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Edman degradation

Index Edman degradation

Edman degradation, developed by Pehr Edman, is a method of sequencing amino acids in a peptide. [1]

17 relations: Acetylation, Acid, Amino acid, Bergmann degradation, Chromatography, Dansyl chloride, Electrophoresis, Isoaspartate, Pehr Victor Edman, Peptide, Peptide bond, Phenyl isothiocyanate, Pico-, Protein sequencing, Pyroglutamic acid, Residue (chemistry), Sequence.

Acetylation

Acetylation (or in IUPAC nomenclature ethanoylation) describes a reaction that introduces an acetyl functional group into a chemical compound.

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Acid

An acid is a molecule or ion capable of donating a hydron (proton or hydrogen ion H+), or, alternatively, capable of forming a covalent bond with an electron pair (a Lewis acid).

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Amino acid

Amino acids are organic compounds containing amine (-NH2) and carboxyl (-COOH) functional groups, along with a side chain (R group) specific to each amino acid.

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Bergmann degradation

The Bergmann degradation is a series of chemical reactions designed to remove a single amino acid from the carboxylic acid (C-terminal) end of a peptide.

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Chromatography

Chromatography is a laboratory technique for the separation of a mixture.

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Dansyl chloride

Dansyl chloride or 5-(DimethylAmino)Naphthalene-1-SulfonYL chloride is a reagent that reacts with primary amino groups in both aliphatic and aromatic amines to produce stable blue- or blue-green–fluorescent sulfonamide adducts.

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Electrophoresis

Electrophoresis (from the Greek "Ηλεκτροφόρηση" meaning "to bear electrons") is the motion of dispersed particles relative to a fluid under the influence of a spatially uniform electric field.

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Isoaspartate

Isoaspartic acid (isoaspartate) is an amino acid that is an isomer of aspartic acid.

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Pehr Victor Edman

Pehr Victor Edman (April 14, 1916 — March 19, 1977) was a Swedish biochemist.

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Peptide

Peptides (from Gr.: πεπτός, peptós "digested"; derived from πέσσειν, péssein "to digest") are short chains of amino acid monomers linked by peptide (amide) bonds.

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Peptide bond

A peptide bond is a covalent chemical bond linking two consecutive amino acid monomers along a peptide or protein chain.

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Phenyl isothiocyanate

Phenyl isothiocyanate (PITC) is a reagent used in reversed phase HPLC.

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Pico-

Pico- (symbol p) is a unit prefix in the metric system denoting one trillionth, a factor of 10−12.

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Protein sequencing

Protein sequencing is the practical process of determining the amino acid sequence of all or part of a protein or peptide.

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Pyroglutamic acid

Pyroglutamic acid (also known as PCA, 5-oxoproline, pidolic acid, or pyroglutamate for its basic form) is a ubiquitous but little studied natural amino acid derivative in which the free amino group of glutamic acid or glutamine cyclizes to form a lactam.

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Residue (chemistry)

In chemistry residue is whatever remains or acts as a contaminant after a given class of events.

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Sequence

In mathematics, a sequence is an enumerated collection of objects in which repetitions are allowed.

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Edman Degredation, Edman sequencing.

References

[1] https://en.wikipedia.org/wiki/Edman_degradation

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