatography
Electrochromatography is a chemical separation technique in analytical chemistry, biochemistry and molecular biology used to resolve and separate mostly large biomolecules such as proteins.
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Enoyl CoA isomerase
Enoyl-CoA-(∆) isomerase, also known as dodecenoyl-CoA-(∆) isomerase, 3,2-trans-enoyl-CoA isomerase, ∆3(cis),∆2(trans)-enoyl-CoA isomerase, or acetylene-allene isomerase, is an enzyme that catalyzes the conversion of cis-or trans-double bonds of fatty acids at gamma-carbon (position 3) to trans double bonds at beta-carbon (position 2).
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Fast protein liquid chromatography
Fast protein liquid chromatography (FPLC), is a form of liquid chromatography that is often used to analyze or purify mixtures of proteins. As in other forms of chromatography, separation is possible because the different components of a mixture have different affinities for two materials, a moving fluid (the "mobile phase") and a porous solid (the stationary phase). In FPLC the mobile phase is an aqueous solution, or "buffer". The buffer flow rate is controlled by a positive-displacement pump and is normally kept constant, while the composition of the buffer can be varied by drawing fluids in different proportions from two or more external reservoirs. The stationary phase is a resin composed of beads, usually of cross-linked agarose, packed into a cylindrical glass or plastic column. FPLC resins are available in a wide range of bead sizes and surface ligands depending on the application. In the most common FPLC strategy, ion exchange, a resin is chosen that the protein of interest will bind to the resin by a charge interaction while in buffer A (the running buffer) but become dissociated and return to solution in buffer B (the elution buffer). A mixture containing one or more proteins of interest is dissolved in 100% buffer A and pumped into the column. The proteins of interest bind to the resin while other components are carried out in the buffer. The total flow rate of the buffer is kept constant; however, the proportion of Buffer B (the "elution" buffer) is gradually increased from 0% to 100% according to a programmed change in concentration (the "gradient"). At some point during this process each of the bound proteins dissociates and appears in the effluent. The effluent passes through two detectors which measure salt concentration (by conductivity) and protein concentration (by absorption of ultraviolet light at a wavelength of 280nm). As each protein is eluted it appears in the effluent as a "peak" in protein concentration and can be collected for further use. FPLC was developed and marketed in Sweden by Pharmacia in 1982 and was originally called fast performance liquid chromatography to contrast it with HPLC or high-performance liquid chromatography. FPLC is generally applied only to proteins; however, because of the wide choice of resins and buffers it has broad applications. In contrast to HPLC the buffer pressure used is relatively low, typically less than 5 bar, but the flow rate is relatively high, typically 1-5 ml/min. FPLC can be readily scaled from analysis of milligrams of mixtures in columns with a total volume of 5ml or less to industrial production of kilograms of purified protein in columns with volumes of many liters. When used for analysis of mixtures the effluent is usually collected in fractions of 1-5 ml which can be further analyzed, e.g. by MALDI mass spectrometry. When used for protein purification there may be only two collection containers, one for the purified product and one for waste.
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Fluorescence in situ hybridization
Fluorescent in situ hybridization (FISH) is a molecular cytogenetic technique that uses fluorescent probes that bind to only those parts of the chromosome with a high degree of sequence complementarity.
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Gel
A gel is a solid jelly-like material that can have properties ranging from soft and weak to hard and tough.
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Gel permeation chromatography
Gel permeation chromatography (GPC) is a type of size exclusion chromatography (SEC), that separates analytes on the basis of size.
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Granadaene
Granadaene is the trivial name of a non-isoprenoid polyene that constitutes the red pigment characteristic of Streptococcus agalactiae (group B streptococcus).
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High-performance liquid chromatography
High-performance liquid chromatography (HPLC; formerly referred to as high-pressure liquid chromatography), is a technique in analytical chemistry used to separate, identify, and quantify each component in a mixture.
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Hydrodynamic radius
The hydrodynamic radius of a macromolecule or colloid particle is R_.
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Hydrogenase
A hydrogenase is an enzyme that catalyses the reversible oxidation of molecular hydrogen (H2), as shown below: Hydrogen uptake is coupled to the reduction of electron acceptors such as oxygen, nitrate, sulfate, carbon dioxide, and fumarate.
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IKBKAP
IKBKAP (inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex-associated protein) is a human gene encoding the IKAP protein, which is ubiquitously expressed at varying levels in all tissue types, including brain cells.
