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37 relations: Applied Biosystems, Autoradiograph, Buffer solution, Capillary electrophoresis, Chromatography, Cloning, Cloning vector, Denaturation (biochemistry), Dideoxynucleotide, DNA polymerase, DNA replication, DNA sequencer, DNA sequencing, Fluorescence, Frederick Sanger, Gel electrophoresis, Genome, Heteroduplex, Hour, Hydroxy group, In vitro, Isotopic labeling, Lab-on-a-chip, Leroy Hood, Microsatellite, Nucleotide, Phosphodiester bond, Phred base calling, Polyacrylamide gel electrophoresis, Polymerase chain reaction, Primer (molecular biology), Pyrosequencing, Radiography, Single-nucleotide polymorphism, Single-strand conformation polymorphism, Thermal cycler, Wavelength.
Applied Biosystems
Applied Biosystems is one of the various brands under the Life Technologies brand of Thermo Fisher Scientific corporation.
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Autoradiograph
An autoradiograph is an image on an x-ray film or nuclear emulsion produced by the pattern of decay emissions (e.g., beta particles or gamma rays) from a distribution of a radioactive substance.
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Buffer solution
A buffer solution (more precisely, pH buffer or hydrogen ion buffer) is an aqueous solution consisting of a mixture of a weak acid and its conjugate base, or vice versa.
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Capillary electrophoresis
Capillary electrophoresis (CE) is a family of electrokinetic separation methods performed in submillimeter diameter capillaries and in micro- and nanofluidic channels.
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Chromatography
Chromatography is a laboratory technique for the separation of a mixture.
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Cloning
Cloning is the process of producing genetically identical individuals of an organism either naturally or artificially.
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Cloning vector
A cloning vector is a small piece of DNA, taken from a virus, a plasmid, or the cell of a higher organism, that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes.
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Denaturation (biochemistry)
Denaturation is a process in which proteins or nucleic acids lose the quaternary structure, tertiary structure, and secondary structure which is present in their native state, by application of some external stress or compound such as a strong acid or base, a concentrated inorganic salt, an organic solvent (e.g., alcohol or chloroform), radiation or heat.
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Dideoxynucleotide
Dideoxynucleotides are chain-elongating inhibitors of DNA polymerase, used in the Sanger method for DNA sequencing.
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DNA polymerase
DNA polymerases are enzymes that synthesize DNA molecules from deoxyribonucleotides, the building blocks of DNA.
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DNA replication
In molecular biology, DNA replication is the biological process of producing two identical replicas of DNA from one original DNA molecule.
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DNA sequencer
A DNA sequencer is a scientific instrument used to automate the DNA sequencing process.
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DNA sequencing
DNA sequencing is the process of determining the precise order of nucleotides within a DNA molecule.
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Fluorescence
Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation.
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Frederick Sanger
Frederick Sanger (13 August 1918 – 19 November 2013) was a British biochemist who twice won the Nobel Prize in Chemistry, one of only two people to have done so in the same category (the other is John Bardeen in physics), the fourth person overall with two Nobel Prizes, and the third person overall with two Nobel Prizes in the sciences.
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Gel electrophoresis
Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge.
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Genome
In the fields of molecular biology and genetics, a genome is the genetic material of an organism.
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Heteroduplex
A heteroduplex is a double-stranded (duplex) molecule of nucleic acid originated through the genetic recombination of single complementary strands derived from different sources, such as from different homologous chromosomes or even from different organisms.
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Hour
An hour (symbol: h; also abbreviated hr.) is a unit of time conventionally reckoned as of a day and scientifically reckoned as 3,599–3,601 seconds, depending on conditions.
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Hydroxy group
A hydroxy or hydroxyl group is the entity with the formula OH.
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In vitro
In vitro (meaning: in the glass) studies are performed with microorganisms, cells, or biological molecules outside their normal biological context.
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Isotopic labeling
Isotopic labeling (or isotopic labelling) is a technique used to track the passage of an isotope (an atom with a detectable variation) through a reaction, metabolic pathway, or cell.
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Lab-on-a-chip
A lab-on-a-chip (LOC) is a device that integrates one or several laboratory functions on a single integrated circuit (commonly called a "chip") of only millimeters to a few square centimeters to achieve automation and high-throughput screening.
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Leroy Hood
Leroy "Lee" Edward Hood (born October 10, 1938) is an American biologist who has served on the faculties at the California Institute of Technology (Caltech) and the University of Washington.
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Microsatellite
A microsatellite is a tract of repetitive DNA in which certain DNA motifs (ranging in length from 1–6 or more base pairs) are repeated, typically 5–50 times.
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Nucleotide
Nucleotides are organic molecules that serve as the monomer units for forming the nucleic acid polymers deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), both of which are essential biomolecules within all life-forms on Earth.
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Phosphodiester bond
A phosphodiester bond occurs when exactly two of the hydroxyl groups in phosphoric acid react with hydroxyl groups on other molecules to form two ester bonds.
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Phred base calling
Phred base-calling is a computer program for identifying a base (nucleobase) sequence from a fluorescence "trace" data generated by an automated DNA sequencer that uses electrophoresis and 4-fluorescent dye method.
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Polyacrylamide gel electrophoresis
Polyacrylamide gel electrophoresis (PAGE) is a technique widely used in biochemistry, forensic chemistry, genetics, molecular biology and biotechnology to separate biological macromolecules, usually proteins or nucleic acids, according to their electrophoretic mobility.
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Polymerase chain reaction
Polymerase chain reaction (PCR) is a technique used in molecular biology to amplify a single copy or a few copies of a segment of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence.
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Primer (molecular biology)
A primer is a short strand of RNA or DNA (generally about 18-22 bases) that serves as a starting point for DNA synthesis.
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Pyrosequencing
Pyrosequencing is a method of DNA sequencing (determining the order of nucleotides in DNA) based on the "sequencing by synthesis" principle, in which the sequencing is performed by detecting the nucleotide incorporated by a DNA polymerase.
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Radiography
Radiography is an imaging technique using X-rays to view the internal form of an object.
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Single-nucleotide polymorphism
A single-nucleotide polymorphism, often abbreviated to SNP (plural), is a variation in a single nucleotide that occurs at a specific position in the genome, where each variation is present to some appreciable degree within a population (e.g. > 1%).
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Single-strand conformation polymorphism
Single-strand conformation polymorphism (SSCP), or single-strand chain polymorphism, is defined as a conformational difference of single-stranded nucleotide sequences of identical length as induced by differences in the sequences under certain experimental conditions.
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Thermal cycler
The thermal cycler (also known as a thermocycler, PCR machine or DNA amplifier) is a laboratory apparatus most commonly used to amplify segments of DNA via the polymerase chain reaction (PCR).
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Wavelength
In physics, the wavelength is the spatial period of a periodic wave—the distance over which the wave's shape repeats.
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Chain termination method, Chain-termination method, Dideoxy Method, Dideoxy method, Dideoxy termination, Microfluidic Sanger Sequencing, Microfluidic Sanger sequencing, Sanger Method, Sanger method, Sanger sequence, Sanger-Sequencing.
References
[1] https://en.wikipedia.org/wiki/Sanger_sequencing
