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Rolling circle replication

Index Rolling circle replication

Rolling circle replication describes a process of unidirectional nucleic acid replication that can rapidly synthesize multiple copies of circular molecules of DNA or RNA, such as plasmids, the genomes of bacteriophages, and the circular RNA genome of viroids. [1]

43 relations: Aptamer, Avsunviroidae, Bacteriophage, Biosensor, Circular RNA, Concatemer, Directionality (molecular biology), DNA, DNA ligase, DNA polymerase, DNA polymerase I, DNA polymerase III holoenzyme, DNA replication, DNA virus, ELISA, Enterobacteria phage T4, Fluorophore, Gel, Gel electrophoresis, Geminiviridae, Genome, Human herpesvirus 6, Human papillomavirus infection, Immunoassay, Immunohistochemistry, Infection, Φ29 DNA polymerase, Microarray analysis techniques, Molecular beacon, Molecular biology, Nanotechnology, Nanowire, National Center for Biotechnology Information, Nucleic acid test, Okazaki fragments, Plasmid, Polymerase chain reaction, Pospiviroidae, Primer (molecular biology), Ribozyme, RNA, RNA polymerase, Viroid.

Aptamer

Aptamers (from the Latin aptus – fit, and Greek meros – part) are oligonucleotide or peptide molecules that bind to a specific target molecule.

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Avsunviroidae

The Avsunviroidae are a family of viroids.

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Bacteriophage

A bacteriophage, also known informally as a phage, is a virus that infects and replicates within Bacteria and Archaea.

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Biosensor

A biosensor is an analytical device, used for the detection of an analyte, that combines a biological component with a physicochemical detector.

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Circular RNA

Circular RNA (or circRNA) is a type of RNA which, unlike the better known linear RNA, forms a covalently closed continuous loop, i.e., in circular RNA the 3' and 5' ends normally present in an RNA molecule have been joined together.

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Concatemer

A concatemer is a long continuous DNA molecule that contains multiple copies of the same DNA sequence linked in series.

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Directionality (molecular biology)

Directionality, in molecular biology and biochemistry, is the end-to-end chemical orientation of a single strand of nucleic acid.

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DNA

Deoxyribonucleic acid (DNA) is a thread-like chain of nucleotides carrying the genetic instructions used in the growth, development, functioning and reproduction of all known living organisms and many viruses.

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DNA ligase

DNA ligase is a specific type of enzyme, a ligase, that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond.

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DNA polymerase

DNA polymerases are enzymes that synthesize DNA molecules from deoxyribonucleotides, the building blocks of DNA.

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DNA polymerase I

DNA polymerase I (or Pol I) is an enzyme that participates in the process of prokaryotic DNA replication.

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DNA polymerase III holoenzyme

DNA polymerase III holoenzyme is the primary enzyme complex involved in prokaryotic DNA replication.

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DNA replication

In molecular biology, DNA replication is the biological process of producing two identical replicas of DNA from one original DNA molecule.

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DNA virus

A DNA virus is a virus that has DNA as its genetic material and replicates using a DNA-dependent DNA polymerase.

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ELISA

The enzyme-linked immunosorbent assay (ELISA) is a test that uses antibodies and color change to identify a substance.

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Enterobacteria phage T4

Enterobacteria phage T4 is a bacteriophage that infects Escherichia coli bacteria.

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Fluorophore

A fluorophore (or fluorochrome, similarly to a chromophore) is a fluorescent chemical compound that can re-emit light upon light excitation.

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Gel

A gel is a solid jelly-like material that can have properties ranging from soft and weak to hard and tough.

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Gel electrophoresis

Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge.

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Geminiviridae

Geminiviridae is a family of plant viruses.

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Genome

In the fields of molecular biology and genetics, a genome is the genetic material of an organism.

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Human herpesvirus 6

Human herpesvirus 6 (HHV-6) is the common collective name for human herpesvirus 6A (HHV-6A) and human herpesvirus 6B (HHV-6B).

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Human papillomavirus infection

Human papillomavirus infection is an infection by human papillomavirus (HPV).

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Immunoassay

An immunoassay is a biochemical test that measures the presence or concentration of a macromolecule or a small molecule in a solution through the use of an antibody (usually) or an antigen (sometimes).

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Immunohistochemistry

Immunohistochemistry (IHC) involves the process of selectively imaging antigens (proteins) in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues.

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Infection

Infection is the invasion of an organism's body tissues by disease-causing agents, their multiplication, and the reaction of host tissues to the infectious agents and the toxins they produce.

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Φ29 DNA polymerase

Φ29 DNA polymerase is an enzyme from the bacteriophage Φ29.

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Microarray analysis techniques

Microarray analysis techniques are used in interpreting the data generated from experiments on DNA, RNA, and protein microarrays, which allow researchers to investigate the expression state of a large number of genes - in many cases, an organism's entire genome - in a single experiment.

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Molecular beacon

Molecular beacons are oligonucleotide hybridization probes that can report the presence of specific nucleic acids in homogenous solutions.

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Molecular biology

Molecular biology is a branch of biology which concerns the molecular basis of biological activity between biomolecules in the various systems of a cell, including the interactions between DNA, RNA, proteins and their biosynthesis, as well as the regulation of these interactions.

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Nanotechnology

Nanotechnology ("nanotech") is manipulation of matter on an atomic, molecular, and supramolecular scale.

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Nanowire

A nanowire is a nanostructure, with the diameter of the order of a nanometer (10−9 meters).

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National Center for Biotechnology Information

The National Center for Biotechnology Information (NCBI) is part of the United States National Library of Medicine (NLM), a branch of the National Institutes of Health (NIH).

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Nucleic acid test

A nucleic acid test (NAT) or nucleic acid amplification test (NAAT) is a technique utilized to detect a particular nucleic acid, virus, or bacteria which acts as a pathogen in blood, tissue, urine, etc.

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Okazaki fragments

Okazaki fragments are short, newly synthesized DNA fragments that are formed on the lagging template strand during DNA replication.

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Plasmid

A plasmid is a small DNA molecule within a cell that is physically separated from a chromosomal DNA and can replicate independently.

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Polymerase chain reaction

Polymerase chain reaction (PCR) is a technique used in molecular biology to amplify a single copy or a few copies of a segment of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence.

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Pospiviroidae

The Pospiviroidae are a family of viroids, including the first viroid to be discovered, PSTVd.

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Primer (molecular biology)

A primer is a short strand of RNA or DNA (generally about 18-22 bases) that serves as a starting point for DNA synthesis.

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Ribozyme

Ribozymes (ribonucleic acid enzymes) are RNA molecules that are capable of catalyzing specific biochemical reactions, similar to the action of protein enzymes.

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RNA

Ribonucleic acid (RNA) is a polymeric molecule essential in various biological roles in coding, decoding, regulation, and expression of genes.

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RNA polymerase

RNA polymerase (ribonucleic acid polymerase), both abbreviated RNAP or RNApol, official name DNA-directed RNA polymerase, is a member of a family of enzymes that are essential to life: they are found in all organisms (-species) and many viruses.

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Viroid

Viroids are the smallest infectious pathogens known.

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Redirects here:

Rolling Circle Replication, Rolling circle, Rolling circle DNA replication, Rolling circle amplification.

References

[1] https://en.wikipedia.org/wiki/Rolling_circle_replication

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