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Inductively coupled plasma mass spectrometry
Inductively coupled plasma mass spectrometry (ICP-MS) is a type of mass spectrometry which is capable of detecting metals and several non-metals at concentrations as low as one part in 1015 (part per quadrillion, ppq) on non-interfered low-background isotopes.
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Instruments used in medical laboratories
This is a list of instruments used in general in laboratories, including.
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Intrinsically disordered proteins
An intrinsically disordered protein (IDP) is a protein that lacks a fixed or ordered three-dimensional structure.
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Ion chromatography
Ion chromatography (or ion-exchange chromatography) is a chromatography process that separates ions and polar molecules based on their affinity to the ion exchanger.
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Jerker Porath
Jerker Porath, (23 October 1921 – 21 January 2016) was a Swedish biochemist who invented several separation methods for biomolecules.
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Juvenile hormone diol kinase
The conjugate (10S,11S) JH diol phosphate is the product of a two-step enzymatic process: conversion of JH to JH diol and then addition of a phosphate group to C10.
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Keratinase
Keratinases are proteolytic enzymes that digest keratin.
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Kidney stone disease
Kidney stone disease, also known as urolithiasis, is when a solid piece of material (kidney stone) occurs in the urinary tract.
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Lignin characterization
The term "Lignin characterization" (or "Lignin analysis") refers to a group of activities within lignin research aiming at describing the characteristics of a lignin by determination of its most important properties.
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Liquid chromatography–mass spectrometry
Liquid chromatography–mass spectrometry (LC-MS) is an analytical chemistry technique that combines the physical separation capabilities of liquid chromatography (or HPLC) with the mass analysis capabilities of mass spectrometry (MS).
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List of instruments used in toxicology
Instruments used specially in Toxicology are as follows.
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List of materials analysis methods
List of materials analysis methods.
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List of MeSH codes (E05)
The following is a list of the "E" codes for MeSH.
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Low-angle laser light scattering
Low-angle laser light scattering or LALLS is an application of light scattering that is particularly useful in conjunction with the technique of Size exclusion chromatography, one of the most powerful and widely used techniques to study the molecular mass distribution of a polymer.
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Macroprolactin
Macroprolactin is a physiologically inactive form of prolactin found in a small proportion of people.
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Mark–Houwink equation
The Mark–Houwink equation, also known as the Mark–Houwink–Sakurada equation or the Kuhn–Mark–Houwink–Sakurada equation or the Landau-Kuhn-Mark-Houwink-Sakurada equation gives a relation between intrinsic viscosity and molecular weight M: From this equation the molecular weight of a polymer can be determined from data on the intrinsic viscosity and vice versa.
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Matrix-assisted laser desorption/ionization
In mass spectrometry, matrix-assisted laser desorption/ionization (MALDI) is an ionization technique that uses a laser energy absorbing matrix to create ions from large molecules with minimal fragmentation.
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Micellar liquid chromatography
Micellar liquid chromatography (MLC) is a form of reversed phase liquid chromatography that uses an aqueous micellar solutions as the mobile phase.
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Minigene
A minigene is a minimal gene fragment that includes an exon and the control regions necessary for the gene to express itself in the same way as a wild type gene fragment.
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Molar mass distribution
In linear polymers the individual polymer chains rarely have exactly the same degree of polymerization and molar mass, and there is always a distribution around an average value.
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Molecular engineering
Molecular engineering is an emerging field of study concerned with the design and testing of molecular properties, behavior and interactions in order to assemble better materials, systems, and processes for specific functions.
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Molecular mass
Relative Molecular mass or molecular weight is the mass of a molecule.
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Monoclonal antibody
Monoclonal antibodies (mAb or moAb) are antibodies that are made by identical immune cells that are all clones of a unique parent cell.
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Multiangle light scattering
Multiangle light scattering (MALS) describes a technique for measuring the light scattered by a sample into a plurality of angles.
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N-alpha-acetyltransferase 10
N-alpha-acetyltransferase 10 (NAA10) also known as NatA catalytic subunit Naa10 and arrest-defective protein 1 homolog A (ARD1A) is an enzyme subunit that in humans is encoded NAA10 gene.
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NiFe hydrogenase
hydrogenase is a type of hydrogenase, which is an oxidative enzyme that reversibly convert molecular hydrogen in prokaryotes including Bacteria and Archaea.
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Oligonucleotide synthesis
Oligonucleotide synthesis is the chemical synthesis of relatively short fragments of nucleic acids with defined chemical structure (sequence).
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Ophanin
Ophanin is a toxin found in the venom of the King Cobra (Ophiophagus hannah), which lives throughout South East Asia.
