22 relations: Affinity chromatography, Cell (biology), Chloramphenicol, Chloroform, Chromatin immunoprecipitation, Complementary DNA, Cycloheximide, Messenger RNA, Microarray, Nicholas Ingolia, Nuclease, Nucleoside triphosphate, Phenol, Polysome, Polysome profiling, Reverse transcriptase, Ribosome, RNA-Seq, Sequencing, Transcriptome, Translation (biology), Translatome.
Affinity chromatography
Affinity chromatography is a method of separating biochemical mixtures based on a highly specific interaction between antigen and antibody, enzyme and substrate, receptor and ligand, or protein and nucleic acid.
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Cell (biology)
The cell (from Latin cella, meaning "small room") is the basic structural, functional, and biological unit of all known living organisms.
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Chloramphenicol
Chloramphenicol is an antibiotic useful for the treatment of a number of bacterial infections.
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Chloroform
Chloroform, or trichloromethane, is an organic compound with formula CHCl3.
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Chromatin immunoprecipitation
Chromatin immunoprecipitation (ChIP) is a type of immunoprecipitation experimental technique used to investigate the interaction between proteins and DNA in the cell.
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Complementary DNA
In genetics, complementary DNA (cDNA) is DNA synthesized from a single stranded RNA (e.g., messenger RNA (mRNA) or microRNA) template in a reaction catalyzed by the enzyme reverse transcriptase.
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Cycloheximide
Cycloheximide is a eukaryote protein synthesis inhibitor, produced by the bacterium Streptomyces griseus.
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Messenger RNA
Messenger RNA (mRNA) is a large family of RNA molecules that convey genetic information from DNA to the ribosome, where they specify the amino acid sequence of the protein products of gene expression.
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Microarray
A microarray is a multiplex lab-on-a-chip.
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Nicholas Ingolia
Nicholas Ingolia is an assistant professor at University of California, Berkeley in molecular biology.
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Nuclease
A nuclease (also archaically known as nucleodepolymerase or polynucleotidase) is an enzyme capable of cleaving the phosphodiester bonds between monomers of nucleic acids.
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Nucleoside triphosphate
A nucleoside triphosphate is a molecule containing a nitrogenous base bound to a 5-carbon sugar (either ribose or deoxyribose), with three phosphate groups bound to the sugar.
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Phenol
Phenol, also known as phenolic acid, is an aromatic organic compound with the molecular formula C6H5OH.
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Polysome
A polyribosome (or polysome) is a complex of an mRNA molecule and two or more ribosomes that act to translate mRNA instructions into polypeptides.
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Polysome profiling
Polysome profiling is a technique in molecular biology that is used to study the association of mRNAs with ribosomes.
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Reverse transcriptase
A reverse transcriptase (RT) is an enzyme used to generate complementary DNA (cDNA) from an RNA template, a process termed reverse transcription.
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Ribosome
The ribosome is a complex molecular machine, found within all living cells, that serves as the site of biological protein synthesis (translation).
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RNA-Seq
RNA-Seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), uses next-generation sequencing (NGS) to reveal the presence and quantity of RNA in a biological sample at a given moment.
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Sequencing
In genetics and biochemistry, sequencing means to determine the primary structure (sometimes falsely called primary sequence) of an unbranched biopolymer.
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Transcriptome
The transcriptome is the set of all RNA molecules in one cell or a population of cells.
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Translation (biology)
In molecular biology and genetics, translation is the process in which ribosomes in the cytoplasm or ER synthesize proteins after the process of transcription of DNA to RNA in the cell's nucleus.
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Translatome
The translatome is composed of all mRNA fragments that are translated in a moment or condition in a single cell.
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References
[1] https://en.wikipedia.org/wiki/Ribosome_profiling