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Pandinus imperator (Pi3) toxin
Pi3 toxin is a purified peptide derivative of the ''Pandinus imperator'' scorpion venom.
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PEGylation
PEGylation (often styled pegylation) is the process of both covalent and non-covalent attachment or amalgamation of polyethylene glycol (PEG, in pharmacy called macrogol) polymer chains to molecules and macrostructures, such as a drug, therapeutic protein or vesicle, which is then described as PEGylated (pegylated).
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Pharmacia
Pharmacia was a pharmaceutical and biotechnological company in Sweden that merged with the American pharmaceutical company Upjohn in 1995.
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Phomoxanthone A
The mycotoxin phomoxanthone A, or PXA for short, is a toxic natural product that affects the mitochondria.
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Photo-reactive amino acid analog
Photo-reactive amino acid analogs are artificial analogs of natural amino acids that can be used for crosslinking of protein complexes.
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Phycocyanin
Phycocyanin is a pigment-protein complex from the light-harvesting phycobiliprotein family, along with allophycocyanin and phycoerythrin.
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Polymer Char
Polymer Char is a company which designs and manufactures instrumentation for polymer analysis.
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Polymer characterization
Polymer characterization is the analytical branch of polymer science.
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Polymer fractionation
Polymers are chainlike molecules that are made of the same repetition unit.
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Polymer physics
Polymer physics is the field of physics that studies polymers, their fluctuations, mechanical properties, as well as the kinetics of reactions involving degradation and polymerisation of polymers and monomers respectively.
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Protein methods
Protein methods are the techniques used to study proteins.
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Protein purification
Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms.
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Protein quaternary structure
Protein quaternary structure is the number and arrangement of multiple folded protein subunits in a multi-subunit complex.
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Red Cell Affinity Column Technology
It is a method for detecting any clinically important antibodies in patient serum.
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Residence time
For material flowing through a volume, the residence time is a measure of how much time the matter spends in it.
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Ribose-5-phosphate isomerase
Ribose-5-phosphate isomerase (Rpi) encoded by the RPIA gene is an enzyme that catalyzes the conversion between ribose-5-phosphate (R5P) and ribulose-5-phosphate (Ru5P).
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S300
S300 may refer to.
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SDS-PAGE
SDS-PAGE (sodium dodecyl sulfate–polyacrylamide gel electrophoresis) is a variant of polyacrylamide gel electrophoresis, an analytical method in biochemistry for the separation of charged molecules in mixtures by their molecular masses in an electric field.
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SEC
SEC, Sec, or Seč may refer to.
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Sephadex
Sephadex is a trademark for cross-linked dextran gel used for gel filtration.
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Simulated moving bed
In manufacturing, the simulated moving bed (SMB) process is a highly engineered process for implementing chromatographic separation.
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Star-shaped polymer
Star-shaped polymers are the simplest class of branched polymers with a general structure consisting of several (more than three) linear chains connected to a central core.
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Superose
Superose is a trade name for a collection of FPLC columns which are used in the automated separation of biological molecules.
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Taipoxin
Taipoxin is a potent myo- and neurotoxin, which was isolated from the venom of the coastal taipan Oxyuranus scutellatus or also known as the common taipan.
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Tamapin
Tamapin is a toxin from the Indian Red Scorpion (Mesobuthus tamalus), which is a selective and potent blocker of SK2 channels.
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Thermal shift assay
A thermal shift assay quantifies the change in thermal denaturation temperature of a protein under varying conditions.
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Thermoresponsive polymers in chromatography
Thermoresponsive polymers can be used as stationary phase in liquid chromatography.
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Tosoh
is a global chemical and specialty materials company.
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Trehalase
Trehalase is a glycoside hydrolase enzyme located in on the brush border of the small intestine that catalyzes the conversion of trehalose to glucose.
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3-Arylpropiolonitriles
3-Arylpropiolonitriles (APN) belong to a class of electron-deficient alkyne derivatives substituted by two electron-withdrawing groups – a nitrile and an aryl moieties.
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Absolute Size Exclusion Chromatography, Absolute size exclusion chromatography, Absolute size-exclusion chromatography, Chromatography, gel, Gel Chromatography, Gel filtration, Gel filtration chromatography, Gel-filtration chromatography, HPSEC, Molecular-sieve chomatography, Size Exclusion Chromatography, Size exclusion, Size exclusion chromatography, Size-Exclusion Chromatography.
References
[1] https://en.wikipedia.org/wiki/Size-exclusion_chromatography
